Tags

Type your tag names separated by a space and hit enter

Human telomerase reverse transcriptase (hTERT) gene expression in FNA samples from thyroid neoplasms.
Surgery. 1999 Dec; 126(6):1195-8; discussion 1198-9.S

Abstract

BACKGROUND

Although fine-needle aspiration (FNA) is the most sensitive method for the detection of thyroid carcinoma, it cannot provide a definitive diagnosis of malignancy in 60% of the patients operated on for suspicious lesions. Recently, human telomerase reverse transcriptase (hTERT) has been found to be a diagnostic marker of malignancy. We therefore sought to determine whether hTERT gene expression could serve as an adjunct to FNA in the differential diagnosis of thyroid nodules.

METHODS

Twenty-four FNA samples from thyroid nodules that were suspected of malignancy were collected. RNA was extracted, and hTERT gene expression was examined by RT-PCR. Cytologic and histologic examinations were also performed.

RESULTS

Two of three follicular, three of three Hürthle cell, and eight of eight papillary thyroid carcinomas had corresponding FNA samples that were positive for hTERT. One of two Hürthle cell adenomas was hTERT positive. FNA samples from three follicular adenomas and five hyperplastic nodules were negative for hTERT. Positive and negative predictive values were 93% and 90%, respectively.

CONCLUSIONS

The detection of hTERT gene expression in thyroid FNA samples holds promise as a diagnostic marker in the distinction of benign from malignant thyroid lesions. Its application could alter the surgical management of these patients.

Authors+Show Affiliations

Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD 21287-8611, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10598207

Citation

Zeiger, M A., et al. "Human Telomerase Reverse Transcriptase (hTERT) Gene Expression in FNA Samples From Thyroid Neoplasms." Surgery, vol. 126, no. 6, 1999, pp. 1195-8; discussion 1198-9.
Zeiger MA, Smallridge RC, Clark DP, et al. Human telomerase reverse transcriptase (hTERT) gene expression in FNA samples from thyroid neoplasms. Surgery. 1999;126(6):1195-8; discussion 1198-9.
Zeiger, M. A., Smallridge, R. C., Clark, D. P., Liang, C. K., Carty, S. E., Watson, C. G., Udelsman, R., & Saji, M. (1999). Human telomerase reverse transcriptase (hTERT) gene expression in FNA samples from thyroid neoplasms. Surgery, 126(6), 1195-8; discussion 1198-9.
Zeiger MA, et al. Human Telomerase Reverse Transcriptase (hTERT) Gene Expression in FNA Samples From Thyroid Neoplasms. Surgery. 1999;126(6):1195-8; discussion 1198-9. PubMed PMID: 10598207.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human telomerase reverse transcriptase (hTERT) gene expression in FNA samples from thyroid neoplasms. AU - Zeiger,M A, AU - Smallridge,R C, AU - Clark,D P, AU - Liang,C K, AU - Carty,S E, AU - Watson,C G, AU - Udelsman,R, AU - Saji,M, PY - 1999/12/22/pubmed PY - 1999/12/22/medline PY - 1999/12/22/entrez SP - 1195-8; discussion 1198-9 JF - Surgery JO - Surgery VL - 126 IS - 6 N2 - BACKGROUND: Although fine-needle aspiration (FNA) is the most sensitive method for the detection of thyroid carcinoma, it cannot provide a definitive diagnosis of malignancy in 60% of the patients operated on for suspicious lesions. Recently, human telomerase reverse transcriptase (hTERT) has been found to be a diagnostic marker of malignancy. We therefore sought to determine whether hTERT gene expression could serve as an adjunct to FNA in the differential diagnosis of thyroid nodules. METHODS: Twenty-four FNA samples from thyroid nodules that were suspected of malignancy were collected. RNA was extracted, and hTERT gene expression was examined by RT-PCR. Cytologic and histologic examinations were also performed. RESULTS: Two of three follicular, three of three Hürthle cell, and eight of eight papillary thyroid carcinomas had corresponding FNA samples that were positive for hTERT. One of two Hürthle cell adenomas was hTERT positive. FNA samples from three follicular adenomas and five hyperplastic nodules were negative for hTERT. Positive and negative predictive values were 93% and 90%, respectively. CONCLUSIONS: The detection of hTERT gene expression in thyroid FNA samples holds promise as a diagnostic marker in the distinction of benign from malignant thyroid lesions. Its application could alter the surgical management of these patients. SN - 0039-6060 UR - https://www.unboundmedicine.com/medline/citation/10598207/Human_telomerase_reverse_transcriptase__hTERT__gene_expression_in_FNA_samples_from_thyroid_neoplasms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-6060(99)70081-4 DB - PRIME DP - Unbound Medicine ER -