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Induction of neuronal and inducible nitric oxide synthase in the motoneurons of spinal cord following transient abdominal aorta occlusion in rats.
J Surg Res. 1999 Dec; 87(2):185-93.JS

Abstract

BACKGROUND

Motoneurons in the spinal cord are especially vulnerable to ischemic injury and selectively destroyed after transient ischemia. Nitric oxide (NO) has been implicated in both neurodegneration and neuroprotection to ischemic insult. To evaluate the role of NO in pathophysiology to spinal cord ischemia, the expression of neuronal and inducible nitric oxide synthase (n-NOS and i-NOS) and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) in the motoneurons of the lumbosacral spinal cord was examined in a rat model with transient abdominal aorta (TAA) occlusion.

MATERIALS AND METHODS

Male Sprague-Dawley rats were divided into sham-operated (n = 12) and TAA occlusion (n = 24) groups. TAA occlusion was induced by placement of a microvascular clamp around the abdominal aorta for 20 min. Three sham-operated and six TAA occlusion animals were sacrificed at each time interval at 4, 24, and 48 h and 7 days after operation. Tissue sections obtained from the lumbosacral spinal cord were processed for n-NOS, i-NOS, NADPH-d, and hematoxylin-eosin (HE) staining. Histological changes of motoneurons in ventral horn were assessed by HE staining.

RESULTS

In sham-operated control animals, n-NOS-, i-NOS-, and NADPH-d-positive neurons were barely detectable in the ventral horn of the spinal cord. At 4 h after TTA occlusion, n-NOS and NADPH-d expression became evident in the motoneurons and was markedly enhanced at 24 and 48 h. i-NOS expression was also induced in the ventral horn motoneurons of the lumbosacral spinal cord at the same time points. Enzymatic expression in the motoneurons was diminished 7 days after operation. Hyperchromatic neurons indicative of cell death were observed in HE-stained specimens 7 days following TAA occlusion.

CONCLUSIONS

The rapid induction of n-NOS, i-NOS, and NADPH-d in the motoneurons of ventral horn suggests that NO may be involved in the selective and delayed neuronal death in the spinal cord to the ischemic insult.

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Publisher Full Text

Authors+Show Affiliations

Zhou Y
Department of Experimental Surgery, Singapore General Hospital, 169608, Singapore. zhouy_twh@yahoo.com
Zhao YN
No affiliation info available
Yang EB
No affiliation info available
Ling EA
No affiliation info available
Wang Y
No affiliation info available
Hassouna MM
No affiliation info available
Mack P
No affiliation info available

MeSH

AnimalsAorta, AbdominalArterial Occlusive DiseasesEnzyme InductionImmunohistochemistryIschemiaMaleMotor NeuronsNADPH DehydrogenaseNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type INitric Oxide Synthase Type IIRatsRats, Sprague-DawleySpinal Cord

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10600348

Citation

Zhou, Y, et al. "Induction of Neuronal and Inducible Nitric Oxide Synthase in the Motoneurons of Spinal Cord Following Transient Abdominal Aorta Occlusion in Rats." The Journal of Surgical Research, vol. 87, no. 2, 1999, pp. 185-93.
Zhou Y, Zhao YN, Yang EB, et al. Induction of neuronal and inducible nitric oxide synthase in the motoneurons of spinal cord following transient abdominal aorta occlusion in rats. J Surg Res. 1999;87(2):185-93.
Zhou, Y., Zhao, Y. N., Yang, E. B., Ling, E. A., Wang, Y., Hassouna, M. M., & Mack, P. (1999). Induction of neuronal and inducible nitric oxide synthase in the motoneurons of spinal cord following transient abdominal aorta occlusion in rats. The Journal of Surgical Research, 87(2), 185-93.
Zhou Y, et al. Induction of Neuronal and Inducible Nitric Oxide Synthase in the Motoneurons of Spinal Cord Following Transient Abdominal Aorta Occlusion in Rats. J Surg Res. 1999;87(2):185-93. PubMed PMID: 10600348.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of neuronal and inducible nitric oxide synthase in the motoneurons of spinal cord following transient abdominal aorta occlusion in rats. AU - Zhou,Y, AU - Zhao,Y N, AU - Yang,E B, AU - Ling,E A, AU - Wang,Y, AU - Hassouna,M M, AU - Mack,P, PY - 1999/12/22/pubmed PY - 1999/12/22/medline PY - 1999/12/22/entrez SP - 185 EP - 93 JF - The Journal of surgical research JO - J Surg Res VL - 87 IS - 2 N2 - BACKGROUND: Motoneurons in the spinal cord are especially vulnerable to ischemic injury and selectively destroyed after transient ischemia. Nitric oxide (NO) has been implicated in both neurodegneration and neuroprotection to ischemic insult. To evaluate the role of NO in pathophysiology to spinal cord ischemia, the expression of neuronal and inducible nitric oxide synthase (n-NOS and i-NOS) and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) in the motoneurons of the lumbosacral spinal cord was examined in a rat model with transient abdominal aorta (TAA) occlusion. MATERIALS AND METHODS: Male Sprague-Dawley rats were divided into sham-operated (n = 12) and TAA occlusion (n = 24) groups. TAA occlusion was induced by placement of a microvascular clamp around the abdominal aorta for 20 min. Three sham-operated and six TAA occlusion animals were sacrificed at each time interval at 4, 24, and 48 h and 7 days after operation. Tissue sections obtained from the lumbosacral spinal cord were processed for n-NOS, i-NOS, NADPH-d, and hematoxylin-eosin (HE) staining. Histological changes of motoneurons in ventral horn were assessed by HE staining. RESULTS: In sham-operated control animals, n-NOS-, i-NOS-, and NADPH-d-positive neurons were barely detectable in the ventral horn of the spinal cord. At 4 h after TTA occlusion, n-NOS and NADPH-d expression became evident in the motoneurons and was markedly enhanced at 24 and 48 h. i-NOS expression was also induced in the ventral horn motoneurons of the lumbosacral spinal cord at the same time points. Enzymatic expression in the motoneurons was diminished 7 days after operation. Hyperchromatic neurons indicative of cell death were observed in HE-stained specimens 7 days following TAA occlusion. CONCLUSIONS: The rapid induction of n-NOS, i-NOS, and NADPH-d in the motoneurons of ventral horn suggests that NO may be involved in the selective and delayed neuronal death in the spinal cord to the ischemic insult. SN - 0022-4804 UR - https://www.unboundmedicine.com/medline/citation/10600348/Induction_of_neuronal_and_inducible_nitric_oxide_synthase_in_the_motoneurons_of_spinal_cord_following_transient_abdominal_aorta_occlusion_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(99)95754-2 DB - PRIME DP - Unbound Medicine ER -