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Screening for coeliac disease in healthy blood donors at two immuno-transfusion centres in north-east Italy.
Ital J Gastroenterol Hepatol 1999; 31(7):584-6IJ

Abstract

BACKGROUND AND AIMS

In the past, the reported prevalence of coeliac disease ranged from 1:1000 to 1:4000, whereas recent studies using serological screening methods have found a significantly higher prevalence. The aim of this study was to investigate the prevalence of coeliac disease in healthy blood donors in a North-eastern region of Italy.

SUBJECTS

A total of 4000 healthy blood donors were studied from two immunotransfusion centres.

METHODS

Serum IgA-antiendomysium antibodies were detected by indirect immunofluorescence using human umbilical cord vein sections, and positive sera were tested also on monkey oesophagus tissue. Intestinal biopsy was performed in all antiendomysium-positive subjects.

RESULTS

Ten out of 4000 sera screened were found to be antiendomysium positive on human umbilical cord vein. All positive patients had flat mucosa on intestinal biopsy. Five subjects had coeliac disease-related clinical features (2 had a history of gastrointestinal symptoms, 1 a family history of IDDM, 1 sideropenic anaemia, and 1 IgA deficiency). One of the ten serum, antiendomysium positive on human umbilical cord vein, was found to be negative when tested on monkey oesophagus.

CONCLUSIONS

These data confirm the high prevalence of undiagnosed silent coeliac disease in the healthy adult population. This is the first study where umbilical cord was used for screening coeliac disease in a large population. The human umbilical cord vein indirect immunofluorescence test is more specific for villous atrophy than conventional indirect immunofluorescence test on monkey oesophagus and is a reliable screening test for coeliac disease in an apparently healthy population.

Authors+Show Affiliations

Department of Paediatrics, IRCCS Burlo Garofolo, Trieste, Italy. chtrevi@tin.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10604097

Citation

Trevisiol, C, et al. "Screening for Coeliac Disease in Healthy Blood Donors at Two Immuno-transfusion Centres in North-east Italy." Italian Journal of Gastroenterology and Hepatology, vol. 31, no. 7, 1999, pp. 584-6.
Trevisiol C, Not T, Berti I, et al. Screening for coeliac disease in healthy blood donors at two immuno-transfusion centres in north-east Italy. Ital J Gastroenterol Hepatol. 1999;31(7):584-6.
Trevisiol, C., Not, T., Berti, I., Buratti, E., Città, A., Neri, E., ... Ventura, A. (1999). Screening for coeliac disease in healthy blood donors at two immuno-transfusion centres in north-east Italy. Italian Journal of Gastroenterology and Hepatology, 31(7), pp. 584-6.
Trevisiol C, et al. Screening for Coeliac Disease in Healthy Blood Donors at Two Immuno-transfusion Centres in North-east Italy. Ital J Gastroenterol Hepatol. 1999;31(7):584-6. PubMed PMID: 10604097.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screening for coeliac disease in healthy blood donors at two immuno-transfusion centres in north-east Italy. AU - Trevisiol,C, AU - Not,T, AU - Berti,I, AU - Buratti,E, AU - Città,A, AU - Neri,E, AU - Torre,G, AU - Martelossi,S, AU - Tommasini,A, AU - Alù,A, AU - Barillari,G, AU - Facchini,S, AU - Ventura,A, PY - 1999/12/22/pubmed PY - 1999/12/22/medline PY - 1999/12/22/entrez SP - 584 EP - 6 JF - Italian journal of gastroenterology and hepatology JO - Ital J Gastroenterol Hepatol VL - 31 IS - 7 N2 - BACKGROUND AND AIMS: In the past, the reported prevalence of coeliac disease ranged from 1:1000 to 1:4000, whereas recent studies using serological screening methods have found a significantly higher prevalence. The aim of this study was to investigate the prevalence of coeliac disease in healthy blood donors in a North-eastern region of Italy. SUBJECTS: A total of 4000 healthy blood donors were studied from two immunotransfusion centres. METHODS: Serum IgA-antiendomysium antibodies were detected by indirect immunofluorescence using human umbilical cord vein sections, and positive sera were tested also on monkey oesophagus tissue. Intestinal biopsy was performed in all antiendomysium-positive subjects. RESULTS: Ten out of 4000 sera screened were found to be antiendomysium positive on human umbilical cord vein. All positive patients had flat mucosa on intestinal biopsy. Five subjects had coeliac disease-related clinical features (2 had a history of gastrointestinal symptoms, 1 a family history of IDDM, 1 sideropenic anaemia, and 1 IgA deficiency). One of the ten serum, antiendomysium positive on human umbilical cord vein, was found to be negative when tested on monkey oesophagus. CONCLUSIONS: These data confirm the high prevalence of undiagnosed silent coeliac disease in the healthy adult population. This is the first study where umbilical cord was used for screening coeliac disease in a large population. The human umbilical cord vein indirect immunofluorescence test is more specific for villous atrophy than conventional indirect immunofluorescence test on monkey oesophagus and is a reliable screening test for coeliac disease in an apparently healthy population. SN - 1125-8055 UR - https://www.unboundmedicine.com/medline/citation/10604097/Screening_for_coeliac_disease_in_healthy_blood_donors_at_two_immuno_transfusion_centres_in_north_east_Italy_ L2 - https://medlineplus.gov/bloodtransfusionanddonation.html DB - PRIME DP - Unbound Medicine ER -