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HPLC with fluorescence detection of methamphetamine and amphetamine in segmentally analyzed human hair.
Analyst. 1999 Apr; 124(4):493-7.A

Abstract

A sensitive high-performance liquid chromatographic method with fluorescence detection for determining methamphetamine and its major metabolite, amphetamine, in abusers' hair segments was developed. Methamphetamine and amphetamine in hair samples collected from addicts were extracted into acidified methanol, derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride, separated isocratically on an ODS column using TRIS-HCl buffer (0.1 mol dm-3, pH 7.0)-methanol (30 + 70 v/v) as the mobile phase and the derivatives were detected fluorimetrically at 440 nm (lambda ex 330 nm). Calibration curves obtained by using control human hair spiked with standard solutions were linear (r > or = 0.999) up to at least 676.1 ng mg-1 for amphetamine and 746.1 ng mg-1 for methamphetamine. The detection limits at a signal-to-noise ratio of 3 were 51.4 and 74.6 pg mg-1 hair for amphetamine and methamphetamine, respectively. Using control hair spiked with standard solutions, the intra- and inter-day relative standard deviations (n = 5) were < or = 8.6% for both the target compounds. The method was successfully applied to the segmental analyses of methamphetamine abusers' hair samples.

Authors+Show Affiliations

School of Pharmaceutical Sciences, Nagasaki University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10605878

Citation

al-Dirbashi, O, et al. "HPLC With Fluorescence Detection of Methamphetamine and Amphetamine in Segmentally Analyzed Human Hair." The Analyst, vol. 124, no. 4, 1999, pp. 493-7.
al-Dirbashi O, Kuroda N, Inuduka S, et al. HPLC with fluorescence detection of methamphetamine and amphetamine in segmentally analyzed human hair. Analyst. 1999;124(4):493-7.
al-Dirbashi, O., Kuroda, N., Inuduka, S., Menichini, F., & Nakashima, K. (1999). HPLC with fluorescence detection of methamphetamine and amphetamine in segmentally analyzed human hair. The Analyst, 124(4), 493-7.
al-Dirbashi O, et al. HPLC With Fluorescence Detection of Methamphetamine and Amphetamine in Segmentally Analyzed Human Hair. Analyst. 1999;124(4):493-7. PubMed PMID: 10605878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPLC with fluorescence detection of methamphetamine and amphetamine in segmentally analyzed human hair. AU - al-Dirbashi,O, AU - Kuroda,N, AU - Inuduka,S, AU - Menichini,F, AU - Nakashima,K, PY - 1999/12/22/pubmed PY - 1999/12/22/medline PY - 1999/12/22/entrez SP - 493 EP - 7 JF - The Analyst JO - Analyst VL - 124 IS - 4 N2 - A sensitive high-performance liquid chromatographic method with fluorescence detection for determining methamphetamine and its major metabolite, amphetamine, in abusers' hair segments was developed. Methamphetamine and amphetamine in hair samples collected from addicts were extracted into acidified methanol, derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride, separated isocratically on an ODS column using TRIS-HCl buffer (0.1 mol dm-3, pH 7.0)-methanol (30 + 70 v/v) as the mobile phase and the derivatives were detected fluorimetrically at 440 nm (lambda ex 330 nm). Calibration curves obtained by using control human hair spiked with standard solutions were linear (r > or = 0.999) up to at least 676.1 ng mg-1 for amphetamine and 746.1 ng mg-1 for methamphetamine. The detection limits at a signal-to-noise ratio of 3 were 51.4 and 74.6 pg mg-1 hair for amphetamine and methamphetamine, respectively. Using control hair spiked with standard solutions, the intra- and inter-day relative standard deviations (n = 5) were < or = 8.6% for both the target compounds. The method was successfully applied to the segmental analyses of methamphetamine abusers' hair samples. SN - 0003-2654 UR - https://www.unboundmedicine.com/medline/citation/10605878/HPLC_with_fluorescence_detection_of_methamphetamine_and_amphetamine_in_segmentally_analyzed_human_hair_ L2 - https://doi.org/10.1039/a808912d DB - PRIME DP - Unbound Medicine ER -