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Human Tc1 and Tc2/Tc0 CD8 T-cell clones display distinct cell surface and functional phenotypes.
Blood. 2000 Jan 01; 95(1):231-40.Blood

Abstract

It has recently become clear that distinct subsets of CD8 T cells, analogous to their CD4 counterparts, exist in rodents and humans. To examine functional differences between human CD8 T-cell subsets, we generated Tc1, Tc2, and Tc0 T-cell clones from the peripheral blood of healthy individuals. The majority of CD8 T-cell clones generated displayed a classic Tc1 phenotype, but 10% to 20% secreted interleukin (IL)-4 in addition to interferon-gamma (Tc0 phenotype). Generation of Tc2 clones was dependent on the use of anti-CD3 and anti-CD28 as the primary stimulus. The cytokine profiles of established clones remained susceptible to modification by the addition of IL-12 and IL-4. In addition, IL-12 enhanced and IL-4 inhibited the growth of Tc1 but not Tc2/0 CD8 T-cell clones. Significant functional differences were observed between the subsets. Tc2/0 clones expressed CD30 and CD40 ligand at a much higher level than Tc1 clones. Both Tc1 and Tc2/0 clones showed comparable cytotoxicity and produced similar levels of perforin and Fas L. However, Tc2 clones were much more resistant to activation-induced cell death and less susceptible to apoptosis by direct Fas ligation. Moreover, Tc1 and Tc2 clones had opposing effects on the development of CD4 effectors, promoting type 1 and type 2 responses, respectively. These data provide evidence for profound differences between human CD8 T-cell subsets that may be important in their functions as cytotoxic or immunoregulatory cells. (Blood. 2000;95:231-240)

Authors+Show Affiliations

Department of Immunology, Guy's, King's, and St. Thomas' School of Medicine, Rayne Institute, London, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10607707

Citation

Vukmanovic-Stejic, M, et al. "Human Tc1 and Tc2/Tc0 CD8 T-cell Clones Display Distinct Cell Surface and Functional Phenotypes." Blood, vol. 95, no. 1, 2000, pp. 231-40.
Vukmanovic-Stejic M, Vyas B, Gorak-Stolinska P, et al. Human Tc1 and Tc2/Tc0 CD8 T-cell clones display distinct cell surface and functional phenotypes. Blood. 2000;95(1):231-40.
Vukmanovic-Stejic, M., Vyas, B., Gorak-Stolinska, P., Noble, A., & Kemeny, D. M. (2000). Human Tc1 and Tc2/Tc0 CD8 T-cell clones display distinct cell surface and functional phenotypes. Blood, 95(1), 231-40.
Vukmanovic-Stejic M, et al. Human Tc1 and Tc2/Tc0 CD8 T-cell Clones Display Distinct Cell Surface and Functional Phenotypes. Blood. 2000 Jan 1;95(1):231-40. PubMed PMID: 10607707.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human Tc1 and Tc2/Tc0 CD8 T-cell clones display distinct cell surface and functional phenotypes. AU - Vukmanovic-Stejic,M, AU - Vyas,B, AU - Gorak-Stolinska,P, AU - Noble,A, AU - Kemeny,D M, PY - 1999/12/23/pubmed PY - 1999/12/23/medline PY - 1999/12/23/entrez SP - 231 EP - 40 JF - Blood JO - Blood VL - 95 IS - 1 N2 - It has recently become clear that distinct subsets of CD8 T cells, analogous to their CD4 counterparts, exist in rodents and humans. To examine functional differences between human CD8 T-cell subsets, we generated Tc1, Tc2, and Tc0 T-cell clones from the peripheral blood of healthy individuals. The majority of CD8 T-cell clones generated displayed a classic Tc1 phenotype, but 10% to 20% secreted interleukin (IL)-4 in addition to interferon-gamma (Tc0 phenotype). Generation of Tc2 clones was dependent on the use of anti-CD3 and anti-CD28 as the primary stimulus. The cytokine profiles of established clones remained susceptible to modification by the addition of IL-12 and IL-4. In addition, IL-12 enhanced and IL-4 inhibited the growth of Tc1 but not Tc2/0 CD8 T-cell clones. Significant functional differences were observed between the subsets. Tc2/0 clones expressed CD30 and CD40 ligand at a much higher level than Tc1 clones. Both Tc1 and Tc2/0 clones showed comparable cytotoxicity and produced similar levels of perforin and Fas L. However, Tc2 clones were much more resistant to activation-induced cell death and less susceptible to apoptosis by direct Fas ligation. Moreover, Tc1 and Tc2 clones had opposing effects on the development of CD4 effectors, promoting type 1 and type 2 responses, respectively. These data provide evidence for profound differences between human CD8 T-cell subsets that may be important in their functions as cytotoxic or immunoregulatory cells. (Blood. 2000;95:231-240) SN - 0006-4971 UR - https://www.unboundmedicine.com/medline/citation/10607707/Human_Tc1_and_Tc2/Tc0_CD8_T_cell_clones_display_distinct_cell_surface_and_functional_phenotypes_ L2 - https://ashpublications.org/blood/article-lookup/doi/&lo.doi; DB - PRIME DP - Unbound Medicine ER -