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Enantiomer-specific high-performance liquid chromatography with fluorescence detection of methamphetamines in abusers' hair and urine.
Biomed Chromatogr. 1999 Dec; 13(8):543-7.BC

Abstract

Enantiomer-specific high-performance liquid chromatography with fluorescence detection using 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride as a fluorescence labeling reagent was applied to determine methamphetamine and its metabolites in abusers' hair and urine. Hair samples were segmentally analyzed based on 1 cm long segments. In four hair samples, only the S(+)-enantiomers of methamphetamine and its N-demethylated metabolite, S(+)-amphetamine were detected. Satisfactory correlation (r = 0.901) between the results of high-performance liquid chromatography-fluorescence and those of gas chromatography-nitrogen phosphorous detection was obtained (n = 19). In an abuser's urine sample, the S(+)- and R(-)-enantiomers of methamphetamine, amphetamine and para-hydroxymethamphetamine were detected. The degree of N-demethylation of S(+)-methamphetamine into the corresponding metabolite of amphetamine was significantly higher than that of the R(-)-enantiomer.

Authors+Show Affiliations

School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

10611609

Citation

Al-Dirbashi, O, et al. "Enantiomer-specific High-performance Liquid Chromatography With Fluorescence Detection of Methamphetamines in Abusers' Hair and Urine." Biomedical Chromatography : BMC, vol. 13, no. 8, 1999, pp. 543-7.
Al-Dirbashi O, Wada M, Kuroda N, et al. Enantiomer-specific high-performance liquid chromatography with fluorescence detection of methamphetamines in abusers' hair and urine. Biomed Chromatogr. 1999;13(8):543-7.
Al-Dirbashi, O., Wada, M., Kuroda, N., Inuduka, S., & Nakashima, K. (1999). Enantiomer-specific high-performance liquid chromatography with fluorescence detection of methamphetamines in abusers' hair and urine. Biomedical Chromatography : BMC, 13(8), 543-7.
Al-Dirbashi O, et al. Enantiomer-specific High-performance Liquid Chromatography With Fluorescence Detection of Methamphetamines in Abusers' Hair and Urine. Biomed Chromatogr. 1999;13(8):543-7. PubMed PMID: 10611609.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enantiomer-specific high-performance liquid chromatography with fluorescence detection of methamphetamines in abusers' hair and urine. AU - Al-Dirbashi,O, AU - Wada,M, AU - Kuroda,N, AU - Inuduka,S, AU - Nakashima,K, PY - 1999/12/28/pubmed PY - 1999/12/28/medline PY - 1999/12/28/entrez SP - 543 EP - 7 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 13 IS - 8 N2 - Enantiomer-specific high-performance liquid chromatography with fluorescence detection using 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride as a fluorescence labeling reagent was applied to determine methamphetamine and its metabolites in abusers' hair and urine. Hair samples were segmentally analyzed based on 1 cm long segments. In four hair samples, only the S(+)-enantiomers of methamphetamine and its N-demethylated metabolite, S(+)-amphetamine were detected. Satisfactory correlation (r = 0.901) between the results of high-performance liquid chromatography-fluorescence and those of gas chromatography-nitrogen phosphorous detection was obtained (n = 19). In an abuser's urine sample, the S(+)- and R(-)-enantiomers of methamphetamine, amphetamine and para-hydroxymethamphetamine were detected. The degree of N-demethylation of S(+)-methamphetamine into the corresponding metabolite of amphetamine was significantly higher than that of the R(-)-enantiomer. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/10611609/Enantiomer_specific_high_performance_liquid_chromatography_with_fluorescence_detection_of_methamphetamines_in_abusers'_hair_and_urine_ L2 - https://doi.org/10.1002/(SICI)1099-0801(199912)13:8<543::AID-BMC925>3.0.CO;2-7 DB - PRIME DP - Unbound Medicine ER -