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Stimulation of hyphal growth in anaerobic cultures of Mucor rouxii by extracellular trehalose. Relevance of cell wall-bound activity of acid trehalase for trehalose utilization.
FEMS Microbiol Lett. 2000 Jan 01; 182(1):9-13.FM

Abstract

In fungi, the hydrolysis of extracellular trehalose is carried out by acid trehalases. These secretory glycoproteins may be more abundant either in the vacuolar compartment, like in yeast, or at the cell surface, such as in many filamentous fungi. The relative efficiency of these two compartments for the utilization of extracellular trehalose was investigated using as a model the dimorphic fungus Mucor rouxii, which produces yeast-like cells under a CO(2) atmosphere, or hyphae in the presence of air. Under CO(2), cultures supplemented with glucose produced yeast-like cells devoid of acid trehalase activity. On the other hand, trehalose-supplemented cultures developed hyphae exhibiting cell wall-bound and intracellular acid trehalase activity. Glucose-grown yeast-like cells supplemented with trehalose after glucose exhaustion, induced intracellular activity of acid trehalase, but no activity was detected at the cell surface. Even endowed of significant intracellular activity of acid trehalase, these cells did not grow further. When exposed to air these yeast-like produced germ tubes exhibiting cell wall-bound acid trehalase activity. These results suggest that the utilization of extracellular trehalose as a source of carbon for growth requires the localization of acid trehalase activity at the cell surface. Our results also show that extracellular trehalose elicits a morphogenetic phenomenon, inducing the formation of hyphae which are the physiological support for acid trehalase activity.

Authors+Show Affiliations

Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, 14.040-901 Ribeirão Preto, São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10612723

Citation

Lucio, A K., et al. "Stimulation of Hyphal Growth in Anaerobic Cultures of Mucor Rouxii By Extracellular Trehalose. Relevance of Cell Wall-bound Activity of Acid Trehalase for Trehalose Utilization." FEMS Microbiology Letters, vol. 182, no. 1, 2000, pp. 9-13.
Lucio AK, Polizeli ML, Jorge JA, et al. Stimulation of hyphal growth in anaerobic cultures of Mucor rouxii by extracellular trehalose. Relevance of cell wall-bound activity of acid trehalase for trehalose utilization. FEMS Microbiol Lett. 2000;182(1):9-13.
Lucio, A. K., Polizeli, M. L., Jorge, J. A., & Terenzi, H. F. (2000). Stimulation of hyphal growth in anaerobic cultures of Mucor rouxii by extracellular trehalose. Relevance of cell wall-bound activity of acid trehalase for trehalose utilization. FEMS Microbiology Letters, 182(1), 9-13.
Lucio AK, et al. Stimulation of Hyphal Growth in Anaerobic Cultures of Mucor Rouxii By Extracellular Trehalose. Relevance of Cell Wall-bound Activity of Acid Trehalase for Trehalose Utilization. FEMS Microbiol Lett. 2000 Jan 1;182(1):9-13. PubMed PMID: 10612723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stimulation of hyphal growth in anaerobic cultures of Mucor rouxii by extracellular trehalose. Relevance of cell wall-bound activity of acid trehalase for trehalose utilization. AU - Lucio,A K, AU - Polizeli,M L, AU - Jorge,J A, AU - Terenzi,H F, PY - 1999/12/29/pubmed PY - 2000/3/25/medline PY - 1999/12/29/entrez SP - 9 EP - 13 JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 182 IS - 1 N2 - In fungi, the hydrolysis of extracellular trehalose is carried out by acid trehalases. These secretory glycoproteins may be more abundant either in the vacuolar compartment, like in yeast, or at the cell surface, such as in many filamentous fungi. The relative efficiency of these two compartments for the utilization of extracellular trehalose was investigated using as a model the dimorphic fungus Mucor rouxii, which produces yeast-like cells under a CO(2) atmosphere, or hyphae in the presence of air. Under CO(2), cultures supplemented with glucose produced yeast-like cells devoid of acid trehalase activity. On the other hand, trehalose-supplemented cultures developed hyphae exhibiting cell wall-bound and intracellular acid trehalase activity. Glucose-grown yeast-like cells supplemented with trehalose after glucose exhaustion, induced intracellular activity of acid trehalase, but no activity was detected at the cell surface. Even endowed of significant intracellular activity of acid trehalase, these cells did not grow further. When exposed to air these yeast-like produced germ tubes exhibiting cell wall-bound acid trehalase activity. These results suggest that the utilization of extracellular trehalose as a source of carbon for growth requires the localization of acid trehalase activity at the cell surface. Our results also show that extracellular trehalose elicits a morphogenetic phenomenon, inducing the formation of hyphae which are the physiological support for acid trehalase activity. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/10612723/Stimulation_of_hyphal_growth_in_anaerobic_cultures_of_Mucor_rouxii_by_extracellular_trehalose__Relevance_of_cell_wall_bound_activity_of_acid_trehalase_for_trehalose_utilization_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.2000.tb08865.x DB - PRIME DP - Unbound Medicine ER -