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High performance liquid chromatography/electrospray mass spectrometry of Hypericum perforatum extracts.
Rapid Commun Mass Spectrom. 2000; 14(2):95-9.RC

Abstract

Hypericum perforatum L. (St. John's Wort) is a widely distributed herbaceous perennial plant which has been well known as a medicinal plant since antiquity. In recent years, H. perforatum has received increasing attention for the treatment of depression and other neuralgic disorders. The main constituents of H. perforatum extract include flavonoids, naphthodianthrones, phloroglucinols, essential oils and xanthones. The present work reports the analysis of naphthodianthrones and phloroglucinols in H. perforatum extracts by means of high performance liquid chromatography (HPLC) coupled simultaneously to a diode array detector (DAD) and electrospray mass spectrometry (ESI-MS). Hypericin, pseudohypericin, hyperforin and adhyperforin were separated and identified on the base of their on-line UV and mass spectra. Quantitative analysis of hypericin derivatives in different extracts of H. perforatum using DAD and MS detectors was performed. In addition, direct infusion ESI-MS of H. perforatum extracts was applied to obtain rapid mass fingerprints of constituents present in the sample.

Authors+Show Affiliations

ITBA/CNR, Via Fratelli Cervi, 93-20090 Segrate (Milano), Italy. mauri@itba.mi.cnr.itNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10623936

Citation

Mauri, P, and P Pietta. "High Performance Liquid Chromatography/electrospray Mass Spectrometry of Hypericum Perforatum Extracts." Rapid Communications in Mass Spectrometry : RCM, vol. 14, no. 2, 2000, pp. 95-9.
Mauri P, Pietta P. High performance liquid chromatography/electrospray mass spectrometry of Hypericum perforatum extracts. Rapid Commun Mass Spectrom. 2000;14(2):95-9.
Mauri, P., & Pietta, P. (2000). High performance liquid chromatography/electrospray mass spectrometry of Hypericum perforatum extracts. Rapid Communications in Mass Spectrometry : RCM, 14(2), 95-9.
Mauri P, Pietta P. High Performance Liquid Chromatography/electrospray Mass Spectrometry of Hypericum Perforatum Extracts. Rapid Commun Mass Spectrom. 2000;14(2):95-9. PubMed PMID: 10623936.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High performance liquid chromatography/electrospray mass spectrometry of Hypericum perforatum extracts. AU - Mauri,P, AU - Pietta,P, PY - 2000/1/7/pubmed PY - 2000/2/19/medline PY - 2000/1/7/entrez SP - 95 EP - 9 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 14 IS - 2 N2 - Hypericum perforatum L. (St. John's Wort) is a widely distributed herbaceous perennial plant which has been well known as a medicinal plant since antiquity. In recent years, H. perforatum has received increasing attention for the treatment of depression and other neuralgic disorders. The main constituents of H. perforatum extract include flavonoids, naphthodianthrones, phloroglucinols, essential oils and xanthones. The present work reports the analysis of naphthodianthrones and phloroglucinols in H. perforatum extracts by means of high performance liquid chromatography (HPLC) coupled simultaneously to a diode array detector (DAD) and electrospray mass spectrometry (ESI-MS). Hypericin, pseudohypericin, hyperforin and adhyperforin were separated and identified on the base of their on-line UV and mass spectra. Quantitative analysis of hypericin derivatives in different extracts of H. perforatum using DAD and MS detectors was performed. In addition, direct infusion ESI-MS of H. perforatum extracts was applied to obtain rapid mass fingerprints of constituents present in the sample. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/10623936/High_performance_liquid_chromatography/electrospray_mass_spectrometry_of_Hypericum_perforatum_extracts_ L2 - https://doi.org/10.1002/(SICI)1097-0231(20000130)14:2<95::AID-RCM843>3.0.CO;2-6 DB - PRIME DP - Unbound Medicine ER -