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NeSL-1, an ancient lineage of site-specific non-LTR retrotransposons from Caenorhabditis elegans.
Genetics. 2000 Jan; 154(1):193-203.G

Abstract

Phylogenetic analyses of non-LTR retrotransposons suggest that all elements can be divided into 11 lineages. The 3 oldest lineages show target site specificity for unique locations in the genome and encode an endonuclease with an active site similar to certain restriction enzymes. The more "modern" non-LTR lineages possess an apurinic endonuclease-like domain and generally lack site specificity. The genome sequence of Caenorhabditis elegans reveals the presence of a non-LTR retrotransposon that resembles the older elements, in that it contains a single open reading frame with a carboxyl-terminal restriction-like endonuclease domain. Located near the N-terminal end of the ORF is a cysteine protease domain not found in any other non-LTR element. The N2 strain of C. elegans appears to contain only one full-length and several 5' truncated copies of this element. The elements specifically insert in the Spliced leader-1 genes; hence the element has been named NeSL-1 (Nematode Spliced Leader-1). Phylogenetic analysis confirms that NeSL-1 branches very early in the non-LTR lineage and that it represents a 12th lineage of non-LTR elements. The target specificity of NeSL-1 for the spliced leader exons and the similarity of its structure to that of R2 elements leads to a simple model for its expression and retrotransposition.

Authors+Show Affiliations

Department of Biology, University of Rochester, Rochester, New York 14627-0211, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10628980

Citation

Malik, H S., and T H. Eickbush. "NeSL-1, an Ancient Lineage of Site-specific non-LTR Retrotransposons From Caenorhabditis Elegans." Genetics, vol. 154, no. 1, 2000, pp. 193-203.
Malik HS, Eickbush TH. NeSL-1, an ancient lineage of site-specific non-LTR retrotransposons from Caenorhabditis elegans. Genetics. 2000;154(1):193-203.
Malik, H. S., & Eickbush, T. H. (2000). NeSL-1, an ancient lineage of site-specific non-LTR retrotransposons from Caenorhabditis elegans. Genetics, 154(1), 193-203.
Malik HS, Eickbush TH. NeSL-1, an Ancient Lineage of Site-specific non-LTR Retrotransposons From Caenorhabditis Elegans. Genetics. 2000;154(1):193-203. PubMed PMID: 10628980.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NeSL-1, an ancient lineage of site-specific non-LTR retrotransposons from Caenorhabditis elegans. AU - Malik,H S, AU - Eickbush,T H, PY - 2000/1/11/pubmed PY - 2000/2/19/medline PY - 2000/1/11/entrez SP - 193 EP - 203 JF - Genetics JO - Genetics VL - 154 IS - 1 N2 - Phylogenetic analyses of non-LTR retrotransposons suggest that all elements can be divided into 11 lineages. The 3 oldest lineages show target site specificity for unique locations in the genome and encode an endonuclease with an active site similar to certain restriction enzymes. The more "modern" non-LTR lineages possess an apurinic endonuclease-like domain and generally lack site specificity. The genome sequence of Caenorhabditis elegans reveals the presence of a non-LTR retrotransposon that resembles the older elements, in that it contains a single open reading frame with a carboxyl-terminal restriction-like endonuclease domain. Located near the N-terminal end of the ORF is a cysteine protease domain not found in any other non-LTR element. The N2 strain of C. elegans appears to contain only one full-length and several 5' truncated copies of this element. The elements specifically insert in the Spliced leader-1 genes; hence the element has been named NeSL-1 (Nematode Spliced Leader-1). Phylogenetic analysis confirms that NeSL-1 branches very early in the non-LTR lineage and that it represents a 12th lineage of non-LTR elements. The target specificity of NeSL-1 for the spliced leader exons and the similarity of its structure to that of R2 elements leads to a simple model for its expression and retrotransposition. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/10628980/NeSL_1_an_ancient_lineage_of_site_specific_non_LTR_retrotransposons_from_Caenorhabditis_elegans_ L2 - http://www.genetics.org/cgi/pmidlookup?view=long&pmid=10628980 DB - PRIME DP - Unbound Medicine ER -