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Mutational analysis of differences in thermostability between histones from mesophilic and hyperthermophilic archaea.
J Bacteriol. 2000 Feb; 182(3):812-7.JB

Abstract

Amino acid residues responsible for the large difference in thermostability between HMfB and HFoB, archaeal histones from the hyperthermophile Methanothermus fervidus and the mesophile Methanobacterium formicicum, respectively, have been identified by site-specific mutagenesis. The thermal denaturation of approximately 70 archaeal histone variants has been monitored by circular dichroism, and the data generated were fit to a two-state unfolding model (dimer-->two random coil monomers) to obtain a standard-state (1M) melting temperature for each variant dimer. The results of single-, double-, and triple-residue substitutions reveal that the much higher stability of rHMfB dimers, relative to rHFoB dimers, is conferred predominantly by improved intermolecular hydrophobic interactions near the center of the histone dimer core and by additional favorable ion pairs on the dimer surface.

Authors+Show Affiliations

Department of Microbiology, The Ohio State University, Columbus, Ohio 43210, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10633118

Citation

Li, W T., et al. "Mutational Analysis of Differences in Thermostability Between Histones From Mesophilic and Hyperthermophilic Archaea." Journal of Bacteriology, vol. 182, no. 3, 2000, pp. 812-7.
Li WT, Shriver JW, Reeve JN. Mutational analysis of differences in thermostability between histones from mesophilic and hyperthermophilic archaea. J Bacteriol. 2000;182(3):812-7.
Li, W. T., Shriver, J. W., & Reeve, J. N. (2000). Mutational analysis of differences in thermostability between histones from mesophilic and hyperthermophilic archaea. Journal of Bacteriology, 182(3), 812-7.
Li WT, Shriver JW, Reeve JN. Mutational Analysis of Differences in Thermostability Between Histones From Mesophilic and Hyperthermophilic Archaea. J Bacteriol. 2000;182(3):812-7. PubMed PMID: 10633118.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutational analysis of differences in thermostability between histones from mesophilic and hyperthermophilic archaea. AU - Li,W T, AU - Shriver,J W, AU - Reeve,J N, PY - 2000/1/14/pubmed PY - 2000/1/14/medline PY - 2000/1/14/entrez SP - 812 EP - 7 JF - Journal of bacteriology JO - J Bacteriol VL - 182 IS - 3 N2 - Amino acid residues responsible for the large difference in thermostability between HMfB and HFoB, archaeal histones from the hyperthermophile Methanothermus fervidus and the mesophile Methanobacterium formicicum, respectively, have been identified by site-specific mutagenesis. The thermal denaturation of approximately 70 archaeal histone variants has been monitored by circular dichroism, and the data generated were fit to a two-state unfolding model (dimer-->two random coil monomers) to obtain a standard-state (1M) melting temperature for each variant dimer. The results of single-, double-, and triple-residue substitutions reveal that the much higher stability of rHMfB dimers, relative to rHFoB dimers, is conferred predominantly by improved intermolecular hydrophobic interactions near the center of the histone dimer core and by additional favorable ion pairs on the dimer surface. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/10633118/Mutational_analysis_of_differences_in_thermostability_between_histones_from_mesophilic_and_hyperthermophilic_archaea_ L2 - https://journals.asm.org/doi/10.1128/JB.182.3.812-817.2000?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -