Tags

Type your tag names separated by a space and hit enter

Determination of pentazocine in human whole blood and urine by gas chromatography/surface ionization organic mass spectrometry.
J Mass Spectrom. 2000 Jan; 35(1):33-8.JM

Abstract

Pentazocine has been found to be measurable with much higher sensitivity by gas chromatography (GC)/surface ionization (SI) organic mass spectrometry (OMS) than by the conventional GC/electron ionization (EI) mass spectrometry. The compound was extracted from human whole blood and urine with Sep-Pak C(18) cartridges before analysis by GC/SIOMS; recoveries were > 96.6% for both samples. The calibration curves were linear in the range 6.25-100 ng ml(-1) and the detection limits were 500 pg ml(-1) of a sample by selected ion monitoring (SIM) with GC/SIOMS. The intra- and inter-day relative standard deviations for the determination of pentazocine in whole blood and urine were not greater than 9.6%. The sensitivity for pentazocine obtained by SI-SIM was about 60 times higher than that obtained by EI-SIM. To validate the present GC/SIOMS method for pentazocine, whole blood and urine samples collected from two volunteers 1-6 h after intramuscular injection of 15 mg of pentazocine were analyzed. The concentrations were 13.5-59.3 ng ml(-1) for whole blood and 0.39-4.00 microg ml(-1) for urine.

Authors+Show Affiliations

Department of Legal Medicine Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu 431-3192, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

10633232

Citation

Seno, H, et al. "Determination of Pentazocine in Human Whole Blood and Urine By Gas Chromatography/surface Ionization Organic Mass Spectrometry." Journal of Mass Spectrometry : JMS, vol. 35, no. 1, 2000, pp. 33-8.
Seno H, Kumazawa T, Ishii A, et al. Determination of pentazocine in human whole blood and urine by gas chromatography/surface ionization organic mass spectrometry. J Mass Spectrom. 2000;35(1):33-8.
Seno, H., Kumazawa, T., Ishii, A., Matsushima, H., Watanabe-Suzuki, K., & Suzuki, O. (2000). Determination of pentazocine in human whole blood and urine by gas chromatography/surface ionization organic mass spectrometry. Journal of Mass Spectrometry : JMS, 35(1), 33-8.
Seno H, et al. Determination of Pentazocine in Human Whole Blood and Urine By Gas Chromatography/surface Ionization Organic Mass Spectrometry. J Mass Spectrom. 2000;35(1):33-8. PubMed PMID: 10633232.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of pentazocine in human whole blood and urine by gas chromatography/surface ionization organic mass spectrometry. AU - Seno,H, AU - Kumazawa,T, AU - Ishii,A, AU - Matsushima,H, AU - Watanabe-Suzuki,K, AU - Suzuki,O, PY - 2000/1/14/pubmed PY - 2000/2/26/medline PY - 2000/1/14/entrez SP - 33 EP - 8 JF - Journal of mass spectrometry : JMS JO - J Mass Spectrom VL - 35 IS - 1 N2 - Pentazocine has been found to be measurable with much higher sensitivity by gas chromatography (GC)/surface ionization (SI) organic mass spectrometry (OMS) than by the conventional GC/electron ionization (EI) mass spectrometry. The compound was extracted from human whole blood and urine with Sep-Pak C(18) cartridges before analysis by GC/SIOMS; recoveries were > 96.6% for both samples. The calibration curves were linear in the range 6.25-100 ng ml(-1) and the detection limits were 500 pg ml(-1) of a sample by selected ion monitoring (SIM) with GC/SIOMS. The intra- and inter-day relative standard deviations for the determination of pentazocine in whole blood and urine were not greater than 9.6%. The sensitivity for pentazocine obtained by SI-SIM was about 60 times higher than that obtained by EI-SIM. To validate the present GC/SIOMS method for pentazocine, whole blood and urine samples collected from two volunteers 1-6 h after intramuscular injection of 15 mg of pentazocine were analyzed. The concentrations were 13.5-59.3 ng ml(-1) for whole blood and 0.39-4.00 microg ml(-1) for urine. SN - 1076-5174 UR - https://www.unboundmedicine.com/medline/citation/10633232/Determination_of_pentazocine_in_human_whole_blood_and_urine_by_gas_chromatography/surface_ionization_organic_mass_spectrometry_ L2 - https://doi.org/10.1002/(SICI)1096-9888(200001)35:1<33::AID-JMS909>3.0.CO;2-T DB - PRIME DP - Unbound Medicine ER -