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The N-terminal domain of human TAFII68 displays transactivation and oncogenic properties.
Oncogene. 1999 Dec 23; 18(56):8000-10.O

Abstract

In Ewing tumor, the (11;22) chromosomal translocation produces a chimeric molecule composed of the amino-terminal domain of EWS fused to the carboxyl-terminal DNA-binding domain of FLI-1. Previously, we have identified a novel protein TAFII68, which is highly similar to EWS and another closely related protein TLS (also called FUS). We demonstrate that the N-terminus of TAFII68 efficiently stimulates transcription when fused to two different DNA binding domains and that overexpression of TAFII68-FLI-1 chimeras in NIH3T3 cells leads to oncogenic transformation. We have also investigated the molecular mechanisms which could account for the transcriptional activation and the oncogenic transformation potential of the N-termini of TAFII68 and EWS. Thus, we have tested whether the artificial recruitment of components of the preinitiation complex (PIC) or a histone acetyltransferase (HAT) could bypass the requirement for the activation domains of either EWS or TAFII68. Recruitment of individual components of the transcription machinery or the GCN5 HAT is not sufficient to promote activation from FLI-1 responsive genes either in transfection experiments or in oncogenic transformation assays. These results suggest that the TAFII68 or EWS activation domains enhance a step after PIC formation in the transcriptional activation process.

Authors+Show Affiliations

Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, BP 163 - 67404 Illkirch Cedex, CU de Strasbourg, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10637511

Citation

Bertolotti, A, et al. "The N-terminal Domain of Human TAFII68 Displays Transactivation and Oncogenic Properties." Oncogene, vol. 18, no. 56, 1999, pp. 8000-10.
Bertolotti A, Bell B, Tora L. The N-terminal domain of human TAFII68 displays transactivation and oncogenic properties. Oncogene. 1999;18(56):8000-10.
Bertolotti, A., Bell, B., & Tora, L. (1999). The N-terminal domain of human TAFII68 displays transactivation and oncogenic properties. Oncogene, 18(56), 8000-10.
Bertolotti A, Bell B, Tora L. The N-terminal Domain of Human TAFII68 Displays Transactivation and Oncogenic Properties. Oncogene. 1999 Dec 23;18(56):8000-10. PubMed PMID: 10637511.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The N-terminal domain of human TAFII68 displays transactivation and oncogenic properties. AU - Bertolotti,A, AU - Bell,B, AU - Tora,L, PY - 2000/1/19/pubmed PY - 2000/1/19/medline PY - 2000/1/19/entrez SP - 8000 EP - 10 JF - Oncogene JO - Oncogene VL - 18 IS - 56 N2 - In Ewing tumor, the (11;22) chromosomal translocation produces a chimeric molecule composed of the amino-terminal domain of EWS fused to the carboxyl-terminal DNA-binding domain of FLI-1. Previously, we have identified a novel protein TAFII68, which is highly similar to EWS and another closely related protein TLS (also called FUS). We demonstrate that the N-terminus of TAFII68 efficiently stimulates transcription when fused to two different DNA binding domains and that overexpression of TAFII68-FLI-1 chimeras in NIH3T3 cells leads to oncogenic transformation. We have also investigated the molecular mechanisms which could account for the transcriptional activation and the oncogenic transformation potential of the N-termini of TAFII68 and EWS. Thus, we have tested whether the artificial recruitment of components of the preinitiation complex (PIC) or a histone acetyltransferase (HAT) could bypass the requirement for the activation domains of either EWS or TAFII68. Recruitment of individual components of the transcription machinery or the GCN5 HAT is not sufficient to promote activation from FLI-1 responsive genes either in transfection experiments or in oncogenic transformation assays. These results suggest that the TAFII68 or EWS activation domains enhance a step after PIC formation in the transcriptional activation process. SN - 0950-9232 UR - https://www.unboundmedicine.com/medline/citation/10637511/The_N_terminal_domain_of_human_TAFII68_displays_transactivation_and_oncogenic_properties_ L2 - https://doi.org/10.1038/sj.onc.1203207 DB - PRIME DP - Unbound Medicine ER -