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Preferential down-regulation of phospholipase C-beta in Ewing's sarcoma cells transfected with antisense EWS-Fli-1.
Br J Cancer. 2000 Jan; 82(1):16-9.BJ

Abstract

EWS-Fli-1, a fusion gene found in Ewing's sarcoma and primitive neuro-ectodermal tumour (PNET), encodes a transcriptional activator and promotes cellular transformation. We have made stable Ewing's sarcoma cells expressing antisense EWS-Fli-1 transcripts by transfecting the antisense EWS-Fli-1 expression plasmid. These cells showed partial loss of endogenous EWS-Fli-1 proteins and suppression of the cell growth. To elucidate the molecular mechanisms underlying the growth inhibition, we examined the changes of signal transducing proteins by immunoblot analysis in Ewing's sarcoma cells stably expressing antisense EWS-Fli-1 transcripts. Western blotting of the cell proteins revealed that expressions of phospholipase Cbeta2 and beta3 (PLCbeta2, PLCbeta3), and also protein kinase C alpha and beta (PKCalpha, beta) were significantly reduced by transfecting with antisense EWS-Fli-1. The inositol phosphates production by bradykinin (BK), but not platelet-derived growth factor (PDGF), was suppressed in these cells. These results suggest that the PLCbeta2 and PLCbeta3 may play a role in tumour proliferation in Ewing's sarcoma cells.

Authors+Show Affiliations

Department of Orthopaedic Surgery, Gifu University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10638960

Citation

Dohjima, T, et al. "Preferential Down-regulation of Phospholipase C-beta in Ewing's Sarcoma Cells Transfected With Antisense EWS-Fli-1." British Journal of Cancer, vol. 82, no. 1, 2000, pp. 16-9.
Dohjima T, Ohno T, Banno Y, et al. Preferential down-regulation of phospholipase C-beta in Ewing's sarcoma cells transfected with antisense EWS-Fli-1. Br J Cancer. 2000;82(1):16-9.
Dohjima, T., Ohno, T., Banno, Y., Nozawa, Y., Wen-yi, Y., & Shimizu, K. (2000). Preferential down-regulation of phospholipase C-beta in Ewing's sarcoma cells transfected with antisense EWS-Fli-1. British Journal of Cancer, 82(1), 16-9.
Dohjima T, et al. Preferential Down-regulation of Phospholipase C-beta in Ewing's Sarcoma Cells Transfected With Antisense EWS-Fli-1. Br J Cancer. 2000;82(1):16-9. PubMed PMID: 10638960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preferential down-regulation of phospholipase C-beta in Ewing's sarcoma cells transfected with antisense EWS-Fli-1. AU - Dohjima,T, AU - Ohno,T, AU - Banno,Y, AU - Nozawa,Y, AU - Wen-yi,Y, AU - Shimizu,K, PY - 2000/1/19/pubmed PY - 2000/1/19/medline PY - 2000/1/19/entrez SP - 16 EP - 9 JF - British journal of cancer JO - Br J Cancer VL - 82 IS - 1 N2 - EWS-Fli-1, a fusion gene found in Ewing's sarcoma and primitive neuro-ectodermal tumour (PNET), encodes a transcriptional activator and promotes cellular transformation. We have made stable Ewing's sarcoma cells expressing antisense EWS-Fli-1 transcripts by transfecting the antisense EWS-Fli-1 expression plasmid. These cells showed partial loss of endogenous EWS-Fli-1 proteins and suppression of the cell growth. To elucidate the molecular mechanisms underlying the growth inhibition, we examined the changes of signal transducing proteins by immunoblot analysis in Ewing's sarcoma cells stably expressing antisense EWS-Fli-1 transcripts. Western blotting of the cell proteins revealed that expressions of phospholipase Cbeta2 and beta3 (PLCbeta2, PLCbeta3), and also protein kinase C alpha and beta (PKCalpha, beta) were significantly reduced by transfecting with antisense EWS-Fli-1. The inositol phosphates production by bradykinin (BK), but not platelet-derived growth factor (PDGF), was suppressed in these cells. These results suggest that the PLCbeta2 and PLCbeta3 may play a role in tumour proliferation in Ewing's sarcoma cells. SN - 0007-0920 UR - https://www.unboundmedicine.com/medline/citation/10638960/Preferential_down_regulation_of_phospholipase_C_beta_in_Ewing's_sarcoma_cells_transfected_with_antisense_EWS_Fli_1_ L2 - https://doi.org/10.1054/bjoc.1998.0870 DB - PRIME DP - Unbound Medicine ER -