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Acridine orange stained blood wet mounts for fluorescent detection of malaria.
Indian J Pathol Microbiol. 1999 Apr; 42(2):125-8.IJ

Abstract

The age old Romanowsky stained thick blood smear examination for malarial parasites may fail to reveal the low parasitaemia. The commercial 'QBC' like acridine orange stained capillary tube preparation has a limitation of precise species identification and the detection of extra-erythrocytic parasites. Hence, the present study was aimed to improve malarial parasite detection by using acridine orange to stain large blood drops in the form of wet coverglass mounts. The acridine orange stained blood wet mounts over 2420 suspected malaria cases from Indore city were examined under fluorescent microscope and the results compared with the Leishman's stained thick blood smears in a blind study. The positivity of malarial parasites reported by the modified acridine orange staining was 248 against 109 by Leishman's stained thick blood smears. The modified acridine orange stained method is simple, instant and more efficient, requires less scanning time and skill, allows scanning of larger blood volume (75 ul) at lower magnification and the morphological details at higher magnification helps to make the precise species identification.

Authors+Show Affiliations

Hemvani N
Department Pathology, Choithram Hospital, Indore, India.
Chitnis DS
No affiliation info available
Dixit DS
No affiliation info available
Asolkar MV
No affiliation info available

MeSH

Acridine OrangeAnimalsFluorescent DyesHumansMalariaMalaria, FalciparumMalaria, VivaxMicroscopy, FluorescencePlasmodium falciparumPlasmodium vivaxStaining and Labeling

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

10639770

Citation

Hemvani, N, et al. "Acridine Orange Stained Blood Wet Mounts for Fluorescent Detection of Malaria." Indian Journal of Pathology & Microbiology, vol. 42, no. 2, 1999, pp. 125-8.
Hemvani N, Chitnis DS, Dixit DS, et al. Acridine orange stained blood wet mounts for fluorescent detection of malaria. Indian J Pathol Microbiol. 1999;42(2):125-8.
Hemvani, N., Chitnis, D. S., Dixit, D. S., & Asolkar, M. V. (1999). Acridine orange stained blood wet mounts for fluorescent detection of malaria. Indian Journal of Pathology & Microbiology, 42(2), 125-8.
Hemvani N, et al. Acridine Orange Stained Blood Wet Mounts for Fluorescent Detection of Malaria. Indian J Pathol Microbiol. 1999;42(2):125-8. PubMed PMID: 10639770.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acridine orange stained blood wet mounts for fluorescent detection of malaria. AU - Hemvani,N, AU - Chitnis,D S, AU - Dixit,D S, AU - Asolkar,M V, PY - 2000/1/20/pubmed PY - 2000/2/19/medline PY - 2000/1/20/entrez SP - 125 EP - 8 JF - Indian journal of pathology & microbiology JO - Indian J Pathol Microbiol VL - 42 IS - 2 N2 - The age old Romanowsky stained thick blood smear examination for malarial parasites may fail to reveal the low parasitaemia. The commercial 'QBC' like acridine orange stained capillary tube preparation has a limitation of precise species identification and the detection of extra-erythrocytic parasites. Hence, the present study was aimed to improve malarial parasite detection by using acridine orange to stain large blood drops in the form of wet coverglass mounts. The acridine orange stained blood wet mounts over 2420 suspected malaria cases from Indore city were examined under fluorescent microscope and the results compared with the Leishman's stained thick blood smears in a blind study. The positivity of malarial parasites reported by the modified acridine orange staining was 248 against 109 by Leishman's stained thick blood smears. The modified acridine orange stained method is simple, instant and more efficient, requires less scanning time and skill, allows scanning of larger blood volume (75 ul) at lower magnification and the morphological details at higher magnification helps to make the precise species identification. SN - 0377-4929 UR - https://www.unboundmedicine.com/medline/citation/10639770/Acridine_orange_stained_blood_wet_mounts_for_fluorescent_detection_of_malaria_ L2 - http://www.diseaseinfosearch.org/result/4415 DB - PRIME DP - Unbound Medicine ER -