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Stress response and expression patterns in wine fermentations of yeast genes induced at the diauxic shift.
Yeast. 2000 Jan 30; 16(2):139-48.Y

Abstract

During wine fermentation yeasts quickly reach a stationary phase, where cells are metabolically active by consuming sugars present in grape must. It is, consequently, of great interest at this stage to identify suitable gene promoters that may be used to induce the expression of genes with enological applications. With this aim, we have studied a group of genes showing an induction peak at the diauxic shift, and possessing stress response elements (STRE) at their promoters. We have determined their induction levels under individualized stress conditions, such as carbon source starvation or high salt concentrations. In all the cases studied, the activation and/or basal transcription are dependent on the transcriptional factors Msn2p and Msn4p. We have analysed the expression patterns and mRNA levels during wine fermentation, and have found that they are all activated at the stationary phase. Finally, we have identified SPI1, a new highly expressed yeast gene which is specifically induced at the stationary phase of both microvinification and laboratory growth conditions.

Authors+Show Affiliations

Departamento de Bioquímica y Biología Molecular, Universitat de València, Spain.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10641036

Citation

Puig, S, and J E. Pérez-Ortín. "Stress Response and Expression Patterns in Wine Fermentations of Yeast Genes Induced at the Diauxic Shift." Yeast (Chichester, England), vol. 16, no. 2, 2000, pp. 139-48.
Puig S, Pérez-Ortín JE. Stress response and expression patterns in wine fermentations of yeast genes induced at the diauxic shift. Yeast. 2000;16(2):139-48.
Puig, S., & Pérez-Ortín, J. E. (2000). Stress response and expression patterns in wine fermentations of yeast genes induced at the diauxic shift. Yeast (Chichester, England), 16(2), 139-48.
Puig S, Pérez-Ortín JE. Stress Response and Expression Patterns in Wine Fermentations of Yeast Genes Induced at the Diauxic Shift. Yeast. 2000 Jan 30;16(2):139-48. PubMed PMID: 10641036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stress response and expression patterns in wine fermentations of yeast genes induced at the diauxic shift. AU - Puig,S, AU - Pérez-Ortín,J E, PY - 2000/1/21/pubmed PY - 2000/3/18/medline PY - 2000/1/21/entrez SP - 139 EP - 48 JF - Yeast (Chichester, England) JO - Yeast VL - 16 IS - 2 N2 - During wine fermentation yeasts quickly reach a stationary phase, where cells are metabolically active by consuming sugars present in grape must. It is, consequently, of great interest at this stage to identify suitable gene promoters that may be used to induce the expression of genes with enological applications. With this aim, we have studied a group of genes showing an induction peak at the diauxic shift, and possessing stress response elements (STRE) at their promoters. We have determined their induction levels under individualized stress conditions, such as carbon source starvation or high salt concentrations. In all the cases studied, the activation and/or basal transcription are dependent on the transcriptional factors Msn2p and Msn4p. We have analysed the expression patterns and mRNA levels during wine fermentation, and have found that they are all activated at the stationary phase. Finally, we have identified SPI1, a new highly expressed yeast gene which is specifically induced at the stationary phase of both microvinification and laboratory growth conditions. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/10641036/Stress_response_and_expression_patterns_in_wine_fermentations_of_yeast_genes_induced_at_the_diauxic_shift_ L2 - https://doi.org/10.1002/(SICI)1097-0061(20000130)16:2<139::AID-YEA512>3.0.CO;2-J DB - PRIME DP - Unbound Medicine ER -