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LMP2 polymorphism is associated with extraspinal disease in HLA-B27 negative Caucasian and Mexican Mestizo patients with ankylosing spondylitis.
J Rheumatol. 2000 Jan; 27(1):183-9.JR

Abstract

OBJECTIVE

To determine the effects of HLA Class II genes, particularly LMP2 and previously implicated Class I genes, on susceptibility and disease expression in HLA-B27 negative ankylosing spondylitis (AS).

METHODS

Patients included 41 HLA-B27 negative Caucasians from a total AS population of 546 and 17 HLA-B27 negative Mexican Mestizo. Controls included 4352 random HLA-B27 negative Caucasians. LMP2 genotype assignments were made on all patients and 282 random Caucasian controls by polymerase chain reaction-restriction fragment length polymorphism with the Cfo I restriction enzyme while HLA typing was performed on patients and controls using microcytotoxicity assays for Class I, and sequence specific probe-PCR for HLA-B60, B39, B38, and DR.

RESULTS

The LMP2BB genotype was significantly decreased in Caucasian AS patients without extraspinal (ES) disease (25%) compared to AS patients with ES (64.7%) (p = 0.01) and random Caucasian controls (53.9%) (p = 0.007), even when those with colitis and psoriasis were excluded from analysis (ES+ 55.6% versus ES- 22.2%). This finding remained significant after stratification by HLA-DR. Similar trends were noted in the Mexican population. A potential role for HLA-DR8 and DR2 in susceptibility to disease was observed in Caucasian patients, although this observation requires confirmation. We could not confirm reported associations with HLA-B60 or B39. Peripheral arthritis was significantly more commonly observed in those who had had acute anterior uveitis (AAU) (75%) than in those who had not developed AAU (27.3%) (p = 0.04).

CONCLUSION

HLA Class II encoded genes may have effects on disease susceptibility and/or phenotype in HLA-B27 negative individuals similar to those noted in HLA-B27 positive AS. Eccentric and axial phenotypes of disease may be immunogenetically determined.

Authors+Show Affiliations

Department of Medicine, University of Alberta, Edmonton, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10648037

Citation

Maksymowych, W P., et al. "LMP2 Polymorphism Is Associated With Extraspinal Disease in HLA-B27 Negative Caucasian and Mexican Mestizo Patients With Ankylosing Spondylitis." The Journal of Rheumatology, vol. 27, no. 1, 2000, pp. 183-9.
Maksymowych WP, Tao S, Vaile J, et al. LMP2 polymorphism is associated with extraspinal disease in HLA-B27 negative Caucasian and Mexican Mestizo patients with ankylosing spondylitis. J Rheumatol. 2000;27(1):183-9.
Maksymowych, W. P., Tao, S., Vaile, J., Suarez-Almazor, M., Ramos-Remus, C., & Russell, A. S. (2000). LMP2 polymorphism is associated with extraspinal disease in HLA-B27 negative Caucasian and Mexican Mestizo patients with ankylosing spondylitis. The Journal of Rheumatology, 27(1), 183-9.
Maksymowych WP, et al. LMP2 Polymorphism Is Associated With Extraspinal Disease in HLA-B27 Negative Caucasian and Mexican Mestizo Patients With Ankylosing Spondylitis. J Rheumatol. 2000;27(1):183-9. PubMed PMID: 10648037.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LMP2 polymorphism is associated with extraspinal disease in HLA-B27 negative Caucasian and Mexican Mestizo patients with ankylosing spondylitis. AU - Maksymowych,W P, AU - Tao,S, AU - Vaile,J, AU - Suarez-Almazor,M, AU - Ramos-Remus,C, AU - Russell,A S, PY - 2000/1/27/pubmed PY - 2000/2/26/medline PY - 2000/1/27/entrez SP - 183 EP - 9 JF - The Journal of rheumatology JO - J Rheumatol VL - 27 IS - 1 N2 - OBJECTIVE: To determine the effects of HLA Class II genes, particularly LMP2 and previously implicated Class I genes, on susceptibility and disease expression in HLA-B27 negative ankylosing spondylitis (AS). METHODS: Patients included 41 HLA-B27 negative Caucasians from a total AS population of 546 and 17 HLA-B27 negative Mexican Mestizo. Controls included 4352 random HLA-B27 negative Caucasians. LMP2 genotype assignments were made on all patients and 282 random Caucasian controls by polymerase chain reaction-restriction fragment length polymorphism with the Cfo I restriction enzyme while HLA typing was performed on patients and controls using microcytotoxicity assays for Class I, and sequence specific probe-PCR for HLA-B60, B39, B38, and DR. RESULTS: The LMP2BB genotype was significantly decreased in Caucasian AS patients without extraspinal (ES) disease (25%) compared to AS patients with ES (64.7%) (p = 0.01) and random Caucasian controls (53.9%) (p = 0.007), even when those with colitis and psoriasis were excluded from analysis (ES+ 55.6% versus ES- 22.2%). This finding remained significant after stratification by HLA-DR. Similar trends were noted in the Mexican population. A potential role for HLA-DR8 and DR2 in susceptibility to disease was observed in Caucasian patients, although this observation requires confirmation. We could not confirm reported associations with HLA-B60 or B39. Peripheral arthritis was significantly more commonly observed in those who had had acute anterior uveitis (AAU) (75%) than in those who had not developed AAU (27.3%) (p = 0.04). CONCLUSION: HLA Class II encoded genes may have effects on disease susceptibility and/or phenotype in HLA-B27 negative individuals similar to those noted in HLA-B27 positive AS. Eccentric and axial phenotypes of disease may be immunogenetically determined. SN - 0315-162X UR - https://www.unboundmedicine.com/medline/citation/10648037/LMP2_polymorphism_is_associated_with_extraspinal_disease_in_HLA_B27_negative_Caucasian_and_Mexican_Mestizo_patients_with_ankylosing_spondylitis_ L2 - http://www.diseaseinfosearch.org/result/482 DB - PRIME DP - Unbound Medicine ER -