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RAD51 is required for the repair of plasmid double-stranded DNA gaps from either plasmid or chromosomal templates.
Mol Cell Biol. 2000 Feb; 20(4):1194-205.MC

Abstract

DNA double-strand breaks may be induced by endonucleases, ionizing radiation, chemical agents, and mechanical forces or by replication of single-stranded nicked chromosomes. Repair of double-strand breaks can occur by homologous recombination or by nonhomologous end joining. A system was developed to measure the efficiency of plasmid gap repair by homologous recombination using either chromosomal or plasmid templates. Gap repair was biased toward gene conversion events unassociated with crossing over using either donor sequence. The dependence of recombinational gap repair on genes belonging to the RAD52 epistasis group was tested in this system. RAD51, RAD52, RAD57, and RAD59 were required for efficient gap repair using either chromosomal or plasmid donors. No homologous recombination products were recovered from rad52 mutants, whereas a low level of repair occurred in the absence of RAD51, RAD57, or RAD59. These results suggest a minor pathway of strand invasion that is dependent on RAD52 but not on RAD51. The residual repair events in rad51 mutants were more frequently associated with crossing over than was observed in the wild-type strain, suggesting that the mechanisms for RAD51-dependent and RAD51-independent events are different. Plasmid gap repair was reduced synergistically in rad51 rad59 double mutants, indicating an important role for RAD59 in RAD51-independent repair.

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Authors+Show Affiliations

Bärtsch S
Department of Microbiology and Institute of Cancer Research, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
Kang LE
No affiliation info available
Symington LS
No affiliation info available

MeSH

Amino Acid SequenceBase SequenceCell Cycle ProteinsCheckpoint Kinase 2Chromosomes, FungalCrossing Over, GeneticDNA PrimersDNA RepairDNA, FungalDNA-Binding ProteinsEpistasis, GeneticFungal ProteinsGenes, FungalModels, BiologicalMutationPlasmidsProtein KinasesProtein-Serine-Threonine KinasesRad51 RecombinaseRad52 DNA Repair and Recombination ProteinRecombination, GeneticSaccharomyces cerevisiaeSaccharomyces cerevisiae Proteins

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10648605

Citation

Bärtsch, S, et al. "RAD51 Is Required for the Repair of Plasmid Double-stranded DNA Gaps From Either Plasmid or Chromosomal Templates." Molecular and Cellular Biology, vol. 20, no. 4, 2000, pp. 1194-205.
Bärtsch S, Kang LE, Symington LS. RAD51 is required for the repair of plasmid double-stranded DNA gaps from either plasmid or chromosomal templates. Mol Cell Biol. 2000;20(4):1194-205.
Bärtsch, S., Kang, L. E., & Symington, L. S. (2000). RAD51 is required for the repair of plasmid double-stranded DNA gaps from either plasmid or chromosomal templates. Molecular and Cellular Biology, 20(4), 1194-205.
Bärtsch S, Kang LE, Symington LS. RAD51 Is Required for the Repair of Plasmid Double-stranded DNA Gaps From Either Plasmid or Chromosomal Templates. Mol Cell Biol. 2000;20(4):1194-205. PubMed PMID: 10648605.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RAD51 is required for the repair of plasmid double-stranded DNA gaps from either plasmid or chromosomal templates. AU - Bärtsch,S, AU - Kang,L E, AU - Symington,L S, PY - 2000/1/29/pubmed PY - 2000/2/19/medline PY - 2000/1/29/entrez SP - 1194 EP - 205 JF - Molecular and cellular biology JO - Mol Cell Biol VL - 20 IS - 4 N2 - DNA double-strand breaks may be induced by endonucleases, ionizing radiation, chemical agents, and mechanical forces or by replication of single-stranded nicked chromosomes. Repair of double-strand breaks can occur by homologous recombination or by nonhomologous end joining. A system was developed to measure the efficiency of plasmid gap repair by homologous recombination using either chromosomal or plasmid templates. Gap repair was biased toward gene conversion events unassociated with crossing over using either donor sequence. The dependence of recombinational gap repair on genes belonging to the RAD52 epistasis group was tested in this system. RAD51, RAD52, RAD57, and RAD59 were required for efficient gap repair using either chromosomal or plasmid donors. No homologous recombination products were recovered from rad52 mutants, whereas a low level of repair occurred in the absence of RAD51, RAD57, or RAD59. These results suggest a minor pathway of strand invasion that is dependent on RAD52 but not on RAD51. The residual repair events in rad51 mutants were more frequently associated with crossing over than was observed in the wild-type strain, suggesting that the mechanisms for RAD51-dependent and RAD51-independent events are different. Plasmid gap repair was reduced synergistically in rad51 rad59 double mutants, indicating an important role for RAD59 in RAD51-independent repair. SN - 0270-7306 UR - https://www.unboundmedicine.com/medline/citation/10648605/RAD51_is_required_for_the_repair_of_plasmid_double_stranded_DNA_gaps_from_either_plasmid_or_chromosomal_templates_ L2 - https://journals.asm.org/doi/10.1128/MCB.20.4.1194-1205.2000?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -