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Identification and purification of the 69-kDa intracellular protease involved in the proteolytic processing of the crystal delta-endotoxin of Bacillus thuringiensis subsp. tenebrionis.
FEMS Microbiol Lett. 2000 Feb 01; 183(1):63-6.FM

Abstract

The dynamics of appearance of intracellular proteases in relation to the synthesis of crystal delta-endotoxin was studied to identify the native intracellular protease(s) involved in the proteolytic processing of the 73-kDa protoxin of Bacillus thuringiensis subsp. tenebrionis. In vitro proteolytic activation of the 73-kDa protoxin indicated the possible role of 69-kDa protease in the proteolytic processing of 73-kDa protoxin. The purified 69-kDa protease was able to cause the proteolytic activation of the 73-kDa protoxin to 68-kDa toxin and this conversion was inhibited by ethylenediamine tetraacetic acid and 1,10-phenanthroline.

Links

Publisher Full Text
academic.oup.com
onlinelibrary.wiley.com

Authors+Show Affiliations

Reddy ST
Department of Biochemistry, Osmania University, Hyderabad, India.
Kumar NS
No affiliation info available
Venkateswerlu G
No affiliation info available

MeSH

Bacillus thuringiensisBacillus thuringiensis ToxinsBacterial ProteinsBacterial ToxinsElectrophoresis, Polyacrylamide GelEndopeptidasesEndotoxinsHemolysin ProteinsHumansImmunoblottingMetalloendopeptidasesProtease Inhibitors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10650203

Citation

Reddy, S T., et al. "Identification and Purification of the 69-kDa Intracellular Protease Involved in the Proteolytic Processing of the Crystal Delta-endotoxin of Bacillus Thuringiensis Subsp. Tenebrionis." FEMS Microbiology Letters, vol. 183, no. 1, 2000, pp. 63-6.
Reddy ST, Kumar NS, Venkateswerlu G. Identification and purification of the 69-kDa intracellular protease involved in the proteolytic processing of the crystal delta-endotoxin of Bacillus thuringiensis subsp. tenebrionis. FEMS Microbiol Lett. 2000;183(1):63-6.
Reddy, S. T., Kumar, N. S., & Venkateswerlu, G. (2000). Identification and purification of the 69-kDa intracellular protease involved in the proteolytic processing of the crystal delta-endotoxin of Bacillus thuringiensis subsp. tenebrionis. FEMS Microbiology Letters, 183(1), 63-6.
Reddy ST, Kumar NS, Venkateswerlu G. Identification and Purification of the 69-kDa Intracellular Protease Involved in the Proteolytic Processing of the Crystal Delta-endotoxin of Bacillus Thuringiensis Subsp. Tenebrionis. FEMS Microbiol Lett. 2000 Feb 1;183(1):63-6. PubMed PMID: 10650203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and purification of the 69-kDa intracellular protease involved in the proteolytic processing of the crystal delta-endotoxin of Bacillus thuringiensis subsp. tenebrionis. AU - Reddy,S T, AU - Kumar,N S, AU - Venkateswerlu,G, PY - 2000/1/29/pubmed PY - 2000/3/11/medline PY - 2000/1/29/entrez SP - 63 EP - 6 JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 183 IS - 1 N2 - The dynamics of appearance of intracellular proteases in relation to the synthesis of crystal delta-endotoxin was studied to identify the native intracellular protease(s) involved in the proteolytic processing of the 73-kDa protoxin of Bacillus thuringiensis subsp. tenebrionis. In vitro proteolytic activation of the 73-kDa protoxin indicated the possible role of 69-kDa protease in the proteolytic processing of 73-kDa protoxin. The purified 69-kDa protease was able to cause the proteolytic activation of the 73-kDa protoxin to 68-kDa toxin and this conversion was inhibited by ethylenediamine tetraacetic acid and 1,10-phenanthroline. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/10650203/Identification_and_purification_of_the_69_kDa_intracellular_protease_involved_in_the_proteolytic_processing_of_the_crystal_delta_endotoxin_of_Bacillus_thuringiensis_subsp__tenebrionis_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.2000.tb08934.x DB - PRIME DP - Unbound Medicine ER -