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Sexuality and genetic identity in the Agaricus section Arvenses.

Abstract

Twelve wild collections and one commercial strain were used to characterize breeding systems and to develop molecular identities in the Arvenses section of the genus Agaricus, which includes the "horse mushroom" A. arvensis. Two morphotypes were identified based on macro- and micromorphological features. However, not all collections could be delimited by conventional taxonomic characters. Sequencing of the small subunit intergenic spacer (ITS) region (368 to 370 bp) of the rRNA genes clearly resolved the 13 collections into two clusters consistent with the identified morphotypes. Single-spore progenies and mating type testers were established and used to test intra- and interstock compatibility. The two compatibility groups identified were consistent with ITS clusters. Compatibility group I stocks readily interbred within the constraints of a unifactorial heterothallic system with a multiallelic mating type factor. Compatibility group II had a more restricted breeding pattern, and interactions were difficult to predict on the basis of mating type. Morphological data, ITS sequences, and the ability to interbreed suggest that these collections are part of a complex of interrelated species. Single-spore, homokaryotic isolates from both compatibility groups were able to fruit in compost culture, and two of the collections may represent natural homokaryotic fruiting. We conclude that species from the section Arvenses have versatile unifactorial heterothallic life cycles that permit both interbreeding and homokaryotic fruiting.

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  • Authors+Show Affiliations

    ,

    Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF, United Kingdom.

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    Source

    MeSH

    Agaricus
    DNA, Fungal
    DNA, Ribosomal
    Genes, rRNA
    Molecular Sequence Data
    RNA, Ribosomal
    Random Amplified Polymorphic DNA Technique
    Reproduction
    Sequence Analysis, DNA
    Spores, Fungal

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    10653743

    Citation

    * When formatting your citation, note that all book, journal, and database titles should be italicized* Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Sexuality and genetic identity in the Agaricus section Arvenses. AU - Calvo-Bado,L, AU - Noble,R, AU - Challen,M, AU - Dobrovin-Pennington,A, AU - Elliott,T, PY - 2000/2/2/pubmed PY - 2000/3/18/medline PY - 2000/2/2/entrez SP - 728 EP - 34 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 66 IS - 2 N2 - Twelve wild collections and one commercial strain were used to characterize breeding systems and to develop molecular identities in the Arvenses section of the genus Agaricus, which includes the "horse mushroom" A. arvensis. Two morphotypes were identified based on macro- and micromorphological features. However, not all collections could be delimited by conventional taxonomic characters. Sequencing of the small subunit intergenic spacer (ITS) region (368 to 370 bp) of the rRNA genes clearly resolved the 13 collections into two clusters consistent with the identified morphotypes. Single-spore progenies and mating type testers were established and used to test intra- and interstock compatibility. The two compatibility groups identified were consistent with ITS clusters. Compatibility group I stocks readily interbred within the constraints of a unifactorial heterothallic system with a multiallelic mating type factor. Compatibility group II had a more restricted breeding pattern, and interactions were difficult to predict on the basis of mating type. Morphological data, ITS sequences, and the ability to interbreed suggest that these collections are part of a complex of interrelated species. Single-spore, homokaryotic isolates from both compatibility groups were able to fruit in compost culture, and two of the collections may represent natural homokaryotic fruiting. We conclude that species from the section Arvenses have versatile unifactorial heterothallic life cycles that permit both interbreeding and homokaryotic fruiting. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/10653743/Sexuality_and_genetic_identity_in_the_Agaricus_section_Arvenses_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=10653743 DB - PRIME DP - Unbound Medicine ER -