Tags

Type your tag names separated by a space and hit enter

Sexuality and genetic identity in the Agaricus section Arvenses.
Appl Environ Microbiol 2000; 66(2):728-34AE

Abstract

Twelve wild collections and one commercial strain were used to characterize breeding systems and to develop molecular identities in the Arvenses section of the genus Agaricus, which includes the "horse mushroom" A. arvensis. Two morphotypes were identified based on macro- and micromorphological features. However, not all collections could be delimited by conventional taxonomic characters. Sequencing of the small subunit intergenic spacer (ITS) region (368 to 370 bp) of the rRNA genes clearly resolved the 13 collections into two clusters consistent with the identified morphotypes. Single-spore progenies and mating type testers were established and used to test intra- and interstock compatibility. The two compatibility groups identified were consistent with ITS clusters. Compatibility group I stocks readily interbred within the constraints of a unifactorial heterothallic system with a multiallelic mating type factor. Compatibility group II had a more restricted breeding pattern, and interactions were difficult to predict on the basis of mating type. Morphological data, ITS sequences, and the ability to interbreed suggest that these collections are part of a complex of interrelated species. Single-spore, homokaryotic isolates from both compatibility groups were able to fruit in compost culture, and two of the collections may represent natural homokaryotic fruiting. We conclude that species from the section Arvenses have versatile unifactorial heterothallic life cycles that permit both interbreeding and homokaryotic fruiting.

Authors+Show Affiliations

Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10653743

Citation

Calvo-Bado, L, et al. "Sexuality and Genetic Identity in the Agaricus Section Arvenses." Applied and Environmental Microbiology, vol. 66, no. 2, 2000, pp. 728-34.
Calvo-Bado L, Noble R, Challen M, et al. Sexuality and genetic identity in the Agaricus section Arvenses. Appl Environ Microbiol. 2000;66(2):728-34.
Calvo-Bado, L., Noble, R., Challen, M., Dobrovin-Pennington, A., & Elliott, T. (2000). Sexuality and genetic identity in the Agaricus section Arvenses. Applied and Environmental Microbiology, 66(2), pp. 728-34.
Calvo-Bado L, et al. Sexuality and Genetic Identity in the Agaricus Section Arvenses. Appl Environ Microbiol. 2000;66(2):728-34. PubMed PMID: 10653743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sexuality and genetic identity in the Agaricus section Arvenses. AU - Calvo-Bado,L, AU - Noble,R, AU - Challen,M, AU - Dobrovin-Pennington,A, AU - Elliott,T, PY - 2000/2/2/pubmed PY - 2000/3/18/medline PY - 2000/2/2/entrez SP - 728 EP - 34 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 66 IS - 2 N2 - Twelve wild collections and one commercial strain were used to characterize breeding systems and to develop molecular identities in the Arvenses section of the genus Agaricus, which includes the "horse mushroom" A. arvensis. Two morphotypes were identified based on macro- and micromorphological features. However, not all collections could be delimited by conventional taxonomic characters. Sequencing of the small subunit intergenic spacer (ITS) region (368 to 370 bp) of the rRNA genes clearly resolved the 13 collections into two clusters consistent with the identified morphotypes. Single-spore progenies and mating type testers were established and used to test intra- and interstock compatibility. The two compatibility groups identified were consistent with ITS clusters. Compatibility group I stocks readily interbred within the constraints of a unifactorial heterothallic system with a multiallelic mating type factor. Compatibility group II had a more restricted breeding pattern, and interactions were difficult to predict on the basis of mating type. Morphological data, ITS sequences, and the ability to interbreed suggest that these collections are part of a complex of interrelated species. Single-spore, homokaryotic isolates from both compatibility groups were able to fruit in compost culture, and two of the collections may represent natural homokaryotic fruiting. We conclude that species from the section Arvenses have versatile unifactorial heterothallic life cycles that permit both interbreeding and homokaryotic fruiting. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/10653743/Sexuality_and_genetic_identity_in_the_Agaricus_section_Arvenses_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=10653743 DB - PRIME DP - Unbound Medicine ER -