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Quantitation of caffeine by capillary zone electrophoresis with end-column amperometric detection at a carbon microdisk array electrode.
J Chromatogr Sci. 2000 Jan; 38(1):11-5.JC

Abstract

Capillary zone electrophoresis is employed for the determination of caffeine using end-column amperometric detection with a carbon fiber microdisk array electrode at a constant potential of 1.45 V versus a saturated calomel electrode. The optimum conditions of separation and detection are 0.1 52mM NaH2PO4-0.648mM Na2HPO4 for the buffer solution, 20 kV for the separation voltage, 5 kV for the injection voltage, and 10s for the injection time. The limit of detection is 2.9 x 10(-4)mM or 1.2 fmol (signal-to-noise ratio = 2). The relative standard deviation is 0.68% for the migration time and 2.3% for the electrophoretic peak current. The method is applied to determining caffeine in human serum and a cola drink.

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Authors+Show Affiliations

Jin W
Laboratory of Analytical Science, School of Chemistry, Shandong University, China.
Yu D
No affiliation info available
Dong Q
No affiliation info available
Ye X
No affiliation info available

MeSH

BeveragesCaffeineCarbonElectrodesElectrophoresis, CapillaryHumansHydrogen-Ion ConcentrationReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10654786

Citation

Jin, W, et al. "Quantitation of Caffeine By Capillary Zone Electrophoresis With End-column Amperometric Detection at a Carbon Microdisk Array Electrode." Journal of Chromatographic Science, vol. 38, no. 1, 2000, pp. 11-5.
Jin W, Yu D, Dong Q, et al. Quantitation of caffeine by capillary zone electrophoresis with end-column amperometric detection at a carbon microdisk array electrode. J Chromatogr Sci. 2000;38(1):11-5.
Jin, W., Yu, D., Dong, Q., & Ye, X. (2000). Quantitation of caffeine by capillary zone electrophoresis with end-column amperometric detection at a carbon microdisk array electrode. Journal of Chromatographic Science, 38(1), 11-5.
Jin W, et al. Quantitation of Caffeine By Capillary Zone Electrophoresis With End-column Amperometric Detection at a Carbon Microdisk Array Electrode. J Chromatogr Sci. 2000;38(1):11-5. PubMed PMID: 10654786.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitation of caffeine by capillary zone electrophoresis with end-column amperometric detection at a carbon microdisk array electrode. AU - Jin,W, AU - Yu,D, AU - Dong,Q, AU - Ye,X, PY - 2000/2/2/pubmed PY - 2000/2/19/medline PY - 2000/2/2/entrez SP - 11 EP - 5 JF - Journal of chromatographic science JO - J Chromatogr Sci VL - 38 IS - 1 N2 - Capillary zone electrophoresis is employed for the determination of caffeine using end-column amperometric detection with a carbon fiber microdisk array electrode at a constant potential of 1.45 V versus a saturated calomel electrode. The optimum conditions of separation and detection are 0.1 52mM NaH2PO4-0.648mM Na2HPO4 for the buffer solution, 20 kV for the separation voltage, 5 kV for the injection voltage, and 10s for the injection time. The limit of detection is 2.9 x 10(-4)mM or 1.2 fmol (signal-to-noise ratio = 2). The relative standard deviation is 0.68% for the migration time and 2.3% for the electrophoretic peak current. The method is applied to determining caffeine in human serum and a cola drink. SN - 0021-9665 UR - https://www.unboundmedicine.com/medline/citation/10654786/Quantitation_of_caffeine_by_capillary_zone_electrophoresis_with_end_column_amperometric_detection_at_a_carbon_microdisk_array_electrode_ L2 - https://academic.oup.com/chromsci/article-lookup/doi/10.1093/chromsci/38.1.11 DB - PRIME DP - Unbound Medicine ER -