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Effects of conjugated linoleic acid (CLA) isomers on lipid levels and peroxisome proliferation in the hamster.
Br J Nutr. 1999 Oct; 82(4):309-17.BJ

Abstract

Effects of the conjugated linoleic acid (CLA) isomers cis-9, trans-11 (c9,t11 CLA) and trans-10, cis-12 (t10,c12 CLA) on lipid metabolism and markers of peroxisome proliferation were investigated in hamsters fed on purified diets containing 30% energy as fat and 0.1 g cholesterol/kg for 8 weeks. Four groups (n 32 each) received diets without CLA (control), with a mixture of equal amounts of c9,t11 and t10,c12 CLA (CLA mix), with c9,t11 CLA, and with t10,c12 CLA. The total amount of CLA isomers was 1.5% energy of 6.6g/ kg diet. CLA was incorporated into glycerides and exchanged for linoleic acid in the diet. Compared with the control, the CLA mix and t10,c12 CLA decreased fasting values of LDL- (21 and 18% respectively) and HDL-cholesterol (8 and 11%), increased VLDL-triacylglycerol (80 and 61%, and decreased epididymal fat pad weights (9 and 16%), whereas c9,t11 CLA had no significant effects. All CLA preparations increased liver weight, but not liver lipids. However, the increase in liver weight was much less in the c9,t11 CLA group (8%) than in the other two groups (25%) and might have been caused by the small amount of t10,c12 CLA present in the c9,t11 CLA preparation. Liver histology revealed that increased weight was due to hypertrophy. Markers of peroxisome proliferation, such as cyanide-insensitive palmitoyl CoA oxidase (EC 1.3.3.6) and carnitine acetyl transferase (EC 2.3.1.7) activities, were not increased by CLA. Both c9,t11 CLA and t10,c12 CLA were incorporated into phospholipids and triacylglycerols, but t10,c12 CLA only about half as much as c9,t11 CLA. In addition, linoleic acid and linolenic acid concentrations were lower in lipids of the t10,c12 CLA group compared with the c9,t11 CLA group. These data suggest that t10,c12 CLA stimulated the oxidation of all C18 polyunsaturated fatty acids. The results indicate that the t10,c12 CLA isomer, and not the so-called natural CLA isomer (c9,t11), is the active isomer affecting lipid levels in hamsters.

Authors+Show Affiliations

Unilever Research Vlaardingen, The Netherlands. emile-de.deckere@unilever.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10655980

Citation

de Deckere, E A., et al. "Effects of Conjugated Linoleic Acid (CLA) Isomers On Lipid Levels and Peroxisome Proliferation in the Hamster." The British Journal of Nutrition, vol. 82, no. 4, 1999, pp. 309-17.
de Deckere EA, van Amelsvoort JM, McNeill GP, et al. Effects of conjugated linoleic acid (CLA) isomers on lipid levels and peroxisome proliferation in the hamster. Br J Nutr. 1999;82(4):309-17.
de Deckere, E. A., van Amelsvoort, J. M., McNeill, G. P., & Jones, P. (1999). Effects of conjugated linoleic acid (CLA) isomers on lipid levels and peroxisome proliferation in the hamster. The British Journal of Nutrition, 82(4), 309-17.
de Deckere EA, et al. Effects of Conjugated Linoleic Acid (CLA) Isomers On Lipid Levels and Peroxisome Proliferation in the Hamster. Br J Nutr. 1999;82(4):309-17. PubMed PMID: 10655980.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of conjugated linoleic acid (CLA) isomers on lipid levels and peroxisome proliferation in the hamster. AU - de Deckere,E A, AU - van Amelsvoort,J M, AU - McNeill,G P, AU - Jones,P, PY - 2000/2/3/pubmed PY - 2000/2/19/medline PY - 2000/2/3/entrez SP - 309 EP - 17 JF - The British journal of nutrition JO - Br J Nutr VL - 82 IS - 4 N2 - Effects of the conjugated linoleic acid (CLA) isomers cis-9, trans-11 (c9,t11 CLA) and trans-10, cis-12 (t10,c12 CLA) on lipid metabolism and markers of peroxisome proliferation were investigated in hamsters fed on purified diets containing 30% energy as fat and 0.1 g cholesterol/kg for 8 weeks. Four groups (n 32 each) received diets without CLA (control), with a mixture of equal amounts of c9,t11 and t10,c12 CLA (CLA mix), with c9,t11 CLA, and with t10,c12 CLA. The total amount of CLA isomers was 1.5% energy of 6.6g/ kg diet. CLA was incorporated into glycerides and exchanged for linoleic acid in the diet. Compared with the control, the CLA mix and t10,c12 CLA decreased fasting values of LDL- (21 and 18% respectively) and HDL-cholesterol (8 and 11%), increased VLDL-triacylglycerol (80 and 61%, and decreased epididymal fat pad weights (9 and 16%), whereas c9,t11 CLA had no significant effects. All CLA preparations increased liver weight, but not liver lipids. However, the increase in liver weight was much less in the c9,t11 CLA group (8%) than in the other two groups (25%) and might have been caused by the small amount of t10,c12 CLA present in the c9,t11 CLA preparation. Liver histology revealed that increased weight was due to hypertrophy. Markers of peroxisome proliferation, such as cyanide-insensitive palmitoyl CoA oxidase (EC 1.3.3.6) and carnitine acetyl transferase (EC 2.3.1.7) activities, were not increased by CLA. Both c9,t11 CLA and t10,c12 CLA were incorporated into phospholipids and triacylglycerols, but t10,c12 CLA only about half as much as c9,t11 CLA. In addition, linoleic acid and linolenic acid concentrations were lower in lipids of the t10,c12 CLA group compared with the c9,t11 CLA group. These data suggest that t10,c12 CLA stimulated the oxidation of all C18 polyunsaturated fatty acids. The results indicate that the t10,c12 CLA isomer, and not the so-called natural CLA isomer (c9,t11), is the active isomer affecting lipid levels in hamsters. SN - 0007-1145 UR - https://www.unboundmedicine.com/medline/citation/10655980/Effects_of_conjugated_linoleic_acid__CLA__isomers_on_lipid_levels_and_peroxisome_proliferation_in_the_hamster_ L2 - https://www.cambridge.org/core/product/identifier/S0007114599001518/type/journal_article DB - PRIME DP - Unbound Medicine ER -