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Ultrasensitive determination of phencyclidine in body fluids by surface ionization organic mass spectrometry.
Anal Chem. 2000 Jan 15; 72(2):404-5.AC

Abstract

Gas chromatography (GC)/surface ionization organic mass spectrometry (SIOMS) has been found to give much higher sensitivity for measurements of phencyclidine (PCP) than the conventional GC/electron impact (EI)-mass spectrometry (MS). Thus, we have established a detailed procedure for measurements of PCP in body fluids by both mass chromatography and selected-ion monitoring (SIM) of SIOMS using pethidine as an internal standard (IS). Good linearity was found in the range of 0.25-10 ng/mL of whole blood or urine, when measured by mass chromatography, and in the range of 0.025-1.0 ng/mL of whole blood by SIM. The recoveries of PCP and IS spiked to whole blood were 106 +/- 17% at 1 ng/mL and 113 +/- 11% at 5 ng/mL; that of IS was 97.8 +/- 10.4% at 5 ng/mL. The detection limits (signal-to-noise ratio = 3) were estimated to be 0.05 ng/mL of whole blood or urine by mass chromatography and 0.01 ng/mL of whole blood by SIM. The coefficients of intraday and interday variations were not greater than 10.3%. We could detect PCP from rat whole blood 2 h after subcutaneous injection of PCP (1 mg/kg) by mass chromatography. The mean PCP concentration in rat blood was 47.7 +/- 6.2 ng/mL (mean +/- SD, n = 4).

Authors+Show Affiliations

Department of Legal Medicine and Bioethics, Nagoya University Post Graduate School of Medicine, Japan. akishii@med.nagoya-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10658337

Citation

Ishii, A, et al. "Ultrasensitive Determination of Phencyclidine in Body Fluids By Surface Ionization Organic Mass Spectrometry." Analytical Chemistry, vol. 72, no. 2, 2000, pp. 404-5.
Ishii A, Seno H, Watanabe-Suzuki K, et al. Ultrasensitive determination of phencyclidine in body fluids by surface ionization organic mass spectrometry. Anal Chem. 2000;72(2):404-5.
Ishii, A., Seno, H., Watanabe-Suzuki, K., Kumazawa, T., Matsushima, H., Suzuki, O., & Katsumata, Y. (2000). Ultrasensitive determination of phencyclidine in body fluids by surface ionization organic mass spectrometry. Analytical Chemistry, 72(2), 404-5.
Ishii A, et al. Ultrasensitive Determination of Phencyclidine in Body Fluids By Surface Ionization Organic Mass Spectrometry. Anal Chem. 2000 Jan 15;72(2):404-5. PubMed PMID: 10658337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrasensitive determination of phencyclidine in body fluids by surface ionization organic mass spectrometry. AU - Ishii,A, AU - Seno,H, AU - Watanabe-Suzuki,K, AU - Kumazawa,T, AU - Matsushima,H, AU - Suzuki,O, AU - Katsumata,Y, PY - 2000/2/5/pubmed PY - 2000/2/26/medline PY - 2000/2/5/entrez SP - 404 EP - 5 JF - Analytical chemistry JO - Anal Chem VL - 72 IS - 2 N2 - Gas chromatography (GC)/surface ionization organic mass spectrometry (SIOMS) has been found to give much higher sensitivity for measurements of phencyclidine (PCP) than the conventional GC/electron impact (EI)-mass spectrometry (MS). Thus, we have established a detailed procedure for measurements of PCP in body fluids by both mass chromatography and selected-ion monitoring (SIM) of SIOMS using pethidine as an internal standard (IS). Good linearity was found in the range of 0.25-10 ng/mL of whole blood or urine, when measured by mass chromatography, and in the range of 0.025-1.0 ng/mL of whole blood by SIM. The recoveries of PCP and IS spiked to whole blood were 106 +/- 17% at 1 ng/mL and 113 +/- 11% at 5 ng/mL; that of IS was 97.8 +/- 10.4% at 5 ng/mL. The detection limits (signal-to-noise ratio = 3) were estimated to be 0.05 ng/mL of whole blood or urine by mass chromatography and 0.01 ng/mL of whole blood by SIM. The coefficients of intraday and interday variations were not greater than 10.3%. We could detect PCP from rat whole blood 2 h after subcutaneous injection of PCP (1 mg/kg) by mass chromatography. The mean PCP concentration in rat blood was 47.7 +/- 6.2 ng/mL (mean +/- SD, n = 4). SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/10658337/Ultrasensitive_determination_of_phencyclidine_in_body_fluids_by_surface_ionization_organic_mass_spectrometry_ L2 - https://doi.org/10.1021/ac990765t DB - PRIME DP - Unbound Medicine ER -