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Screening postmortem blood and tissues for nine classes [correction of cases] of drugs of abuse using automated microplate immunoassay.
Forensic Sci Int 1999; 106(2):93-102FS

Abstract

A commercially available enzyme-linked immunosorbant assay (ELISA) was evaluated as a screening procedure for the detection of nine classes of abused drugs in postmortem blood and tissue specimens. Specifically, postmortem blood, fluid and/or tissue homogenates were screened for amphetamine (AMP), methamphetamine (MET), barbiturates (BARB), benzodiazepines (BZD), cannabinoids (CNB), cocaine (benzoylecgonine; BE), morphine-specific (MOR), opiates (class; OPI), phencyclidine (PCP) and lysergic acid diethylamide (LSD) by ELISA and by coated tube radioimmunoassay (CTR) (BARB, BE, OPI, PCP, LSD) or double-antibody radioimmunoassay (DAR) (AMP/METH, BZD, CNB). Specimens that screened 'positive' by any method were confirmed and quantitated by gas chromatography/mass spectrometry (GC/MS). The only assay that appeared to perform less optimally than RIA was the MOR assay (five false negatives). However, this assay is very specific for free morphine while the GC/MS confirmation method provided a total morphine value. The OPI assay was more sensitive, producing fewer false negatives, and is recommended for broad class opiate screening. EIA is an adequate alternative to RIA for screening postmortem specimens, including blood and tissue, for nine major classes of drugs.

Authors+Show Affiliations

Armed Forces Institute of Pathology, Division of Forensic Toxicology, Washington, DC 20306-6000, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10664895

Citation

Moore, K A., et al. "Screening Postmortem Blood and Tissues for Nine Classes [correction of Cases] of Drugs of Abuse Using Automated Microplate Immunoassay." Forensic Science International, vol. 106, no. 2, 1999, pp. 93-102.
Moore KA, Werner C, Zannelli RM, et al. Screening postmortem blood and tissues for nine classes [correction of cases] of drugs of abuse using automated microplate immunoassay. Forensic Sci Int. 1999;106(2):93-102.
Moore, K. A., Werner, C., Zannelli, R. M., Levine, B., & Smith, M. L. (1999). Screening postmortem blood and tissues for nine classes [correction of cases] of drugs of abuse using automated microplate immunoassay. Forensic Science International, 106(2), pp. 93-102.
Moore KA, et al. Screening Postmortem Blood and Tissues for Nine Classes [correction of Cases] of Drugs of Abuse Using Automated Microplate Immunoassay. Forensic Sci Int. 1999 Dec 6;106(2):93-102. PubMed PMID: 10664895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screening postmortem blood and tissues for nine classes [correction of cases] of drugs of abuse using automated microplate immunoassay. AU - Moore,K A, AU - Werner,C, AU - Zannelli,R M, AU - Levine,B, AU - Smith,M L, PY - 2000/2/9/pubmed PY - 2000/2/26/medline PY - 2000/2/9/entrez SP - 93 EP - 102 JF - Forensic science international JO - Forensic Sci. Int. VL - 106 IS - 2 N2 - A commercially available enzyme-linked immunosorbant assay (ELISA) was evaluated as a screening procedure for the detection of nine classes of abused drugs in postmortem blood and tissue specimens. Specifically, postmortem blood, fluid and/or tissue homogenates were screened for amphetamine (AMP), methamphetamine (MET), barbiturates (BARB), benzodiazepines (BZD), cannabinoids (CNB), cocaine (benzoylecgonine; BE), morphine-specific (MOR), opiates (class; OPI), phencyclidine (PCP) and lysergic acid diethylamide (LSD) by ELISA and by coated tube radioimmunoassay (CTR) (BARB, BE, OPI, PCP, LSD) or double-antibody radioimmunoassay (DAR) (AMP/METH, BZD, CNB). Specimens that screened 'positive' by any method were confirmed and quantitated by gas chromatography/mass spectrometry (GC/MS). The only assay that appeared to perform less optimally than RIA was the MOR assay (five false negatives). However, this assay is very specific for free morphine while the GC/MS confirmation method provided a total morphine value. The OPI assay was more sensitive, producing fewer false negatives, and is recommended for broad class opiate screening. EIA is an adequate alternative to RIA for screening postmortem specimens, including blood and tissue, for nine major classes of drugs. SN - 0379-0738 UR - https://www.unboundmedicine.com/medline/citation/10664895/Screening_postmortem_blood_and_tissues_for_nine_classes_[correction_of_cases]_of_drugs_of_abuse_using_automated_microplate_immunoassay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379073899001826 DB - PRIME DP - Unbound Medicine ER -