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Cpx two-component signal transduction in Escherichia coli: excessive CpxR-P levels underlie CpxA* phenotypes.
J Bacteriol. 2000 Mar; 182(5):1423-6.JB

Abstract

In Escherichia coli, the CpxA-CpxR two-component signal transduction system and the sigma(E) and sigma(32) response pathways jointly regulate gene expression in adaptation to adverse conditions. These include envelope protein distress, heat shock, oxidative stress, high pH, and entry into stationary phase. Certain mutant versions of the CpxA sensor protein (CpxA* proteins) exhibit an elevated ratio of kinase to phosphatase activity on CpxR, the cognate response regulator. As a result, CpxA* strains display numerous phenotypes, many of which cannot be easily related to currently known functions of the CpxA-CpxR pathway. It is unclear whether CpxA* phenotypes are caused solely by hyperphosphorylation of CpxR. We here report that all of the tested CpxA* phenotypes depend on elevated levels of CpxR-P and not on cross-signalling of CpxA* to noncognate response regulators.

Authors+Show Affiliations

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10671468

Citation

De Wulf, P, and E C. Lin. "Cpx Two-component Signal Transduction in Escherichia Coli: Excessive CpxR-P Levels Underlie CpxA* Phenotypes." Journal of Bacteriology, vol. 182, no. 5, 2000, pp. 1423-6.
De Wulf P, Lin EC. Cpx two-component signal transduction in Escherichia coli: excessive CpxR-P levels underlie CpxA* phenotypes. J Bacteriol. 2000;182(5):1423-6.
De Wulf, P., & Lin, E. C. (2000). Cpx two-component signal transduction in Escherichia coli: excessive CpxR-P levels underlie CpxA* phenotypes. Journal of Bacteriology, 182(5), 1423-6.
De Wulf P, Lin EC. Cpx Two-component Signal Transduction in Escherichia Coli: Excessive CpxR-P Levels Underlie CpxA* Phenotypes. J Bacteriol. 2000;182(5):1423-6. PubMed PMID: 10671468.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cpx two-component signal transduction in Escherichia coli: excessive CpxR-P levels underlie CpxA* phenotypes. AU - De Wulf,P, AU - Lin,E C, PY - 2000/2/15/pubmed PY - 2000/3/4/medline PY - 2000/2/15/entrez SP - 1423 EP - 6 JF - Journal of bacteriology JO - J Bacteriol VL - 182 IS - 5 N2 - In Escherichia coli, the CpxA-CpxR two-component signal transduction system and the sigma(E) and sigma(32) response pathways jointly regulate gene expression in adaptation to adverse conditions. These include envelope protein distress, heat shock, oxidative stress, high pH, and entry into stationary phase. Certain mutant versions of the CpxA sensor protein (CpxA* proteins) exhibit an elevated ratio of kinase to phosphatase activity on CpxR, the cognate response regulator. As a result, CpxA* strains display numerous phenotypes, many of which cannot be easily related to currently known functions of the CpxA-CpxR pathway. It is unclear whether CpxA* phenotypes are caused solely by hyperphosphorylation of CpxR. We here report that all of the tested CpxA* phenotypes depend on elevated levels of CpxR-P and not on cross-signalling of CpxA* to noncognate response regulators. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/10671468/Cpx_two_component_signal_transduction_in_Escherichia_coli:_excessive_CpxR_P_levels_underlie_CpxA__phenotypes_ L2 - https://journals.asm.org/doi/10.1128/JB.182.5.1423-1426.2000?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -