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Horse IgG isotypes and cross-neutralization of two snake antivenoms produced in Brazil and Costa Rica.
Toxicon. 2000 May; 38(5):633-44.T

Abstract

Horse IgG isotypes and cross-neutralization of two snake antivenoms produced in Brazil and Costa Rica. Toxicon 000-000. This work compared the specificity, ELISA titers and IgG subclass content of the polyvalent antivenom (anti-Bothrops asper, Crotalus durissus durissus and Lachesis muta stenophrys) of Instituto Clodomiro Picado (Costa Rica) and the bothropic antivenom (anti-Bothrops jararaca, B. jararacussu, B. moojeni, B. neuwiedi and B. alternatus) of Instituto Butantan (Brazil). The role of IgG(T) and IgGa subclasses in neutralization of some venom toxic activities and the cross neutralization of the antivenoms against B. jararaca and B. asper venoms were also evaluated. Both antivenoms were able to recognize B. asper and B. jararaca venoms by immunoblotting and presented similar antibody titers when assayed by ELISA. IgG(T) was highest, followed by IgGa, IgGb and IgGc. IgGa and IgG(T) isotypes isolated from both antivenoms by affinity chromatography were tested for neutralization of lethal, hemorrhagic, coagulant and phospholipase A2 activities of the homologous venoms. In both antivenoms, IgG(T) was the major isotype responsible for neutralization of all the tested activities, followed by IgGa. These results suggest that Instituto Butantan and Instituto Clodomiro Picado antivenoms have the same IgG profile and their neutralizing ability is due mostly to the IgG(T) isotype. Also, they neutralize lethality in mice induced by homologous and heterologous venoms, the bothropic antivenom of Instituto Butantan being more effective.

Authors+Show Affiliations

Laboratório de Imunopatologia, Instituto Butantan, São Paulo, Brazil. irenefer@usp.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10673156

Citation

Fernandes, I, et al. "Horse IgG Isotypes and Cross-neutralization of Two Snake Antivenoms Produced in Brazil and Costa Rica." Toxicon : Official Journal of the International Society On Toxinology, vol. 38, no. 5, 2000, pp. 633-44.
Fernandes I, Lima EX, Takehara HA, et al. Horse IgG isotypes and cross-neutralization of two snake antivenoms produced in Brazil and Costa Rica. Toxicon. 2000;38(5):633-44.
Fernandes, I., Lima, E. X., Takehara, H. A., Moura-da-Silva, A. M., Tanjoni, I., & Gutiérrez, J. M. (2000). Horse IgG isotypes and cross-neutralization of two snake antivenoms produced in Brazil and Costa Rica. Toxicon : Official Journal of the International Society On Toxinology, 38(5), 633-44.
Fernandes I, et al. Horse IgG Isotypes and Cross-neutralization of Two Snake Antivenoms Produced in Brazil and Costa Rica. Toxicon. 2000;38(5):633-44. PubMed PMID: 10673156.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Horse IgG isotypes and cross-neutralization of two snake antivenoms produced in Brazil and Costa Rica. AU - Fernandes,I, AU - Lima,E X, AU - Takehara,H A, AU - Moura-da-Silva,A M, AU - Tanjoni,I, AU - Gutiérrez,J M, PY - 2000/2/15/pubmed PY - 2000/4/1/medline PY - 2000/2/15/entrez SP - 633 EP - 44 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 38 IS - 5 N2 - Horse IgG isotypes and cross-neutralization of two snake antivenoms produced in Brazil and Costa Rica. Toxicon 000-000. This work compared the specificity, ELISA titers and IgG subclass content of the polyvalent antivenom (anti-Bothrops asper, Crotalus durissus durissus and Lachesis muta stenophrys) of Instituto Clodomiro Picado (Costa Rica) and the bothropic antivenom (anti-Bothrops jararaca, B. jararacussu, B. moojeni, B. neuwiedi and B. alternatus) of Instituto Butantan (Brazil). The role of IgG(T) and IgGa subclasses in neutralization of some venom toxic activities and the cross neutralization of the antivenoms against B. jararaca and B. asper venoms were also evaluated. Both antivenoms were able to recognize B. asper and B. jararaca venoms by immunoblotting and presented similar antibody titers when assayed by ELISA. IgG(T) was highest, followed by IgGa, IgGb and IgGc. IgGa and IgG(T) isotypes isolated from both antivenoms by affinity chromatography were tested for neutralization of lethal, hemorrhagic, coagulant and phospholipase A2 activities of the homologous venoms. In both antivenoms, IgG(T) was the major isotype responsible for neutralization of all the tested activities, followed by IgGa. These results suggest that Instituto Butantan and Instituto Clodomiro Picado antivenoms have the same IgG profile and their neutralizing ability is due mostly to the IgG(T) isotype. Also, they neutralize lethality in mice induced by homologous and heterologous venoms, the bothropic antivenom of Instituto Butantan being more effective. SN - 0041-0101 UR - https://www.unboundmedicine.com/medline/citation/10673156/Horse_IgG_isotypes_and_cross_neutralization_of_two_snake_antivenoms_produced_in_Brazil_and_Costa_Rica_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(99)00177-4 DB - PRIME DP - Unbound Medicine ER -