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Cloning and characterization of an alpha-neurotoxin-type protein specific for the coral snake Micrurus corallinus.
Biochem Biophys Res Commun. 2000 Jan 27; 267(3):887-91.BB

Abstract

During the cloning of abundant cDNAs expressed in the Micrurus corallinus coral snake venom gland, we cloned an alpha-neurotoxin homologue cDNA (nxh1). Two others isoforms were also cloned (nxh3 and nxh7, respectively). The nxh1 cDNA codes for a potential coral snake toxin with a signal peptide of 21 amino acids plus a predicted mature peptide with 57 amino acids. The deduced protein is highly similar to known toxic three-finger alpha-neurotoxins, with four deduced S-S bridges at the same conserved positions. This is the first cDNA coding for a three-finger related protein described so far for coral snakes. However, the predicted protein does not possess some of the important amino acids for the nicotinic acetylcholine receptor interaction. This protein was expressed in Escherichia coli as a His-tagged protein that allowed the rapid purification of the recombinant protein. This protein was used to generate antibodies which recognized the recombinant protein in Western blot and also a single band present in the M. corallinus venom, but not in the venom of 10 other Micrurus species.

Authors+Show Affiliations

Centro de Biotecnologia, Instituto Butantan, São Paulo, SP, 05503-900, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10673386

Citation

Silveira de Oliveira, J, et al. "Cloning and Characterization of an Alpha-neurotoxin-type Protein Specific for the Coral Snake Micrurus Corallinus." Biochemical and Biophysical Research Communications, vol. 267, no. 3, 2000, pp. 887-91.
Silveira de Oliveira J, Rossan de Brandão Prieto da Silva A, Soares MB, et al. Cloning and characterization of an alpha-neurotoxin-type protein specific for the coral snake Micrurus corallinus. Biochem Biophys Res Commun. 2000;267(3):887-91.
Silveira de Oliveira, J., Rossan de Brandão Prieto da Silva, A., Soares, M. B., Stephano, M. A., de Oliveira Dias, W., Raw, I., & Ho, P. L. (2000). Cloning and characterization of an alpha-neurotoxin-type protein specific for the coral snake Micrurus corallinus. Biochemical and Biophysical Research Communications, 267(3), 887-91.
Silveira de Oliveira J, et al. Cloning and Characterization of an Alpha-neurotoxin-type Protein Specific for the Coral Snake Micrurus Corallinus. Biochem Biophys Res Commun. 2000 Jan 27;267(3):887-91. PubMed PMID: 10673386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and characterization of an alpha-neurotoxin-type protein specific for the coral snake Micrurus corallinus. AU - Silveira de Oliveira,J, AU - Rossan de Brandão Prieto da Silva,A, AU - Soares,M B, AU - Stephano,M A, AU - de Oliveira Dias,W, AU - Raw,I, AU - Ho,P L, PY - 2000/2/16/pubmed PY - 2000/3/11/medline PY - 2000/2/16/entrez SP - 887 EP - 91 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 267 IS - 3 N2 - During the cloning of abundant cDNAs expressed in the Micrurus corallinus coral snake venom gland, we cloned an alpha-neurotoxin homologue cDNA (nxh1). Two others isoforms were also cloned (nxh3 and nxh7, respectively). The nxh1 cDNA codes for a potential coral snake toxin with a signal peptide of 21 amino acids plus a predicted mature peptide with 57 amino acids. The deduced protein is highly similar to known toxic three-finger alpha-neurotoxins, with four deduced S-S bridges at the same conserved positions. This is the first cDNA coding for a three-finger related protein described so far for coral snakes. However, the predicted protein does not possess some of the important amino acids for the nicotinic acetylcholine receptor interaction. This protein was expressed in Escherichia coli as a His-tagged protein that allowed the rapid purification of the recombinant protein. This protein was used to generate antibodies which recognized the recombinant protein in Western blot and also a single band present in the M. corallinus venom, but not in the venom of 10 other Micrurus species. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/10673386/Cloning_and_characterization_of_an_alpha_neurotoxin_type_protein_specific_for_the_coral_snake_Micrurus_corallinus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006291X99920338 DB - PRIME DP - Unbound Medicine ER -