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Alopecia areata is a T-lymphocyte mediated autoimmune disease: lesional human T-lymphocytes transfer alopecia areata to human skin grafts on SCID mice.
J Investig Dermatol Symp Proc. 1999 Dec; 4(3):207-10.JI

Abstract

Much evidence suggests that alopecia areata is a tissue restricted autoimmune disease. Alopecia areata responds to immunosuppressive agents, and is associated with other tissue restricted autoimmune diseases, including autoimmune thyroiditis and vitiligo. Furthermore, hair regrows when involved scalp is transplanted to nude mice. This study was undertaken to determine whether alopecia areata is mediated by T lymphocytes. Involved scalp from alopecia areata patients was grafted onto SCID mice. Additional biopsies from lesional scalp of the same patients were used to isolate T lymphocytes. These T lymphocytes were cultured with hair follicle homogenate, as well as autologous antigen presenting cells. The T lymphocytes were then injected into autologous scalp grafts on the SCID mice, which had regrown hair. Injection of scalp T lymphocytes resulted in hair loss. Hair loss was associated with the histologic and immunochemical changes of alopecia areata, including perifollicular infiltrates of T cells, along with HLA-DR and ICAM-1 expression by the follicular epithelium. Scalp T lymphocytes that had not been cultured with hair follicle homogenate did not have this effect. Preliminary data suggests hair loss requires a collaboration between CD8+ and CD4+T cells. These studies have demonstrated that alopecia areata can be induced by the transfer of T cells that recognize a hair follicle autoantigen.

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Authors+Show Affiliations

Gilhar A
Skin Research Laboratory, The B. Rapaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Shalaginov R
No affiliation info available
Assy B
No affiliation info available
Serafimovich S
No affiliation info available
Kalish RS
No affiliation info available

MeSH

Adoptive TransferAlopecia AreataAnimalsAntigen PresentationAutoantigensAutoimmunityHumansMiceMice, SCIDSkinSkin TransplantationT-LymphocytesTransplantation, Heterologous

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10674367

Citation

Gilhar, A, et al. "Alopecia Areata Is a T-lymphocyte Mediated Autoimmune Disease: Lesional Human T-lymphocytes Transfer Alopecia Areata to Human Skin Grafts On SCID Mice." The Journal of Investigative Dermatology. Symposium Proceedings, vol. 4, no. 3, 1999, pp. 207-10.
Gilhar A, Shalaginov R, Assy B, et al. Alopecia areata is a T-lymphocyte mediated autoimmune disease: lesional human T-lymphocytes transfer alopecia areata to human skin grafts on SCID mice. J Investig Dermatol Symp Proc. 1999;4(3):207-10.
Gilhar, A., Shalaginov, R., Assy, B., Serafimovich, S., & Kalish, R. S. (1999). Alopecia areata is a T-lymphocyte mediated autoimmune disease: lesional human T-lymphocytes transfer alopecia areata to human skin grafts on SCID mice. The Journal of Investigative Dermatology. Symposium Proceedings, 4(3), 207-10.
Gilhar A, et al. Alopecia Areata Is a T-lymphocyte Mediated Autoimmune Disease: Lesional Human T-lymphocytes Transfer Alopecia Areata to Human Skin Grafts On SCID Mice. J Investig Dermatol Symp Proc. 1999;4(3):207-10. PubMed PMID: 10674367.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alopecia areata is a T-lymphocyte mediated autoimmune disease: lesional human T-lymphocytes transfer alopecia areata to human skin grafts on SCID mice. AU - Gilhar,A, AU - Shalaginov,R, AU - Assy,B, AU - Serafimovich,S, AU - Kalish,R S, PY - 2000/2/16/pubmed PY - 2000/3/18/medline PY - 2000/2/16/entrez SP - 207 EP - 10 JF - The journal of investigative dermatology. Symposium proceedings JO - J Investig Dermatol Symp Proc VL - 4 IS - 3 N2 - Much evidence suggests that alopecia areata is a tissue restricted autoimmune disease. Alopecia areata responds to immunosuppressive agents, and is associated with other tissue restricted autoimmune diseases, including autoimmune thyroiditis and vitiligo. Furthermore, hair regrows when involved scalp is transplanted to nude mice. This study was undertaken to determine whether alopecia areata is mediated by T lymphocytes. Involved scalp from alopecia areata patients was grafted onto SCID mice. Additional biopsies from lesional scalp of the same patients were used to isolate T lymphocytes. These T lymphocytes were cultured with hair follicle homogenate, as well as autologous antigen presenting cells. The T lymphocytes were then injected into autologous scalp grafts on the SCID mice, which had regrown hair. Injection of scalp T lymphocytes resulted in hair loss. Hair loss was associated with the histologic and immunochemical changes of alopecia areata, including perifollicular infiltrates of T cells, along with HLA-DR and ICAM-1 expression by the follicular epithelium. Scalp T lymphocytes that had not been cultured with hair follicle homogenate did not have this effect. Preliminary data suggests hair loss requires a collaboration between CD8+ and CD4+T cells. These studies have demonstrated that alopecia areata can be induced by the transfer of T cells that recognize a hair follicle autoantigen. SN - 1087-0024 UR - https://www.unboundmedicine.com/medline/citation/10674367/Alopecia_areata_is_a_T_lymphocyte_mediated_autoimmune_disease:_lesional_human_T_lymphocytes_transfer_alopecia_areata_to_human_skin_grafts_on_SCID_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1087-0024(15)30265-3 DB - PRIME DP - Unbound Medicine ER -