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Confirmation testing of amphetamines and designer drugs in human urine by capillary electrophoresis-ion trap mass spectrometry.
Electrophoresis. 2000 Jan; 21(2):380-7.E

Abstract

Monitoring of amphetamines and designer drugs in human urine is a timely topic in clinical toxicology, surveillance of drug substitution, forensic science, drug testing at the workplace, and doping control. Confirmation testing of urinary amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy) and 3,4-methylenedioxyamphetamine (MDA) by capillary electrophoresis (CE) combined with atmospheric pressure electrospray ionization and ion trap mass spectrometry (MS) is described. Using an aqueous pH 4.6 buffer composed of ammonium acetate/acetic acid, CE-MS and CE-MS2 provided data that permitted the unambiguous confirmation of these drugs in external quality control urines. Furthermore, other drugs of abuse present in alkaline urinary extracts, including methadone and morphine, could also be monitored. The data presented illustrate that the sensitivity achieved with the benchtop MS is comparable to that observed by CE with UV absorption detection. CE-MS2 is further shown to be capable of identifying comigrating compounds, including the comigration of amphetamine with nicotine.

Authors+Show Affiliations

Department of Clinical Pharmacology, University of Bern, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10675019

Citation

Ramseier, A, et al. "Confirmation Testing of Amphetamines and Designer Drugs in Human Urine By Capillary Electrophoresis-ion Trap Mass Spectrometry." Electrophoresis, vol. 21, no. 2, 2000, pp. 380-7.
Ramseier A, Siethoff C, Caslavska J, et al. Confirmation testing of amphetamines and designer drugs in human urine by capillary electrophoresis-ion trap mass spectrometry. Electrophoresis. 2000;21(2):380-7.
Ramseier, A., Siethoff, C., Caslavska, J., & Thormann, W. (2000). Confirmation testing of amphetamines and designer drugs in human urine by capillary electrophoresis-ion trap mass spectrometry. Electrophoresis, 21(2), 380-7.
Ramseier A, et al. Confirmation Testing of Amphetamines and Designer Drugs in Human Urine By Capillary Electrophoresis-ion Trap Mass Spectrometry. Electrophoresis. 2000;21(2):380-7. PubMed PMID: 10675019.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Confirmation testing of amphetamines and designer drugs in human urine by capillary electrophoresis-ion trap mass spectrometry. AU - Ramseier,A, AU - Siethoff,C, AU - Caslavska,J, AU - Thormann,W, PY - 2000/2/16/pubmed PY - 2000/3/18/medline PY - 2000/2/16/entrez SP - 380 EP - 7 JF - Electrophoresis JO - Electrophoresis VL - 21 IS - 2 N2 - Monitoring of amphetamines and designer drugs in human urine is a timely topic in clinical toxicology, surveillance of drug substitution, forensic science, drug testing at the workplace, and doping control. Confirmation testing of urinary amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA, Ecstasy) and 3,4-methylenedioxyamphetamine (MDA) by capillary electrophoresis (CE) combined with atmospheric pressure electrospray ionization and ion trap mass spectrometry (MS) is described. Using an aqueous pH 4.6 buffer composed of ammonium acetate/acetic acid, CE-MS and CE-MS2 provided data that permitted the unambiguous confirmation of these drugs in external quality control urines. Furthermore, other drugs of abuse present in alkaline urinary extracts, including methadone and morphine, could also be monitored. The data presented illustrate that the sensitivity achieved with the benchtop MS is comparable to that observed by CE with UV absorption detection. CE-MS2 is further shown to be capable of identifying comigrating compounds, including the comigration of amphetamine with nicotine. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/10675019/Confirmation_testing_of_amphetamines_and_designer_drugs_in_human_urine_by_capillary_electrophoresis_ion_trap_mass_spectrometry_ L2 - https://doi.org/10.1002/(SICI)1522-2683(20000101)21:2<380::AID-ELPS380>3.0.CO;2-L DB - PRIME DP - Unbound Medicine ER -