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Two-dimensional gel electrophoresis using immobilized pH gradient tube gels.
Electrophoresis. 2000 Jan; 21(2):440-5.E

Abstract

An apparatus for the preparation of gels for immobilized pH gradient isoelectric focusing (IPG) in glass tubes was developed. Using this apparatus, the highly reproducible immobilized pH gradient can be formed with Immobilines in polyacrylamide gels, and IPG gels at all possible pH ranges can be easily prepared at low cost. The IPG tube gels in the first dimension in two-dimensional gel electrophoresis was used to separate and identify a number of rice embryo proteins in the proteome analysis. There was no difference in resolution of proteins between the tube gels and the commercially available slab gels; after electrophoresis, however, we could efficiently obtain a larger amount of the purified proteins from the tube gels than from the slab gels.

Authors+Show Affiliations

Yokohama City University, Kihara Institute for Biological Research/Graduate School of Integrated Science, Totsuka, Japan. hirano@yokohama-cu.ac.jpNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10675025

Citation

Hirano, H, et al. "Two-dimensional Gel Electrophoresis Using Immobilized pH Gradient Tube Gels." Electrophoresis, vol. 21, no. 2, 2000, pp. 440-5.
Hirano H, Kawasaki H, Sassa H. Two-dimensional gel electrophoresis using immobilized pH gradient tube gels. Electrophoresis. 2000;21(2):440-5.
Hirano, H., Kawasaki, H., & Sassa, H. (2000). Two-dimensional gel electrophoresis using immobilized pH gradient tube gels. Electrophoresis, 21(2), 440-5.
Hirano H, Kawasaki H, Sassa H. Two-dimensional Gel Electrophoresis Using Immobilized pH Gradient Tube Gels. Electrophoresis. 2000;21(2):440-5. PubMed PMID: 10675025.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two-dimensional gel electrophoresis using immobilized pH gradient tube gels. AU - Hirano,H, AU - Kawasaki,H, AU - Sassa,H, PY - 2000/2/16/pubmed PY - 2000/3/18/medline PY - 2000/2/16/entrez SP - 440 EP - 5 JF - Electrophoresis JO - Electrophoresis VL - 21 IS - 2 N2 - An apparatus for the preparation of gels for immobilized pH gradient isoelectric focusing (IPG) in glass tubes was developed. Using this apparatus, the highly reproducible immobilized pH gradient can be formed with Immobilines in polyacrylamide gels, and IPG gels at all possible pH ranges can be easily prepared at low cost. The IPG tube gels in the first dimension in two-dimensional gel electrophoresis was used to separate and identify a number of rice embryo proteins in the proteome analysis. There was no difference in resolution of proteins between the tube gels and the commercially available slab gels; after electrophoresis, however, we could efficiently obtain a larger amount of the purified proteins from the tube gels than from the slab gels. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/10675025/Two_dimensional_gel_electrophoresis_using_immobilized_pH_gradient_tube_gels_ DB - PRIME DP - Unbound Medicine ER -