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Quantitative analysis of clenbuterol in meat products using liquid chromatography-electrospray ionisation tandem mass spectrometry.
J Chromatogr B Biomed Sci Appl. 1999 Dec 24; 736(1-2):209-19.JC

Abstract

A method is presented that allows quantitation of clenbuterol in meat and liver products at the ng/kg level by liquid chromatography-electrospray ionisation tandem mass spectrometry (LC-ESIMS-MS) using a stable isotopically labeled internal standard. The practical procedure involves acid extraction followed by two solid-phase clean-up steps with C18 and strong cation-exchange (SCX) resins. The typical recovery of the analyte spiked at 0.4 microg/kg in meat and liver samples was at 63+/-7%. Mass spectral acquisition was done in multiple reaction monitoring (MRM) to provide a high degree of sensitivity, achieving a limit of detection and quantitation at 10 and 15 ng/kg, respectively. Two precursor ions at m/z 277 and 279, corresponding to the characteristic isotopic cluster of the two chlorine atoms of clenbuterol, were monitored by LC-ESIMS-MS to provide unambiguous identity of the analyte. Samples of meat and liver of various origins with either incurred residues or spiked with known amounts of clenbuterol were used to validate the method.

Authors+Show Affiliations

Department of Quality and Safety Assurance, Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10677001

Citation

Guy, P A., et al. "Quantitative Analysis of Clenbuterol in Meat Products Using Liquid Chromatography-electrospray Ionisation Tandem Mass Spectrometry." Journal of Chromatography. B, Biomedical Sciences and Applications, vol. 736, no. 1-2, 1999, pp. 209-19.
Guy PA, Savoy MC, Stadler RH. Quantitative analysis of clenbuterol in meat products using liquid chromatography-electrospray ionisation tandem mass spectrometry. J Chromatogr B Biomed Sci Appl. 1999;736(1-2):209-19.
Guy, P. A., Savoy, M. C., & Stadler, R. H. (1999). Quantitative analysis of clenbuterol in meat products using liquid chromatography-electrospray ionisation tandem mass spectrometry. Journal of Chromatography. B, Biomedical Sciences and Applications, 736(1-2), 209-19.
Guy PA, Savoy MC, Stadler RH. Quantitative Analysis of Clenbuterol in Meat Products Using Liquid Chromatography-electrospray Ionisation Tandem Mass Spectrometry. J Chromatogr B Biomed Sci Appl. 1999 Dec 24;736(1-2):209-19. PubMed PMID: 10677001.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative analysis of clenbuterol in meat products using liquid chromatography-electrospray ionisation tandem mass spectrometry. AU - Guy,P A, AU - Savoy,M C, AU - Stadler,R H, PY - 2000/2/17/pubmed PY - 2000/2/17/medline PY - 2000/2/17/entrez SP - 209 EP - 19 JF - Journal of chromatography. B, Biomedical sciences and applications JO - J. Chromatogr. B Biomed. Sci. Appl. VL - 736 IS - 1-2 N2 - A method is presented that allows quantitation of clenbuterol in meat and liver products at the ng/kg level by liquid chromatography-electrospray ionisation tandem mass spectrometry (LC-ESIMS-MS) using a stable isotopically labeled internal standard. The practical procedure involves acid extraction followed by two solid-phase clean-up steps with C18 and strong cation-exchange (SCX) resins. The typical recovery of the analyte spiked at 0.4 microg/kg in meat and liver samples was at 63+/-7%. Mass spectral acquisition was done in multiple reaction monitoring (MRM) to provide a high degree of sensitivity, achieving a limit of detection and quantitation at 10 and 15 ng/kg, respectively. Two precursor ions at m/z 277 and 279, corresponding to the characteristic isotopic cluster of the two chlorine atoms of clenbuterol, were monitored by LC-ESIMS-MS to provide unambiguous identity of the analyte. Samples of meat and liver of various origins with either incurred residues or spiked with known amounts of clenbuterol were used to validate the method. SN - 1387-2273 UR - https://www.unboundmedicine.com/medline/citation/10677001/Quantitative_analysis_of_clenbuterol_in_meat_products_using_liquid_chromatography_electrospray_ionisation_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -