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Inhibitory effects of estradiol on inward rectifier and delayed rectifier K+ currents in guinea pig ventricular myocytes.
Zhongguo Yao Li Xue Bao. 1999 Jul; 20(7):631-4.ZY

Abstract

AIM

To study the effects of estradiol (Est) on inward rectifier K+ (IK1) and delayed rectifier K+ (IK) channels in isolated guinea pig ventricular myocytes.

METHODS

Using whole cell patch-clamp recording techniques.

RESULTS

Est 10 mumol.L-1 and 100 mumol.L-1 decreased the action potential duration, APD50, from (474 +/- 71) ms to (330 +/- 75) ms and (229 +/- 67) ms (n = 7 cells of 7 guinea pigs, P < 0.05), respectively. Est 100 mumol.L-1 also decreased APD90 from (587 +/- 60) ms to (418 +/- 79) ms (n = 7, P < 0.05). Est inhibited IK tail current (IK.tail) concentration-dependently. IK.tail was depressed 53% (n = 5, P < 0.05) at 10 mumol.L-1 and 80% (n = 5, P < 0.01) at 100 mumol.L-1 compared with control. Est > or = 10 mumol.L-1 blocked IK1. The maximal inhibition of inward current of IK1 occurred at -100 mV test potential was 49% (n = 5, P < 0.01) and outward current of IK1 at -40 mV was 72% (n = 5, P < 0.01). The reverse potential shifted negatively, from -70 to -76 mV.

CONCLUSION

Est possessed blocking effects on both IK1 and IK channels in guinea pig ventricular myocytes.

Authors+Show Affiliations

Shanghai Institute of Physiology, Chinese Academy of Sciences, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10678128

Citation

Zhang, Y, et al. "Inhibitory Effects of Estradiol On Inward Rectifier and Delayed Rectifier K+ Currents in Guinea Pig Ventricular Myocytes." Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica, vol. 20, no. 7, 1999, pp. 631-4.
Zhang Y, Song LL, Gu SZ, et al. Inhibitory effects of estradiol on inward rectifier and delayed rectifier K+ currents in guinea pig ventricular myocytes. Zhongguo Yao Li Xue Bao. 1999;20(7):631-4.
Zhang, Y., Song, L. L., Gu, S. Z., Lu, S. G., & Zhou, Z. N. (1999). Inhibitory effects of estradiol on inward rectifier and delayed rectifier K+ currents in guinea pig ventricular myocytes. Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica, 20(7), 631-4.
Zhang Y, et al. Inhibitory Effects of Estradiol On Inward Rectifier and Delayed Rectifier K+ Currents in Guinea Pig Ventricular Myocytes. Zhongguo Yao Li Xue Bao. 1999;20(7):631-4. PubMed PMID: 10678128.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effects of estradiol on inward rectifier and delayed rectifier K+ currents in guinea pig ventricular myocytes. AU - Zhang,Y, AU - Song,L L, AU - Gu,S Z, AU - Lu,S G, AU - Zhou,Z N, PY - 2000/5/16/pubmed PY - 2000/5/16/medline PY - 2000/5/16/entrez SP - 631 EP - 4 JF - Zhongguo yao li xue bao = Acta pharmacologica Sinica JO - Zhongguo Yao Li Xue Bao VL - 20 IS - 7 N2 - AIM: To study the effects of estradiol (Est) on inward rectifier K+ (IK1) and delayed rectifier K+ (IK) channels in isolated guinea pig ventricular myocytes. METHODS: Using whole cell patch-clamp recording techniques. RESULTS: Est 10 mumol.L-1 and 100 mumol.L-1 decreased the action potential duration, APD50, from (474 +/- 71) ms to (330 +/- 75) ms and (229 +/- 67) ms (n = 7 cells of 7 guinea pigs, P < 0.05), respectively. Est 100 mumol.L-1 also decreased APD90 from (587 +/- 60) ms to (418 +/- 79) ms (n = 7, P < 0.05). Est inhibited IK tail current (IK.tail) concentration-dependently. IK.tail was depressed 53% (n = 5, P < 0.05) at 10 mumol.L-1 and 80% (n = 5, P < 0.01) at 100 mumol.L-1 compared with control. Est > or = 10 mumol.L-1 blocked IK1. The maximal inhibition of inward current of IK1 occurred at -100 mV test potential was 49% (n = 5, P < 0.01) and outward current of IK1 at -40 mV was 72% (n = 5, P < 0.01). The reverse potential shifted negatively, from -70 to -76 mV. CONCLUSION: Est possessed blocking effects on both IK1 and IK channels in guinea pig ventricular myocytes. SN - 0253-9756 UR - https://www.unboundmedicine.com/medline/citation/10678128/Inhibitory_effects_of_estradiol_on_inward_rectifier_and_delayed_rectifier_K+_currents_in_guinea_pig_ventricular_myocytes_ L2 - http://www.chinaphar.com/1671-4083/20/631.pdf DB - PRIME DP - Unbound Medicine ER -