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Characterization of Erwinia chrysanthemi PY35 cel and pel gene existing in tandem and rapid identification of their gene products.
Biochem Biophys Res Commun. 2000 Feb 16; 268(2):420-5.BB

Abstract

Genomic DNA of the phytopathogenic Erwinia chrysanthemi PY35 was partially digested with Sau3AI, ligated into the BamHI site of pBluescript II SK+, and introduced into E. coli. One clone that was able to hydrolyse carboxymethylcellulose and polygalacturonic acid was selected. A 2.9 kb fragment containing the pelL1 gene (pPY300) and cel5Z gene (pPY401) in tandem was subcloned and sequenced. The pelL1 and cel5Z genes had open reading frames of 1,278 bp and 1,281 bp encoding 425 and 426 amino acid residues with calculated molecular weights of 45,649 Da and 46,473 Da, respectively. pelL1 and cel5Z carried a typical prokaryotic signal peptide of 24 and 41 amino acid residues, respectively. The apparent molecular masses of the proteins when expressed in E. coli cells were approximately 43 kDa (PelL1) and 42 kDa (Cel5Z) as assessed by PGA-SDS-PAGE and CMC-SDS-PAGE.

Authors+Show Affiliations

Department of Microbiological Engineering, Chinju National University, Chinju, 660-758, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10679220

Citation

Park, S R., et al. "Characterization of Erwinia Chrysanthemi PY35 Cel and Pel Gene Existing in Tandem and Rapid Identification of Their Gene Products." Biochemical and Biophysical Research Communications, vol. 268, no. 2, 2000, pp. 420-5.
Park SR, Kim MK, Kim JO, et al. Characterization of Erwinia chrysanthemi PY35 cel and pel gene existing in tandem and rapid identification of their gene products. Biochem Biophys Res Commun. 2000;268(2):420-5.
Park, S. R., Kim, M. K., Kim, J. O., Bae, D. W., Cho, S. J., Cho, Y. U., & Yun, H. D. (2000). Characterization of Erwinia chrysanthemi PY35 cel and pel gene existing in tandem and rapid identification of their gene products. Biochemical and Biophysical Research Communications, 268(2), 420-5.
Park SR, et al. Characterization of Erwinia Chrysanthemi PY35 Cel and Pel Gene Existing in Tandem and Rapid Identification of Their Gene Products. Biochem Biophys Res Commun. 2000 Feb 16;268(2):420-5. PubMed PMID: 10679220.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Erwinia chrysanthemi PY35 cel and pel gene existing in tandem and rapid identification of their gene products. AU - Park,S R, AU - Kim,M K, AU - Kim,J O, AU - Bae,D W, AU - Cho,S J, AU - Cho,Y U, AU - Yun,H D, PY - 2000/2/19/pubmed PY - 2000/3/18/medline PY - 2000/2/19/entrez SP - 420 EP - 5 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 268 IS - 2 N2 - Genomic DNA of the phytopathogenic Erwinia chrysanthemi PY35 was partially digested with Sau3AI, ligated into the BamHI site of pBluescript II SK+, and introduced into E. coli. One clone that was able to hydrolyse carboxymethylcellulose and polygalacturonic acid was selected. A 2.9 kb fragment containing the pelL1 gene (pPY300) and cel5Z gene (pPY401) in tandem was subcloned and sequenced. The pelL1 and cel5Z genes had open reading frames of 1,278 bp and 1,281 bp encoding 425 and 426 amino acid residues with calculated molecular weights of 45,649 Da and 46,473 Da, respectively. pelL1 and cel5Z carried a typical prokaryotic signal peptide of 24 and 41 amino acid residues, respectively. The apparent molecular masses of the proteins when expressed in E. coli cells were approximately 43 kDa (PelL1) and 42 kDa (Cel5Z) as assessed by PGA-SDS-PAGE and CMC-SDS-PAGE. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/10679220/Characterization_of_Erwinia_chrysanthemi_PY35_cel_and_pel_gene_existing_in_tandem_and_rapid_identification_of_their_gene_products_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(00)92137-5 DB - PRIME DP - Unbound Medicine ER -