Tags

Type your tag names separated by a space and hit enter

Evaluation of different measles IgG assays based on recombinant proteins using a panel of low-titre sera.
J Virol Methods. 2000 Feb; 84(2):191-200.JV

Abstract

During the WHO campaign to eradicate measles, accurate discrimination between immune and non-immune individuals will become increasingly important. Due to waning immunity in vaccinated populations, the performance of a measles IgG assay depends mainly on its ability to detect reliably seronegative individuals among many vaccinees with low antibody levels. New serological tests based on recombinant proteins detect only a fraction of the total measles virus (MV) specific antibodies. Therefore, several assays based on recombinant MV-haemagglutinin (ELISA and flow cytometry) or MV-fusion protein (flow cytometry) as well as neutralisatlon and haemagglutination test have been evaluated using a large panel of low-titre and negative sera. Since such an evaluation is highly dependent on threshold values for positivity, the receiver operating characteristic curve analysis was applied. The H-FACS and the H-ELISA showed the best performing characteristics (specificity: 97.4 and 96.1%, respectively; sensitivity: 88.1 and 89.6%, respectively) and may be an alternative to the neutralisation assay. The number of undefined/grey zone sera was significantly lower compared to a commercial whole virus-based ELISA and therefore fewer individuals would be vaccinated unnecessarily.

Links

Publisher Full Text

Authors+Show Affiliations

Hartter HK
Department of Immunology and WHO Collaborating Center for Measles, Laboratoire National de Santé, Luxembourg, Luxembourg.
de Swart RL
No affiliation info available
Hanses F
No affiliation info available
Vos HW
No affiliation info available
Bouche FB
No affiliation info available
Osterhaus AD
No affiliation info available
Schneider F
No affiliation info available
Muller CP
No affiliation info available

MeSH

Antibodies, ViralEnzyme-Linked Immunosorbent AssayEvaluation Studies as TopicFlow CytometryHemagglutination TestsHemagglutininsHumansImmunoglobulin GMeaslesMeasles virusNeutralization TestsROC CurveRecombinant ProteinsReference StandardsSensitivity and Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10680969

Citation

Hartter, H K., et al. "Evaluation of Different Measles IgG Assays Based On Recombinant Proteins Using a Panel of Low-titre Sera." Journal of Virological Methods, vol. 84, no. 2, 2000, pp. 191-200.
Hartter HK, de Swart RL, Hanses F, et al. Evaluation of different measles IgG assays based on recombinant proteins using a panel of low-titre sera. J Virol Methods. 2000;84(2):191-200.
Hartter, H. K., de Swart, R. L., Hanses, F., Vos, H. W., Bouche, F. B., Osterhaus, A. D., Schneider, F., & Muller, C. P. (2000). Evaluation of different measles IgG assays based on recombinant proteins using a panel of low-titre sera. Journal of Virological Methods, 84(2), 191-200.
Hartter HK, et al. Evaluation of Different Measles IgG Assays Based On Recombinant Proteins Using a Panel of Low-titre Sera. J Virol Methods. 2000;84(2):191-200. PubMed PMID: 10680969.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of different measles IgG assays based on recombinant proteins using a panel of low-titre sera. AU - Hartter,H K, AU - de Swart,R L, AU - Hanses,F, AU - Vos,H W, AU - Bouche,F B, AU - Osterhaus,A D, AU - Schneider,F, AU - Muller,C P, PY - 2000/2/19/pubmed PY - 2000/3/18/medline PY - 2000/2/19/entrez SP - 191 EP - 200 JF - Journal of virological methods JO - J Virol Methods VL - 84 IS - 2 N2 - During the WHO campaign to eradicate measles, accurate discrimination between immune and non-immune individuals will become increasingly important. Due to waning immunity in vaccinated populations, the performance of a measles IgG assay depends mainly on its ability to detect reliably seronegative individuals among many vaccinees with low antibody levels. New serological tests based on recombinant proteins detect only a fraction of the total measles virus (MV) specific antibodies. Therefore, several assays based on recombinant MV-haemagglutinin (ELISA and flow cytometry) or MV-fusion protein (flow cytometry) as well as neutralisatlon and haemagglutination test have been evaluated using a large panel of low-titre and negative sera. Since such an evaluation is highly dependent on threshold values for positivity, the receiver operating characteristic curve analysis was applied. The H-FACS and the H-ELISA showed the best performing characteristics (specificity: 97.4 and 96.1%, respectively; sensitivity: 88.1 and 89.6%, respectively) and may be an alternative to the neutralisation assay. The number of undefined/grey zone sera was significantly lower compared to a commercial whole virus-based ELISA and therefore fewer individuals would be vaccinated unnecessarily. SN - 0166-0934 UR - https://www.unboundmedicine.com/medline/citation/10680969/Evaluation_of_different_measles_IgG_assays_based_on_recombinant_proteins_using_a_panel_of_low_titre_sera_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166093499001433 DB - PRIME DP - Unbound Medicine ER -