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Immunocytochemical localization of lipocalin-type prostaglandin D synthase in the bull testis and epididymis and on ejaculated sperm.
Biol Reprod. 2000 Mar; 62(3):547-56.BR

Abstract

Previously, we identified a 26-kDa fertility-associated protein in bull seminal plasma as lipocalin-type prostaglandin D synthase. The objective of the present study was to immunohistochemically localize this enzyme to the various cell types within the bull testis and seven subsegments of the epididymis, and on ejaculated sperm in order to gain further insight into its potential function in male reproduction. In the testis, immunoperoxidase staining was localized within the elongating spermatids and Sertoli cells of the seminiferous tubules, varying with the stage of the spermatogenic cycle. The highest level of staining occurred during stages III-VII. The cuboidal epithelial cells of the rete testis and efferent ducts were also immunoreactive. Expression of lipocalin-type prostaglandin D synthase was not uniform in the seven epididymal subsegments, suggesting a possible role in sperm maturation. In all epididymal regions, expression was limited to the epithelial principal cells; no immunoreactivity was apparent in other cell types. Lipocalin-type prostaglandin D synthase was strikingly localized in the caput epididymidis, while moderate to weak staining was observed in the remainder of the epididymis. Droplets of reaction product observed within the lumen increased progressively from the caput to cauda. Using fluorescence microscopy, we also localized lipocalin-type prostaglandin D synthase to the apical ridge of the acrosome on ejaculated sperm.

Authors+Show Affiliations

Department of Dairy and Animal Science, J.O. Almquist Research Center, Pennsylvania State University, University Park, PA 16802, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

10684794

Citation

Gerena, R L., et al. "Immunocytochemical Localization of Lipocalin-type Prostaglandin D Synthase in the Bull Testis and Epididymis and On Ejaculated Sperm." Biology of Reproduction, vol. 62, no. 3, 2000, pp. 547-56.
Gerena RL, Irikura D, Eguchi N, et al. Immunocytochemical localization of lipocalin-type prostaglandin D synthase in the bull testis and epididymis and on ejaculated sperm. Biol Reprod. 2000;62(3):547-56.
Gerena, R. L., Irikura, D., Eguchi, N., Urade, Y., & Killian, G. J. (2000). Immunocytochemical localization of lipocalin-type prostaglandin D synthase in the bull testis and epididymis and on ejaculated sperm. Biology of Reproduction, 62(3), 547-56.
Gerena RL, et al. Immunocytochemical Localization of Lipocalin-type Prostaglandin D Synthase in the Bull Testis and Epididymis and On Ejaculated Sperm. Biol Reprod. 2000;62(3):547-56. PubMed PMID: 10684794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunocytochemical localization of lipocalin-type prostaglandin D synthase in the bull testis and epididymis and on ejaculated sperm. AU - Gerena,R L, AU - Irikura,D, AU - Eguchi,N, AU - Urade,Y, AU - Killian,G J, PY - 2000/2/24/pubmed PY - 2000/4/15/medline PY - 2000/2/24/entrez SP - 547 EP - 56 JF - Biology of reproduction JO - Biol Reprod VL - 62 IS - 3 N2 - Previously, we identified a 26-kDa fertility-associated protein in bull seminal plasma as lipocalin-type prostaglandin D synthase. The objective of the present study was to immunohistochemically localize this enzyme to the various cell types within the bull testis and seven subsegments of the epididymis, and on ejaculated sperm in order to gain further insight into its potential function in male reproduction. In the testis, immunoperoxidase staining was localized within the elongating spermatids and Sertoli cells of the seminiferous tubules, varying with the stage of the spermatogenic cycle. The highest level of staining occurred during stages III-VII. The cuboidal epithelial cells of the rete testis and efferent ducts were also immunoreactive. Expression of lipocalin-type prostaglandin D synthase was not uniform in the seven epididymal subsegments, suggesting a possible role in sperm maturation. In all epididymal regions, expression was limited to the epithelial principal cells; no immunoreactivity was apparent in other cell types. Lipocalin-type prostaglandin D synthase was strikingly localized in the caput epididymidis, while moderate to weak staining was observed in the remainder of the epididymis. Droplets of reaction product observed within the lumen increased progressively from the caput to cauda. Using fluorescence microscopy, we also localized lipocalin-type prostaglandin D synthase to the apical ridge of the acrosome on ejaculated sperm. SN - 0006-3363 UR - https://www.unboundmedicine.com/medline/citation/10684794/Immunocytochemical_localization_of_lipocalin_type_prostaglandin_D_synthase_in_the_bull_testis_and_epididymis_and_on_ejaculated_sperm_ DB - PRIME DP - Unbound Medicine ER -