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Incorporation and washout of orally administered n-3 fatty acid ethyl esters in different plasma lipid fractions.
Br J Nutr. 1999 Dec; 82(6):481-8.BJ

Abstract

The aim of the present study was to quantify the incorporation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) into plasma lipids after oral administration of n-3 fatty acid ethyl esters, since little is known about the rate and pattern of incorporation into plasma lipid fractions. In addition, we aimed to obtain preliminary information regarding EPA half-life, which is needed to establish an optimal dosing schedule. Five healthy volunteers ingested two 8.5 g doses of n-3 fatty acid ethyl esters daily for 7 d, supplying 6.0 g EPA/d and 5.3 g DHA/d. The fatty acid compositions of plasma phospholipids (PL), cholesteryl esters (CE) and triacylglycerols (TAG) were determined during supplementation and during a washout period of 7 d. Half-lives of EPA and DHA were calculated. The proportion of EPA in PL showed a 15-fold increase after 7 d (P < 0.001), while DHA showed a smaller increase (P < 0.01). In CE, EPA also increased (P < 0.05), while DHA did not increase at all. Remarkably, incorporation of DHA into TAG was even higher than that of EPA. Half-life of EPA in PL ranged from 1.63 to 2.31 d (mean 1.97 (SE 0.15) d), whereas mean half-life of EPA in CE was 3.27 (SE 0.56) d. In three subjects, washout of EPA and DHA from TAG seemed to follow a bi-exponential pattern, with a short half-life (< 1 d) in the initial phase and a half-life of several days in the second phase. In conclusion, EPA ethyl esters are rapidly incorporated into plasma lipids, especially into PL. The relatively long half-life of EPA in plasma would permit a dosing schedule with intervals of > or = 12 h in supplementation studies.

Authors+Show Affiliations

Institute of Internal Medicine II, Erasmus University of Rotterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10690163

Citation

Zuijdgeest-van Leeuwen, S D., et al. "Incorporation and Washout of Orally Administered N-3 Fatty Acid Ethyl Esters in Different Plasma Lipid Fractions." The British Journal of Nutrition, vol. 82, no. 6, 1999, pp. 481-8.
Zuijdgeest-van Leeuwen SD, Dagnelie PC, Rietveld T, et al. Incorporation and washout of orally administered n-3 fatty acid ethyl esters in different plasma lipid fractions. Br J Nutr. 1999;82(6):481-8.
Zuijdgeest-van Leeuwen, S. D., Dagnelie, P. C., Rietveld, T., van den Berg, J. W., & Wilson, J. H. (1999). Incorporation and washout of orally administered n-3 fatty acid ethyl esters in different plasma lipid fractions. The British Journal of Nutrition, 82(6), 481-8.
Zuijdgeest-van Leeuwen SD, et al. Incorporation and Washout of Orally Administered N-3 Fatty Acid Ethyl Esters in Different Plasma Lipid Fractions. Br J Nutr. 1999;82(6):481-8. PubMed PMID: 10690163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Incorporation and washout of orally administered n-3 fatty acid ethyl esters in different plasma lipid fractions. AU - Zuijdgeest-van Leeuwen,S D, AU - Dagnelie,P C, AU - Rietveld,T, AU - van den Berg,J W, AU - Wilson,J H, PY - 2000/2/26/pubmed PY - 2000/3/11/medline PY - 2000/2/26/entrez SP - 481 EP - 8 JF - The British journal of nutrition JO - Br J Nutr VL - 82 IS - 6 N2 - The aim of the present study was to quantify the incorporation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) into plasma lipids after oral administration of n-3 fatty acid ethyl esters, since little is known about the rate and pattern of incorporation into plasma lipid fractions. In addition, we aimed to obtain preliminary information regarding EPA half-life, which is needed to establish an optimal dosing schedule. Five healthy volunteers ingested two 8.5 g doses of n-3 fatty acid ethyl esters daily for 7 d, supplying 6.0 g EPA/d and 5.3 g DHA/d. The fatty acid compositions of plasma phospholipids (PL), cholesteryl esters (CE) and triacylglycerols (TAG) were determined during supplementation and during a washout period of 7 d. Half-lives of EPA and DHA were calculated. The proportion of EPA in PL showed a 15-fold increase after 7 d (P < 0.001), while DHA showed a smaller increase (P < 0.01). In CE, EPA also increased (P < 0.05), while DHA did not increase at all. Remarkably, incorporation of DHA into TAG was even higher than that of EPA. Half-life of EPA in PL ranged from 1.63 to 2.31 d (mean 1.97 (SE 0.15) d), whereas mean half-life of EPA in CE was 3.27 (SE 0.56) d. In three subjects, washout of EPA and DHA from TAG seemed to follow a bi-exponential pattern, with a short half-life (< 1 d) in the initial phase and a half-life of several days in the second phase. In conclusion, EPA ethyl esters are rapidly incorporated into plasma lipids, especially into PL. The relatively long half-life of EPA in plasma would permit a dosing schedule with intervals of > or = 12 h in supplementation studies. SN - 0007-1145 UR - https://www.unboundmedicine.com/medline/citation/10690163/Incorporation_and_washout_of_orally_administered_n_3_fatty_acid_ethyl_esters_in_different_plasma_lipid_fractions_ L2 - https://www.cambridge.org/core/product/identifier/S0007114599001737/type/journal_article DB - PRIME DP - Unbound Medicine ER -