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Growth factor mRNA and protein in preserved human amniotic membrane.
Curr Eye Res. 2000 Mar; 20(3):173-7.CE

Abstract

PURPOSE

To investigate the expression of growth factor mRNA and the level of growth factor protein in preserved human amniotic membrane (AM).

METHODS

RT-PCR was used to examine the expression of mRNA for eight growth factors (EGF, TGF-alpha, KGF, HGF, bFGF, TGF-beta1, -beta2, -beta3) and two growth factor receptors (KGFR and HGFR) in human AM preserved at -80 degrees C for one month. In addition, ELISAs were used to measure the protein concentrations of seven growth factors (EGF, TGF-alpha, KGF, HGF, bFGF, TGF-beta1, -beta2) in preserved human corneas and in AM both with and without amniotic epithelium.

RESULTS

RT-PCR revealed that human AM expresses mRNA for EGF, TGF-alpha, KGF, HGF, bFGF, TGF-beta1, -beta2, -beta3, KGFR and HGFR, while ELISAs showed that it contains EGF, TGF-alpha, KGF, HGF, bFGF, TGF-beta1, -beta2. AM without amniotic epithelium also contains all seven growth factors examined, however, in this tissue the protein levels of EGF, KGF, HGF and bFGF were found to be significantly lower than in native AM.

CONCLUSIONS

Preserved human AM expresses mRNAs for a number of growth factors and contains several growth factor proteins that might benefit epithelialization after AM transplantation. High levels of EGF, KGF, HGF and bFGF in AM with amniotic epithelium as compared to AM without amniotic epithelium suggest an epithelial origin for these growth factors. We feel that EGF, KGF and HGF in particular might play important roles in ocular surface wound healing after AM transplantation.

Authors+Show Affiliations

Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan. nkoizumi@eye.ophth.kpu-m.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10694891

Citation

Koizumi, N J., et al. "Growth Factor mRNA and Protein in Preserved Human Amniotic Membrane." Current Eye Research, vol. 20, no. 3, 2000, pp. 173-7.
Koizumi NJ, Inatomi TJ, Sotozono CJ, et al. Growth factor mRNA and protein in preserved human amniotic membrane. Curr Eye Res. 2000;20(3):173-7.
Koizumi, N. J., Inatomi, T. J., Sotozono, C. J., Fullwood, N. J., Quantock, A. J., & Kinoshita, S. (2000). Growth factor mRNA and protein in preserved human amniotic membrane. Current Eye Research, 20(3), 173-7.
Koizumi NJ, et al. Growth Factor mRNA and Protein in Preserved Human Amniotic Membrane. Curr Eye Res. 2000;20(3):173-7. PubMed PMID: 10694891.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth factor mRNA and protein in preserved human amniotic membrane. AU - Koizumi,N J, AU - Inatomi,T J, AU - Sotozono,C J, AU - Fullwood,N J, AU - Quantock,A J, AU - Kinoshita,S, PY - 2000/3/1/pubmed PY - 2000/5/20/medline PY - 2000/3/1/entrez SP - 173 EP - 7 JF - Current eye research JO - Curr. Eye Res. VL - 20 IS - 3 N2 - PURPOSE: To investigate the expression of growth factor mRNA and the level of growth factor protein in preserved human amniotic membrane (AM). METHODS: RT-PCR was used to examine the expression of mRNA for eight growth factors (EGF, TGF-alpha, KGF, HGF, bFGF, TGF-beta1, -beta2, -beta3) and two growth factor receptors (KGFR and HGFR) in human AM preserved at -80 degrees C for one month. In addition, ELISAs were used to measure the protein concentrations of seven growth factors (EGF, TGF-alpha, KGF, HGF, bFGF, TGF-beta1, -beta2) in preserved human corneas and in AM both with and without amniotic epithelium. RESULTS: RT-PCR revealed that human AM expresses mRNA for EGF, TGF-alpha, KGF, HGF, bFGF, TGF-beta1, -beta2, -beta3, KGFR and HGFR, while ELISAs showed that it contains EGF, TGF-alpha, KGF, HGF, bFGF, TGF-beta1, -beta2. AM without amniotic epithelium also contains all seven growth factors examined, however, in this tissue the protein levels of EGF, KGF, HGF and bFGF were found to be significantly lower than in native AM. CONCLUSIONS: Preserved human AM expresses mRNAs for a number of growth factors and contains several growth factor proteins that might benefit epithelialization after AM transplantation. High levels of EGF, KGF, HGF and bFGF in AM with amniotic epithelium as compared to AM without amniotic epithelium suggest an epithelial origin for these growth factors. We feel that EGF, KGF and HGF in particular might play important roles in ocular surface wound healing after AM transplantation. SN - 0271-3683 UR - https://www.unboundmedicine.com/medline/citation/10694891/Growth_factor_mRNA_and_protein_in_preserved_human_amniotic_membrane_ L2 - https://www.lens.org/lens/search?q=citation_id:10694891 DB - PRIME DP - Unbound Medicine ER -