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Early aqueous humor analysis in patients with human ocular toxoplasmosis.
J Clin Microbiol 2000; 38(3):996-1001JC

Abstract

To evaluate the diagnostic sensitivity of a panel of laboratory tests for ocular toxoplasmosis performed at the time of presentation, paired samples of aqueous humor and serum were collected from 49 consecutive episodes of ocular toxoplasmosis with a clinical course of less than 3 weeks. Total immunoglobulin G (IgG) and Toxoplasma gondii-specific IgG, IgM, and IgA were quantified by enzyme-linked immunosorbent assay. The avidity of T. gondii-specific IgG was determined, and DNA extracted from aqueous humor was amplified for detection of a glycoprotein B gene sequence of T. gondii. The diagnosis was confirmed for 73% (36 of 49) of the patients; this rate rose to 79.5% if data from a later analysis of aqueous humor derived from five of the negative patients were included. The analysis of serum (detection of T. gondii-specific IgM and analysis of consecutive serum samples) alone did not contribute to the diagnosis. Calculation of local antibody production lacked diagnostic sensitivity when it was determined less than 3 weeks after the manifestation of clinical symptoms (28 of 49 patients [57%]), but this rose to 70% after an analysis of a second aqueous humor sample. The antibody avidity index attained diagnostic significance in only 8 of 43 instances (19%), and T. gondii DNA was amplified from no more than 6 of 39 (16%) aqueous humor samples. However, T. gondii-specific IgA was found within the aqueous humors of 11 of 43 patients (26%); measurement of the T. gondii-specific IgA level thus contributed substantially to the diagnostic sensitivity of the laboratory tests.

Authors+Show Affiliations

Department of Ophthalmology, University of Bern, Inselspital, CH-3010 Bern, Switzerland. justus.garweg@insel.chNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10698986

Citation

Garweg, J G., et al. "Early Aqueous Humor Analysis in Patients With Human Ocular Toxoplasmosis." Journal of Clinical Microbiology, vol. 38, no. 3, 2000, pp. 996-1001.
Garweg JG, Jacquier P, Boehnke M. Early aqueous humor analysis in patients with human ocular toxoplasmosis. J Clin Microbiol. 2000;38(3):996-1001.
Garweg, J. G., Jacquier, P., & Boehnke, M. (2000). Early aqueous humor analysis in patients with human ocular toxoplasmosis. Journal of Clinical Microbiology, 38(3), pp. 996-1001.
Garweg JG, Jacquier P, Boehnke M. Early Aqueous Humor Analysis in Patients With Human Ocular Toxoplasmosis. J Clin Microbiol. 2000;38(3):996-1001. PubMed PMID: 10698986.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Early aqueous humor analysis in patients with human ocular toxoplasmosis. AU - Garweg,J G, AU - Jacquier,P, AU - Boehnke,M, PY - 2000/3/4/pubmed PY - 2000/3/4/medline PY - 2000/3/4/entrez SP - 996 EP - 1001 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 38 IS - 3 N2 - To evaluate the diagnostic sensitivity of a panel of laboratory tests for ocular toxoplasmosis performed at the time of presentation, paired samples of aqueous humor and serum were collected from 49 consecutive episodes of ocular toxoplasmosis with a clinical course of less than 3 weeks. Total immunoglobulin G (IgG) and Toxoplasma gondii-specific IgG, IgM, and IgA were quantified by enzyme-linked immunosorbent assay. The avidity of T. gondii-specific IgG was determined, and DNA extracted from aqueous humor was amplified for detection of a glycoprotein B gene sequence of T. gondii. The diagnosis was confirmed for 73% (36 of 49) of the patients; this rate rose to 79.5% if data from a later analysis of aqueous humor derived from five of the negative patients were included. The analysis of serum (detection of T. gondii-specific IgM and analysis of consecutive serum samples) alone did not contribute to the diagnosis. Calculation of local antibody production lacked diagnostic sensitivity when it was determined less than 3 weeks after the manifestation of clinical symptoms (28 of 49 patients [57%]), but this rose to 70% after an analysis of a second aqueous humor sample. The antibody avidity index attained diagnostic significance in only 8 of 43 instances (19%), and T. gondii DNA was amplified from no more than 6 of 39 (16%) aqueous humor samples. However, T. gondii-specific IgA was found within the aqueous humors of 11 of 43 patients (26%); measurement of the T. gondii-specific IgA level thus contributed substantially to the diagnostic sensitivity of the laboratory tests. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/10698986/Early_aqueous_humor_analysis_in_patients_with_human_ocular_toxoplasmosis_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=10698986 DB - PRIME DP - Unbound Medicine ER -