Tags

Type your tag names separated by a space and hit enter

Ionizing radiation potentiates the induction of nitric oxide synthase by interferon-gamma (Ifn-gamma) or Ifn-gamma and lipopolysaccharide in bnl cl.2 murine embryonic liver cells: role of hydrogen peroxide.
Free Radic Biol Med 2000; 28(3):390-6FR

Abstract

The effects of ionizing irradiation on the nitric oxide (NO) production in murine embryonic liver cell line, BNL CL.2 cells, were investigated. Various doses (5-40 Gy) of radiation made BNL CL.2 cells responsive to interferon-gamma alone for the production of NO in a dose-dependent manner. Small amounts of lipopolysaccharide (LPS) or tumor necrosis factor-alpha (TNF-alpha) synergized with IFN-gamma in the production of NO from irradiated BNL CL.2 cells, even though LPS or TNF-alpha alone did not induce NO production from the same cells. Immunoblots showed parallel induction of inducible nitric oxide synthase (iNOS). NO production in irradiated BNL CL.2 cells by IFN-gamma or IFN-gamma plus LPS was decreased by the addition of catalase, suggesting that H(2)O(2) produced by ionizing irradiation primed the cells to trigger NO production in response to IFN-gamma or IFN-gamma plus LPS. Furthermore, the treatment of nongamma-irradiated BNL CL.2 cells with H(2)O(2) made the cells responsive to IFN-gamma or IFN-gamma plus LPS for the production of NO. This study shows that ionizing irradiation has the ability to induce iNOS gene expression in responsive to IFN-gamma via the formation of H(2)O(2) in BNL CL.2 murine embryonic liver cells.

Authors+Show Affiliations

Department of Microbiology and Immunology, Wonkwang University School of Medicine, and Medicinal Resource Research Center of Wonkwang University, Iksan, Chonbug, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10699750

Citation

Yoo, J C., et al. "Ionizing Radiation Potentiates the Induction of Nitric Oxide Synthase By Interferon-gamma (Ifn-gamma) or Ifn-gamma and Lipopolysaccharide in Bnl Cl.2 Murine Embryonic Liver Cells: Role of Hydrogen Peroxide." Free Radical Biology & Medicine, vol. 28, no. 3, 2000, pp. 390-6.
Yoo JC, Pae HO, Choi BM, et al. Ionizing radiation potentiates the induction of nitric oxide synthase by interferon-gamma (Ifn-gamma) or Ifn-gamma and lipopolysaccharide in bnl cl.2 murine embryonic liver cells: role of hydrogen peroxide. Free Radic Biol Med. 2000;28(3):390-6.
Yoo, J. C., Pae, H. O., Choi, B. M., Kim, W. I., Kim, J. D., Kim, Y. M., & Chung, H. T. (2000). Ionizing radiation potentiates the induction of nitric oxide synthase by interferon-gamma (Ifn-gamma) or Ifn-gamma and lipopolysaccharide in bnl cl.2 murine embryonic liver cells: role of hydrogen peroxide. Free Radical Biology & Medicine, 28(3), pp. 390-6.
Yoo JC, et al. Ionizing Radiation Potentiates the Induction of Nitric Oxide Synthase By Interferon-gamma (Ifn-gamma) or Ifn-gamma and Lipopolysaccharide in Bnl Cl.2 Murine Embryonic Liver Cells: Role of Hydrogen Peroxide. Free Radic Biol Med. 2000 Feb 1;28(3):390-6. PubMed PMID: 10699750.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ionizing radiation potentiates the induction of nitric oxide synthase by interferon-gamma (Ifn-gamma) or Ifn-gamma and lipopolysaccharide in bnl cl.2 murine embryonic liver cells: role of hydrogen peroxide. AU - Yoo,J C, AU - Pae,H O, AU - Choi,B M, AU - Kim,W I, AU - Kim,J D, AU - Kim,Y M, AU - Chung,H T, PY - 2000/3/4/pubmed PY - 2000/4/15/medline PY - 2000/3/4/entrez SP - 390 EP - 6 JF - Free radical biology & medicine JO - Free Radic. Biol. Med. VL - 28 IS - 3 N2 - The effects of ionizing irradiation on the nitric oxide (NO) production in murine embryonic liver cell line, BNL CL.2 cells, were investigated. Various doses (5-40 Gy) of radiation made BNL CL.2 cells responsive to interferon-gamma alone for the production of NO in a dose-dependent manner. Small amounts of lipopolysaccharide (LPS) or tumor necrosis factor-alpha (TNF-alpha) synergized with IFN-gamma in the production of NO from irradiated BNL CL.2 cells, even though LPS or TNF-alpha alone did not induce NO production from the same cells. Immunoblots showed parallel induction of inducible nitric oxide synthase (iNOS). NO production in irradiated BNL CL.2 cells by IFN-gamma or IFN-gamma plus LPS was decreased by the addition of catalase, suggesting that H(2)O(2) produced by ionizing irradiation primed the cells to trigger NO production in response to IFN-gamma or IFN-gamma plus LPS. Furthermore, the treatment of nongamma-irradiated BNL CL.2 cells with H(2)O(2) made the cells responsive to IFN-gamma or IFN-gamma plus LPS for the production of NO. This study shows that ionizing irradiation has the ability to induce iNOS gene expression in responsive to IFN-gamma via the formation of H(2)O(2) in BNL CL.2 murine embryonic liver cells. SN - 0891-5849 UR - https://www.unboundmedicine.com/medline/citation/10699750/Ionizing_radiation_potentiates_the_induction_of_nitric_oxide_synthase_by_interferon_gamma__Ifn_gamma__or_Ifn_gamma_and_lipopolysaccharide_in_bnl_cl_2_murine_embryonic_liver_cells:_role_of_hydrogen_peroxide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-5849(99)00252-X DB - PRIME DP - Unbound Medicine ER -