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Dynorphin B and spinal analgesia: induction of antinociception by the cannabinoids CP55,940, Delta(9)-THC and anandamide.
Brain Res. 2000 Feb 28; 857(1-2):337-42.BR

Abstract

The endogenous opioid dynorphin B was evaluated for its role in cannabinoid-induced antinociception. Previous work in our laboratory has shown that the synthetic, bicyclic cannabinoid, CP55,940, induces the release of dynorphin B whilst the naturally occurring cannabinoid, Delta(9)-tetrahydrocannabinol (Delta(9)-THC), releases dynorphin A. The dynorphins contribute in part to the antinociceptive effects of both cannabinoids at the level of the spinal cord. The present study compares dynorphin B released from perfused rat spinal cord in response to acute administration of anandamide (AEA), Delta(9)-THC and CP55,940 at two time points, 10 min and 30 min post administration, and attempts to correlate such release with antinociceptive effects of the drugs. Dynorphin B was collected from spinal perfusates of rats pretreated with Delta(9)-THC, CP55,940 or AEA. The supernatant was lyophilized and the concentrations of dynorphin B were measured via radioimmunoassay. At a peak time of antinociception (10 min), CP55,940 and Delta(9)-THC induced significant two-fold increases in the release of dynorphin B. AEA did not significantly release dynorphin B. Upon a 30-min pretreatment with the drugs, no significant dynorphin B release was observed, although antinociceptive effects persisted for CP55,940 and Delta(9)-THC. Previous work indicates that Delta(9)-THC releases dynorphin A while AEA releases no dynorphin A. This study confirms that although all three test drugs produced significant antinociception at 10 min, the endocannabinoid, AEA, does not induce antinociception via dynorphin release. Thus, our data indicate a distinct mechanism which underlies AEA-induced antinociception.

Authors+Show Affiliations

Department of Nurse Anesthesia, Virginia Commonwealth University, Richmond, VA 23298-0613, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10700588

Citation

Houser, S J., et al. "Dynorphin B and Spinal Analgesia: Induction of Antinociception By the Cannabinoids CP55,940, Delta(9)-THC and Anandamide." Brain Research, vol. 857, no. 1-2, 2000, pp. 337-42.
Houser SJ, Eads M, Embrey JP, et al. Dynorphin B and spinal analgesia: induction of antinociception by the cannabinoids CP55,940, Delta(9)-THC and anandamide. Brain Res. 2000;857(1-2):337-42.
Houser, S. J., Eads, M., Embrey, J. P., & Welch, S. P. (2000). Dynorphin B and spinal analgesia: induction of antinociception by the cannabinoids CP55,940, Delta(9)-THC and anandamide. Brain Research, 857(1-2), 337-42.
Houser SJ, et al. Dynorphin B and Spinal Analgesia: Induction of Antinociception By the Cannabinoids CP55,940, Delta(9)-THC and Anandamide. Brain Res. 2000 Feb 28;857(1-2):337-42. PubMed PMID: 10700588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynorphin B and spinal analgesia: induction of antinociception by the cannabinoids CP55,940, Delta(9)-THC and anandamide. AU - Houser,S J, AU - Eads,M, AU - Embrey,J P, AU - Welch,S P, PY - 2000/3/4/pubmed PY - 2000/5/8/medline PY - 2000/3/4/entrez SP - 337 EP - 42 JF - Brain research JO - Brain Res VL - 857 IS - 1-2 N2 - The endogenous opioid dynorphin B was evaluated for its role in cannabinoid-induced antinociception. Previous work in our laboratory has shown that the synthetic, bicyclic cannabinoid, CP55,940, induces the release of dynorphin B whilst the naturally occurring cannabinoid, Delta(9)-tetrahydrocannabinol (Delta(9)-THC), releases dynorphin A. The dynorphins contribute in part to the antinociceptive effects of both cannabinoids at the level of the spinal cord. The present study compares dynorphin B released from perfused rat spinal cord in response to acute administration of anandamide (AEA), Delta(9)-THC and CP55,940 at two time points, 10 min and 30 min post administration, and attempts to correlate such release with antinociceptive effects of the drugs. Dynorphin B was collected from spinal perfusates of rats pretreated with Delta(9)-THC, CP55,940 or AEA. The supernatant was lyophilized and the concentrations of dynorphin B were measured via radioimmunoassay. At a peak time of antinociception (10 min), CP55,940 and Delta(9)-THC induced significant two-fold increases in the release of dynorphin B. AEA did not significantly release dynorphin B. Upon a 30-min pretreatment with the drugs, no significant dynorphin B release was observed, although antinociceptive effects persisted for CP55,940 and Delta(9)-THC. Previous work indicates that Delta(9)-THC releases dynorphin A while AEA releases no dynorphin A. This study confirms that although all three test drugs produced significant antinociception at 10 min, the endocannabinoid, AEA, does not induce antinociception via dynorphin release. Thus, our data indicate a distinct mechanism which underlies AEA-induced antinociception. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/10700588/Dynorphin_B_and_spinal_analgesia:_induction_of_antinociception_by_the_cannabinoids_CP55940_Delta_9__THC_and_anandamide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(00)01981-8 DB - PRIME DP - Unbound Medicine ER -