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Sciatic nerve of normal and T200x5R Swiss white mice fails to support multiplication of intraneurally injected M. leprae.
Int J Lepr Other Mycobact Dis. 1999 Dec; 67(4):446-52.IJ

Abstract

In a preliminary study we have shown that freshly harvested Mycobacterium leprae, when injected into the sciatic nerve in normal and immunosuppressed (TR) mice, induce massive but localized epithelioid and macrophage granuloma, respectively, in 3-4 weeks. In order to determine the fate of M. leprae injected intraneurally into normal and TR mice, in the present study we measured sequentially the viability, fold increase and clearance, if any, using semi-quantitative methods. The average M. leprae yield per nerve assessed at regular intervals, beginning at 24 hr and including 72 hr, 1 week, 2, 3, 4, 12, 24 and 48 weeks, showed neither a significant increase nor a decrease in either the normal or the TR mice. The viability of M. leprae, assessed using the standard mouse foot pad method, showed a significant decrease as compared to baseline growth effective at 24 hr and remained static until approximately 4 weeks. A further decline and total loss of viability was noted by 12 months. The results show that injection of M. leprae via the intraneural route in both normal and TR mice failed to sustain the viability and failed to support the multiplication of the organisms.

Authors+Show Affiliations

Foundation for Medical Research, Thadani Marg, Worli, Mumbai, India. frchbom@bom2.vsnl.net.inNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10700920

Citation

Shetty, V P., et al. "Sciatic Nerve of Normal and T200x5R Swiss White Mice Fails to Support Multiplication of Intraneurally Injected M. Leprae." International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association, vol. 67, no. 4, 1999, pp. 446-52.
Shetty VP, Matharu PS, Antia NH. Sciatic nerve of normal and T200x5R Swiss white mice fails to support multiplication of intraneurally injected M. leprae. Int J Lepr Other Mycobact Dis. 1999;67(4):446-52.
Shetty, V. P., Matharu, P. S., & Antia, N. H. (1999). Sciatic nerve of normal and T200x5R Swiss white mice fails to support multiplication of intraneurally injected M. leprae. International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association, 67(4), 446-52.
Shetty VP, Matharu PS, Antia NH. Sciatic Nerve of Normal and T200x5R Swiss White Mice Fails to Support Multiplication of Intraneurally Injected M. Leprae. Int J Lepr Other Mycobact Dis. 1999;67(4):446-52. PubMed PMID: 10700920.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sciatic nerve of normal and T200x5R Swiss white mice fails to support multiplication of intraneurally injected M. leprae. AU - Shetty,V P, AU - Matharu,P S, AU - Antia,N H, PY - 2000/3/4/pubmed PY - 2000/3/25/medline PY - 2000/3/4/entrez SP - 446 EP - 52 JF - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association JO - Int J Lepr Other Mycobact Dis VL - 67 IS - 4 N2 - In a preliminary study we have shown that freshly harvested Mycobacterium leprae, when injected into the sciatic nerve in normal and immunosuppressed (TR) mice, induce massive but localized epithelioid and macrophage granuloma, respectively, in 3-4 weeks. In order to determine the fate of M. leprae injected intraneurally into normal and TR mice, in the present study we measured sequentially the viability, fold increase and clearance, if any, using semi-quantitative methods. The average M. leprae yield per nerve assessed at regular intervals, beginning at 24 hr and including 72 hr, 1 week, 2, 3, 4, 12, 24 and 48 weeks, showed neither a significant increase nor a decrease in either the normal or the TR mice. The viability of M. leprae, assessed using the standard mouse foot pad method, showed a significant decrease as compared to baseline growth effective at 24 hr and remained static until approximately 4 weeks. A further decline and total loss of viability was noted by 12 months. The results show that injection of M. leprae via the intraneural route in both normal and TR mice failed to sustain the viability and failed to support the multiplication of the organisms. SN - 0148-916X UR - https://www.unboundmedicine.com/medline/citation/10700920/Sciatic_nerve_of_normal_and_T200x5R_Swiss_white_mice_fails_to_support_multiplication_of_intraneurally_injected_M__leprae_ DB - PRIME DP - Unbound Medicine ER -