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Relationship between neurokinin-1 receptor and substance P in the striatum: light and electron microscopic immunohistochemical study in the rat.
J Comp Neurol. 2000 Mar 06; 418(2):156-63.JC

Abstract

The synaptic relationship between substance P (SP) and its receptor, i.e., neurokinin-1 receptor (NK1R), was examined in the striatum of the rat by confocal laser-scanning microscopy and electron microscopy. For confocal laser-scanning microscopy, triple-immunofluorescence histochemistry was performed to label NK1R, SP, and vesicular acetylcholine transporter (a specific marker for cholinergic neurons). In electron microscopic double-immunolabeling study, immunoreactivity for NK1R was detected with the silver-intensified gold method, while immunoreactivity for SP was detected with peroxidase immunohistochemistry. Simultaneous immunolabeling of NK1R and SP revealed significant mismatch at the synaptic level: although some SP-immunopositive axon terminals were in synaptic contact with NK1R-immunopositive sites of plasma membrane, NK1R-immunoreactivity was observed at both synaptic and non-synaptic sites of plasma membrane. Thus, SP released from the sites remote from NK1Rs might diffuse in the extracellular fluid to act, as a paracrine neurotransmitter, on NK1Rs distant from its releasing site. SP neurotransmission in the striatum might occur not only synaptically but also extrasynaptically. The SP-NK1R system might constitute an association system within the striatum.

Authors+Show Affiliations

Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10701441

Citation

Li, J L., et al. "Relationship Between Neurokinin-1 Receptor and Substance P in the Striatum: Light and Electron Microscopic Immunohistochemical Study in the Rat." The Journal of Comparative Neurology, vol. 418, no. 2, 2000, pp. 156-63.
Li JL, Wang D, Kaneko T, et al. Relationship between neurokinin-1 receptor and substance P in the striatum: light and electron microscopic immunohistochemical study in the rat. J Comp Neurol. 2000;418(2):156-63.
Li, J. L., Wang, D., Kaneko, T., Shigemoto, R., Nomura, S., & Mizuno, N. (2000). Relationship between neurokinin-1 receptor and substance P in the striatum: light and electron microscopic immunohistochemical study in the rat. The Journal of Comparative Neurology, 418(2), 156-63.
Li JL, et al. Relationship Between Neurokinin-1 Receptor and Substance P in the Striatum: Light and Electron Microscopic Immunohistochemical Study in the Rat. J Comp Neurol. 2000 Mar 6;418(2):156-63. PubMed PMID: 10701441.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relationship between neurokinin-1 receptor and substance P in the striatum: light and electron microscopic immunohistochemical study in the rat. AU - Li,J L, AU - Wang,D, AU - Kaneko,T, AU - Shigemoto,R, AU - Nomura,S, AU - Mizuno,N, PY - 2000/3/4/pubmed PY - 2000/3/18/medline PY - 2000/3/4/entrez SP - 156 EP - 63 JF - The Journal of comparative neurology JO - J Comp Neurol VL - 418 IS - 2 N2 - The synaptic relationship between substance P (SP) and its receptor, i.e., neurokinin-1 receptor (NK1R), was examined in the striatum of the rat by confocal laser-scanning microscopy and electron microscopy. For confocal laser-scanning microscopy, triple-immunofluorescence histochemistry was performed to label NK1R, SP, and vesicular acetylcholine transporter (a specific marker for cholinergic neurons). In electron microscopic double-immunolabeling study, immunoreactivity for NK1R was detected with the silver-intensified gold method, while immunoreactivity for SP was detected with peroxidase immunohistochemistry. Simultaneous immunolabeling of NK1R and SP revealed significant mismatch at the synaptic level: although some SP-immunopositive axon terminals were in synaptic contact with NK1R-immunopositive sites of plasma membrane, NK1R-immunoreactivity was observed at both synaptic and non-synaptic sites of plasma membrane. Thus, SP released from the sites remote from NK1Rs might diffuse in the extracellular fluid to act, as a paracrine neurotransmitter, on NK1Rs distant from its releasing site. SP neurotransmission in the striatum might occur not only synaptically but also extrasynaptically. The SP-NK1R system might constitute an association system within the striatum. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/10701441/Relationship_between_neurokinin_1_receptor_and_substance_P_in_the_striatum:_light_and_electron_microscopic_immunohistochemical_study_in_the_rat_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=2000&volume=418&issue=2&spage=156 DB - PRIME DP - Unbound Medicine ER -