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Galectin-3 induces endothelial cell morphogenesis and angiogenesis.
Am J Pathol. 2000 Mar; 156(3):899-909.AJ

Abstract

Increasing evidence suggests that carbohydrate-binding proteins play an essential role in tumor growth and metastasis. However, conflicting results on their function in the regulation of cell proliferation and differentiation during angiogenesis have been reported. We have examined the role of galectin-3 in the regulation of human umbilical vein endothelial cell proliferation, differentiation, migration, and neovascularization. Galectin-3, a carbohydrate-binding protein, with specificity for type 1 and 11 ABH blood group epitopes and polylactosamine glycan containing cell surface glycoproteins, is the major nonintegrin cellular laminin-binding protein. Because galectin-3 expression was shown to be associated in some tumor systems with metastasis, we questioned whether it induces endothelial cell morphogenesis. Here we show that galectin-3 affects chemotaxis and morphology and stimulates capillary tube formation of HUV-EC-C in vitro and angiogenesis in vivo. Endothelial cell morphogenesis is a carbohydrate-dependent process, as it is neutralized by specific sugars and antibodies. These findings demonstrate that endothelial cell surface carbohydrate recognition event(s) can induce a signaling cascade leading to the differentiation and angiogenesis of endothelial cells.

Authors+Show Affiliations

Department of Tumor Progression, Karmanos Cancer Institute, Detroit, Michigan 48201, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10702407

Citation

Nangia-Makker, P, et al. "Galectin-3 Induces Endothelial Cell Morphogenesis and Angiogenesis." The American Journal of Pathology, vol. 156, no. 3, 2000, pp. 899-909.
Nangia-Makker P, Honjo Y, Sarvis R, et al. Galectin-3 induces endothelial cell morphogenesis and angiogenesis. Am J Pathol. 2000;156(3):899-909.
Nangia-Makker, P., Honjo, Y., Sarvis, R., Akahani, S., Hogan, V., Pienta, K. J., & Raz, A. (2000). Galectin-3 induces endothelial cell morphogenesis and angiogenesis. The American Journal of Pathology, 156(3), 899-909.
Nangia-Makker P, et al. Galectin-3 Induces Endothelial Cell Morphogenesis and Angiogenesis. Am J Pathol. 2000;156(3):899-909. PubMed PMID: 10702407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Galectin-3 induces endothelial cell morphogenesis and angiogenesis. AU - Nangia-Makker,P, AU - Honjo,Y, AU - Sarvis,R, AU - Akahani,S, AU - Hogan,V, AU - Pienta,K J, AU - Raz,A, PY - 2000/3/7/pubmed PY - 2000/3/25/medline PY - 2000/3/7/entrez SP - 899 EP - 909 JF - The American journal of pathology JO - Am J Pathol VL - 156 IS - 3 N2 - Increasing evidence suggests that carbohydrate-binding proteins play an essential role in tumor growth and metastasis. However, conflicting results on their function in the regulation of cell proliferation and differentiation during angiogenesis have been reported. We have examined the role of galectin-3 in the regulation of human umbilical vein endothelial cell proliferation, differentiation, migration, and neovascularization. Galectin-3, a carbohydrate-binding protein, with specificity for type 1 and 11 ABH blood group epitopes and polylactosamine glycan containing cell surface glycoproteins, is the major nonintegrin cellular laminin-binding protein. Because galectin-3 expression was shown to be associated in some tumor systems with metastasis, we questioned whether it induces endothelial cell morphogenesis. Here we show that galectin-3 affects chemotaxis and morphology and stimulates capillary tube formation of HUV-EC-C in vitro and angiogenesis in vivo. Endothelial cell morphogenesis is a carbohydrate-dependent process, as it is neutralized by specific sugars and antibodies. These findings demonstrate that endothelial cell surface carbohydrate recognition event(s) can induce a signaling cascade leading to the differentiation and angiogenesis of endothelial cells. SN - 0002-9440 UR - https://www.unboundmedicine.com/medline/citation/10702407/Galectin_3_induces_endothelial_cell_morphogenesis_and_angiogenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0002-9440(10)64959-0 DB - PRIME DP - Unbound Medicine ER -