Tags

Type your tag names separated by a space and hit enter

Subcutaneous adipose tissue expression of plasminogen activator inhibitor-1 gene during very low calorie diet in obese subjects.

Abstract

OBJECTIVE

To determine whether changes in subcutaneous adipose tissue plasminogen activator inhibitor-1 (PAI-1) expression influence plasma PAI-1 level during weight loss in obese humans.

DESIGN

Study of the variations of PAI-1 levels both in plasma and in subcutaneous abdominal adipose tissue in 15 volunteer non-diabetic obese subjects, body mass index (BMI) 40.4.+/-1.9 kg/m2, aged 48+/-3 y, before and after a 3 week very low calorie diet (VLCD) programme (3.9+/-0.1 MJ/day).

MEASUREMENTS

Plasma and adipose tissue PAI-1 protein levels were measured by enzyme-linked immunosorbent assay and PAI-1 mRNA levels were quantified by quantitative RT-competitive PCR.

RESULTS

VLCD induced weight loss (5.8+/-0.8 kg) and decreased plasma PAI-1 concentration (-26% (P<0. 01)). Surprisingly, PAI-1 mRNA and protein abundance in subcutaneous adipose tissue increased by 87% (P<0.05) and by 44% (P<0.01), respectively.

CONCLUSION

These data indicate thus that changes in subcutaneous adipose tissue PAI-1 expression are not involved in the decrease of plasma PAI-1 levels during VLCD in obese subjects. International Journal of Obesity (2000)24, 70-74

Authors+Show Affiliations

Service de Biochimie, Hôpital de la Salpêtrière, AP-HP, Paris. jean-philippe.bastard@psl.ap-hop-paris.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10702753

Citation

Bastard, J P., et al. "Subcutaneous Adipose Tissue Expression of Plasminogen Activator Inhibitor-1 Gene During Very Low Calorie Diet in Obese Subjects." International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity, vol. 24, no. 1, 2000, pp. 70-4.
Bastard JP, Vidal H, Jardel C, et al. Subcutaneous adipose tissue expression of plasminogen activator inhibitor-1 gene during very low calorie diet in obese subjects. Int J Obes Relat Metab Disord. 2000;24(1):70-4.
Bastard, J. P., Vidal, H., Jardel, C., Bruckert, E., Robin, D., Vallier, P., ... Hainque, B. (2000). Subcutaneous adipose tissue expression of plasminogen activator inhibitor-1 gene during very low calorie diet in obese subjects. International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity, 24(1), pp. 70-4.
Bastard JP, et al. Subcutaneous Adipose Tissue Expression of Plasminogen Activator Inhibitor-1 Gene During Very Low Calorie Diet in Obese Subjects. Int J Obes Relat Metab Disord. 2000;24(1):70-4. PubMed PMID: 10702753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Subcutaneous adipose tissue expression of plasminogen activator inhibitor-1 gene during very low calorie diet in obese subjects. AU - Bastard,J P, AU - Vidal,H, AU - Jardel,C, AU - Bruckert,E, AU - Robin,D, AU - Vallier,P, AU - Blondy,P, AU - Turpin,G, AU - Forest,C, AU - Hainque,B, PY - 2000/3/7/pubmed PY - 2000/3/7/medline PY - 2000/3/7/entrez SP - 70 EP - 4 JF - International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity JO - Int. J. Obes. Relat. Metab. Disord. VL - 24 IS - 1 N2 - OBJECTIVE: To determine whether changes in subcutaneous adipose tissue plasminogen activator inhibitor-1 (PAI-1) expression influence plasma PAI-1 level during weight loss in obese humans. DESIGN: Study of the variations of PAI-1 levels both in plasma and in subcutaneous abdominal adipose tissue in 15 volunteer non-diabetic obese subjects, body mass index (BMI) 40.4.+/-1.9 kg/m2, aged 48+/-3 y, before and after a 3 week very low calorie diet (VLCD) programme (3.9+/-0.1 MJ/day). MEASUREMENTS: Plasma and adipose tissue PAI-1 protein levels were measured by enzyme-linked immunosorbent assay and PAI-1 mRNA levels were quantified by quantitative RT-competitive PCR. RESULTS: VLCD induced weight loss (5.8+/-0.8 kg) and decreased plasma PAI-1 concentration (-26% (P<0. 01)). Surprisingly, PAI-1 mRNA and protein abundance in subcutaneous adipose tissue increased by 87% (P<0.05) and by 44% (P<0.01), respectively. CONCLUSION: These data indicate thus that changes in subcutaneous adipose tissue PAI-1 expression are not involved in the decrease of plasma PAI-1 levels during VLCD in obese subjects. International Journal of Obesity (2000)24, 70-74 UR - https://www.unboundmedicine.com/medline/citation/10702753/Subcutaneous_adipose_tissue_expression_of_plasminogen_activator_inhibitor_1_gene_during_very_low_calorie_diet_in_obese_subjects_ L2 - https://medlineplus.gov/obesity.html DB - PRIME DP - Unbound Medicine ER -