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Lack of expression of surface immunoglobulin light chains in B-cell non-Hodgkin lymphomas.
Am J Clin Pathol. 2000 Mar; 113(3):399-405.AJ

Abstract

We describe 10 cases of B-cell non-Hodgkin lymphoma (NHL) that did not express immunoglobulin kappa or lambda light chains by dual-color flow cytometry. Cases were identified from 298 consecutive cases of B-cell NHL and included follicular center cell lymphoma, diffuse large B-cell lymphoma, small noncleaved cell lymphoma, and small lymphocytic lymphoma. One case did not express any immunoglobulin heavy chain (IgH) as well; however, isolated expression of IgG heavy chain was seen in another case. Immunoglobulin heavy chains were not part of the lymphoma panel in other cases. All 3 cases in which gene rearrangement studies were performed showed rearrangement of IgH genes, including the case that did not express surface IgH chains. Immunoglobulin kappa light chain genes were rearranged in 2 of 3 cases and were in germline configuration in the third. All 147 cases of benign lymph nodes analyzed by flow cytometry showed polyclonal expression of immunoglobulin kappa and lambda light chains. Because of the absence of surface immunoglobulin light chains, these tumors must be distinguished from precursor B-cell acute lymphoblastic leukemia, plasma cell tumors, and rare cases of florid follicular hyperplasia that do not express surface immunoglobulins. The absence of immunoglobulin expression on malignant B cells can result from defects at any level from gene transcription to translocation of fully assembled proteins to the cell surface.

Authors+Show Affiliations

Lauren V. Ackerman Laboratory of Surgical Pathology, Washington University Medical Center, St Louis, MO 63110, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10705821

Citation

Kaleem, Z, et al. "Lack of Expression of Surface Immunoglobulin Light Chains in B-cell non-Hodgkin Lymphomas." American Journal of Clinical Pathology, vol. 113, no. 3, 2000, pp. 399-405.
Kaleem Z, Zehnbauer BA, White G, et al. Lack of expression of surface immunoglobulin light chains in B-cell non-Hodgkin lymphomas. Am J Clin Pathol. 2000;113(3):399-405.
Kaleem, Z., Zehnbauer, B. A., White, G., & Zutter, M. M. (2000). Lack of expression of surface immunoglobulin light chains in B-cell non-Hodgkin lymphomas. American Journal of Clinical Pathology, 113(3), 399-405.
Kaleem Z, et al. Lack of Expression of Surface Immunoglobulin Light Chains in B-cell non-Hodgkin Lymphomas. Am J Clin Pathol. 2000;113(3):399-405. PubMed PMID: 10705821.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lack of expression of surface immunoglobulin light chains in B-cell non-Hodgkin lymphomas. AU - Kaleem,Z, AU - Zehnbauer,B A, AU - White,G, AU - Zutter,M M, PY - 2000/3/8/pubmed PY - 2000/3/25/medline PY - 2000/3/8/entrez SP - 399 EP - 405 JF - American journal of clinical pathology JO - Am J Clin Pathol VL - 113 IS - 3 N2 - We describe 10 cases of B-cell non-Hodgkin lymphoma (NHL) that did not express immunoglobulin kappa or lambda light chains by dual-color flow cytometry. Cases were identified from 298 consecutive cases of B-cell NHL and included follicular center cell lymphoma, diffuse large B-cell lymphoma, small noncleaved cell lymphoma, and small lymphocytic lymphoma. One case did not express any immunoglobulin heavy chain (IgH) as well; however, isolated expression of IgG heavy chain was seen in another case. Immunoglobulin heavy chains were not part of the lymphoma panel in other cases. All 3 cases in which gene rearrangement studies were performed showed rearrangement of IgH genes, including the case that did not express surface IgH chains. Immunoglobulin kappa light chain genes were rearranged in 2 of 3 cases and were in germline configuration in the third. All 147 cases of benign lymph nodes analyzed by flow cytometry showed polyclonal expression of immunoglobulin kappa and lambda light chains. Because of the absence of surface immunoglobulin light chains, these tumors must be distinguished from precursor B-cell acute lymphoblastic leukemia, plasma cell tumors, and rare cases of florid follicular hyperplasia that do not express surface immunoglobulins. The absence of immunoglobulin expression on malignant B cells can result from defects at any level from gene transcription to translocation of fully assembled proteins to the cell surface. SN - 0002-9173 UR - https://www.unboundmedicine.com/medline/citation/10705821/Lack_of_expression_of_surface_immunoglobulin_light_chains_in_B_cell_non_Hodgkin_lymphomas_ L2 - https://academic.oup.com/ajcp/article-lookup/doi/10.1309/28ED-MM0T-DT3B-MT4P DB - PRIME DP - Unbound Medicine ER -