Tags

Type your tag names separated by a space and hit enter

Three conserved members of the RNase D family have unique and overlapping functions in the processing of 5S, 5.8S, U4, U5, RNase MRP and RNase P RNAs in yeast.
EMBO J. 2000 Mar 15; 19(6):1357-65.EJ

Abstract

The biogenesis of a number of RNA species in eukaryotic cells requires 3' processing. To determine the enzymes responsible for these trimming events, we created yeast strains lacking specific 3' to 5' exonucleases. In this work, we describe the analysis of three members of the RNase D family of exonucleases (Rex1p, Rex2p and Rex3p). This work led to three important conclusions. First, each of these exonucleases is required for the processing of distinct RNAs. Specifically, Rex1p, Rex2p and Rex3p are required for 5S rRNA, U4 snRNA and MRP RNA trimming, respectively. Secondly, some 3' exonucleases are redundant with other exonucleases. Specifically, Rex1p and Rex2p function redundantly in 5.8S rRNA maturation, Rex1p, Rex2p and Rex3p are redundant for the processing of U5 snRNA and RNase P RNA, and Rex1p and the exonuclease Rrp6p have an unknown redundant essential function. Thirdly, the demonstration that the Rex proteins can affect reactions that have been attributed previously to the exosome complex indicates that an apparently simple processing step can be surprisingly complex with multiple exonucleases working sequentially in the same pathway.

Authors+Show Affiliations

Department of Molecular Biology, Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA. ambro@u.arizona.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

10716935

Citation

van Hoof, A, et al. "Three Conserved Members of the RNase D Family Have Unique and Overlapping Functions in the Processing of 5S, 5.8S, U4, U5, RNase MRP and RNase P RNAs in Yeast." The EMBO Journal, vol. 19, no. 6, 2000, pp. 1357-65.
van Hoof A, Lennertz P, Parker R. Three conserved members of the RNase D family have unique and overlapping functions in the processing of 5S, 5.8S, U4, U5, RNase MRP and RNase P RNAs in yeast. EMBO J. 2000;19(6):1357-65.
van Hoof, A., Lennertz, P., & Parker, R. (2000). Three conserved members of the RNase D family have unique and overlapping functions in the processing of 5S, 5.8S, U4, U5, RNase MRP and RNase P RNAs in yeast. The EMBO Journal, 19(6), 1357-65.
van Hoof A, Lennertz P, Parker R. Three Conserved Members of the RNase D Family Have Unique and Overlapping Functions in the Processing of 5S, 5.8S, U4, U5, RNase MRP and RNase P RNAs in Yeast. EMBO J. 2000 Mar 15;19(6):1357-65. PubMed PMID: 10716935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Three conserved members of the RNase D family have unique and overlapping functions in the processing of 5S, 5.8S, U4, U5, RNase MRP and RNase P RNAs in yeast. AU - van Hoof,A, AU - Lennertz,P, AU - Parker,R, PY - 2000/3/16/pubmed PY - 2000/4/29/medline PY - 2000/3/16/entrez SP - 1357 EP - 65 JF - The EMBO journal JO - EMBO J VL - 19 IS - 6 N2 - The biogenesis of a number of RNA species in eukaryotic cells requires 3' processing. To determine the enzymes responsible for these trimming events, we created yeast strains lacking specific 3' to 5' exonucleases. In this work, we describe the analysis of three members of the RNase D family of exonucleases (Rex1p, Rex2p and Rex3p). This work led to three important conclusions. First, each of these exonucleases is required for the processing of distinct RNAs. Specifically, Rex1p, Rex2p and Rex3p are required for 5S rRNA, U4 snRNA and MRP RNA trimming, respectively. Secondly, some 3' exonucleases are redundant with other exonucleases. Specifically, Rex1p and Rex2p function redundantly in 5.8S rRNA maturation, Rex1p, Rex2p and Rex3p are redundant for the processing of U5 snRNA and RNase P RNA, and Rex1p and the exonuclease Rrp6p have an unknown redundant essential function. Thirdly, the demonstration that the Rex proteins can affect reactions that have been attributed previously to the exosome complex indicates that an apparently simple processing step can be surprisingly complex with multiple exonucleases working sequentially in the same pathway. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/10716935/Three_conserved_members_of_the_RNase_D_family_have_unique_and_overlapping_functions_in_the_processing_of_5S_5_8S_U4_U5_RNase_MRP_and_RNase_P_RNAs_in_yeast_ L2 - https://doi.org/10.1093/emboj/19.6.1357 DB - PRIME DP - Unbound Medicine ER -