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Automated liquid membrane extraction for high-performance liquid chromatography of Ropivacaine metabolites in urine.
J Chromatogr A. 2000 Feb 18; 870(1-2):151-7.JC

Abstract

An automatic method for the determination of metabolites of Ropivacaine in urine was set up. It utilizes supported liquid membrane extraction for sample clean-up and enrichment, followed by ion-pair chromatography determination using UV detection. The extraction was very selective with no observed interfering compounds from the urine matrix, permitting simple isocratic chromatographic analysis. The detection limits for spiked urine samples were 2-18 nM for the different compounds. The repeatability was 1-3% (RSD) with an internal standard that was also extracted, and about twice without this standard. A throughput of 3.3 samples per hour was achieved and the liquid membrane was stable for more than a week.

Authors+Show Affiliations

Department of Analytical Chemistry, Lund University, Sweden. jan_ake.jonsson@analykem.lu.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10722072

Citation

Jönsson, J A., et al. "Automated Liquid Membrane Extraction for High-performance Liquid Chromatography of Ropivacaine Metabolites in Urine." Journal of Chromatography. A, vol. 870, no. 1-2, 2000, pp. 151-7.
Jönsson JA, Andersson M, Melander C, et al. Automated liquid membrane extraction for high-performance liquid chromatography of Ropivacaine metabolites in urine. J Chromatogr A. 2000;870(1-2):151-7.
Jönsson, J. A., Andersson, M., Melander, C., Norberg, J., Thordarson, E., & Mathiasson, L. (2000). Automated liquid membrane extraction for high-performance liquid chromatography of Ropivacaine metabolites in urine. Journal of Chromatography. A, 870(1-2), 151-7.
Jönsson JA, et al. Automated Liquid Membrane Extraction for High-performance Liquid Chromatography of Ropivacaine Metabolites in Urine. J Chromatogr A. 2000 Feb 18;870(1-2):151-7. PubMed PMID: 10722072.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Automated liquid membrane extraction for high-performance liquid chromatography of Ropivacaine metabolites in urine. AU - Jönsson,J A, AU - Andersson,M, AU - Melander,C, AU - Norberg,J, AU - Thordarson,E, AU - Mathiasson,L, PY - 2000/3/18/pubmed PY - 2000/4/25/medline PY - 2000/3/18/entrez SP - 151 EP - 7 JF - Journal of chromatography. A JO - J Chromatogr A VL - 870 IS - 1-2 N2 - An automatic method for the determination of metabolites of Ropivacaine in urine was set up. It utilizes supported liquid membrane extraction for sample clean-up and enrichment, followed by ion-pair chromatography determination using UV detection. The extraction was very selective with no observed interfering compounds from the urine matrix, permitting simple isocratic chromatographic analysis. The detection limits for spiked urine samples were 2-18 nM for the different compounds. The repeatability was 1-3% (RSD) with an internal standard that was also extracted, and about twice without this standard. A throughput of 3.3 samples per hour was achieved and the liquid membrane was stable for more than a week. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/10722072/Automated_liquid_membrane_extraction_for_high_performance_liquid_chromatography_of_Ropivacaine_metabolites_in_urine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(99)00971-1 DB - PRIME DP - Unbound Medicine ER -