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Markers of intestinally-derived lipoproteins: application to studies of altered diet and meal fatty acid compositions.

Abstract

BACKGROUND AND AIM

The atherogenic potential of dietary derived lipids, chylomicrons (CM) and their remnants (CMr) is now becoming more widely recognised. To investigate factors effecting levels of CM and CMr and their importance in coronary heart disease risk it is essential to use a specific method of quantification. Two studies were carried out to investigate: (i) effects of increased daily intake of long chain n-3 polyunsaturated fatty acid (LC n-3 PUFA), and (ii) effects of increasing meal monounsaturated fatty acid (MUFA) content on the postprandial response of intestinally-derived lipoproteins. The contribution of the intestinally-derived lipoproteins to total lipaemia was assessed by triacylglycerol-rich lipoprotein (TRL) apolipoprotein B-48 (apo B-48) and retinyl ester (RE) concentrations.

METHODS AND RESULTS

In a randomised controlled crossover trial (placebo vs LC n-3 PUFA) a mean daily intake of 1.4 g/day of LC n-3 PUFA failed to reduce fasting and postprandial triacylglycerol (TAG) response in 9 healthy male volunteers. Although the pattern and nature of the apo B-48 response was consistent with the TAG response following the two diets, the postprandial RE response differed on the LC n-3 PUFA diet with a lower early RE response and a delayed and more marked increase in RE in the late postprandial period compared with the control diet, but the differences did not reach levels of statistical significance. In the meal study there was no effect of MUFA/SFA content on the total lipaemic response to the meals nor on the contribution of intestinally derived lipoproteins evaluated as TAG, apo B-48 and RE responses in the TRL fraction. In both studies, the RE and apo B-48 measurements provided broadly similar information with respect to lack of effects of dietary or meal fatty acid composition and the presence of single or multiple peak responses. However the apo B-48 and RE measurements differed with respect to the timing of their peak response times, with a delayed RE peak, relalive to apo B-48, of approximately 2-3 hours for the LC n-3 PUFA diet (p = 0.002) study and 1-1.5 hours for the meal MUFA/SFA study.

CONCLUSIONS

It was concluded that there are limitations of using RE as a specific CM marker, apo B-48 quantitation was found to be a more appropriate method for CM and CMr quantitation. However it was still considered of value to measure RE as it provided additional information regarding the incorporation of other constituents into the CM particle.

Authors+Show Affiliations

,

Hugh Sinclair Unit of Human Nutrition, Department of Food Science and Technology, University of Reading, Whiteknights, UK.

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Source

MeSH

Adolescent
Adult
Apolipoproteins
Biomarkers
Body Mass Index
Cross-Over Studies
Dietary Fats
Eating
Enzyme-Linked Immunosorbent Assay
Fasting
Fatty Acids, Monounsaturated
Fatty Acids, Unsaturated
Humans
Male
Middle Aged
Reference Values
Sensitivity and Specificity
Triglycerides

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10726104

Citation

Lovegrove, J A., et al. "Markers of Intestinally-derived Lipoproteins: Application to Studies of Altered Diet and Meal Fatty Acid Compositions." Nutrition, Metabolism, and Cardiovascular Diseases : NMCD, vol. 9, no. 1, 1999, pp. 9-18.
Lovegrove JA, Jackson KG, Murphy MC, et al. Markers of intestinally-derived lipoproteins: application to studies of altered diet and meal fatty acid compositions. Nutr Metab Cardiovasc Dis. 1999;9(1):9-18.
Lovegrove, J. A., Jackson, K. G., Murphy, M. C., Brooks, C. N., Zampelas, A., Knapper, J. M., ... Williams, C. M. (1999). Markers of intestinally-derived lipoproteins: application to studies of altered diet and meal fatty acid compositions. Nutrition, Metabolism, and Cardiovascular Diseases : NMCD, 9(1), pp. 9-18.
Lovegrove JA, et al. Markers of Intestinally-derived Lipoproteins: Application to Studies of Altered Diet and Meal Fatty Acid Compositions. Nutr Metab Cardiovasc Dis. 1999;9(1):9-18. PubMed PMID: 10726104.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Markers of intestinally-derived lipoproteins: application to studies of altered diet and meal fatty acid compositions. AU - Lovegrove,J A, AU - Jackson,K G, AU - Murphy,M C, AU - Brooks,C N, AU - Zampelas,A, AU - Knapper,J M, AU - Wright,J W, AU - Gould,B J, AU - Williams,C M, PY - 2000/3/22/pubmed PY - 2000/4/15/medline PY - 2000/3/22/entrez SP - 9 EP - 18 JF - Nutrition, metabolism, and cardiovascular diseases : NMCD JO - Nutr Metab Cardiovasc Dis VL - 9 IS - 1 N2 - BACKGROUND AND AIM: The atherogenic potential of dietary derived lipids, chylomicrons (CM) and their remnants (CMr) is now becoming more widely recognised. To investigate factors effecting levels of CM and CMr and their importance in coronary heart disease risk it is essential to use a specific method of quantification. Two studies were carried out to investigate: (i) effects of increased daily intake of long chain n-3 polyunsaturated fatty acid (LC n-3 PUFA), and (ii) effects of increasing meal monounsaturated fatty acid (MUFA) content on the postprandial response of intestinally-derived lipoproteins. The contribution of the intestinally-derived lipoproteins to total lipaemia was assessed by triacylglycerol-rich lipoprotein (TRL) apolipoprotein B-48 (apo B-48) and retinyl ester (RE) concentrations. METHODS AND RESULTS: In a randomised controlled crossover trial (placebo vs LC n-3 PUFA) a mean daily intake of 1.4 g/day of LC n-3 PUFA failed to reduce fasting and postprandial triacylglycerol (TAG) response in 9 healthy male volunteers. Although the pattern and nature of the apo B-48 response was consistent with the TAG response following the two diets, the postprandial RE response differed on the LC n-3 PUFA diet with a lower early RE response and a delayed and more marked increase in RE in the late postprandial period compared with the control diet, but the differences did not reach levels of statistical significance. In the meal study there was no effect of MUFA/SFA content on the total lipaemic response to the meals nor on the contribution of intestinally derived lipoproteins evaluated as TAG, apo B-48 and RE responses in the TRL fraction. In both studies, the RE and apo B-48 measurements provided broadly similar information with respect to lack of effects of dietary or meal fatty acid composition and the presence of single or multiple peak responses. However the apo B-48 and RE measurements differed with respect to the timing of their peak response times, with a delayed RE peak, relalive to apo B-48, of approximately 2-3 hours for the LC n-3 PUFA diet (p = 0.002) study and 1-1.5 hours for the meal MUFA/SFA study. CONCLUSIONS: It was concluded that there are limitations of using RE as a specific CM marker, apo B-48 quantitation was found to be a more appropriate method for CM and CMr quantitation. However it was still considered of value to measure RE as it provided additional information regarding the incorporation of other constituents into the CM particle. SN - 0939-4753 UR - https://www.unboundmedicine.com/medline/citation/10726104/Markers_of_intestinally_derived_lipoproteins:_application_to_studies_of_altered_diet_and_meal_fatty_acid_compositions_ L2 - https://medlineplus.gov/dietaryfats.html DB - PRIME DP - Unbound Medicine ER -