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Modifications of alpha-tocopherol and fatty acid concentrations in blood and adipose tissue of obese patients during a weight loss programme.
Nutr Metab Cardiovasc Dis. 1999 Feb; 9(1):25-32.NM

Abstract

BACKGROUND AND AIM

The aim of the study was to describe qualitative and/or quantitative modifications of lipoproteins, including their fatty acid composition, in obese patients during a hypocaloric diet, and determine whether the variations observed paralleled modifications of alpha-tocopherol concentration in adipose tissue and blood.

METHODS AND RESULTS

15 healthy, obese volunteers (5 males, 10 females; age: 32-69 yr; BMI: 28.4-60.5 kg/m2) were given a 3-week hypocaloric diet (3.9 MJ (941 kcal)). Adipose tissue and blood samples were taken at the beginning and at the end of this period. At baseline and after 3 weeks measurements were made for alpha-tocopherol and fatty acids in total serum, lipoproteins and adipose tissue; thiobarbituric acid-reactive substances (TBARS) in serum. A significant drop in cholesterol-rich particles (LDL and HDL) was observed, in parallel to a significant enrichment of n-6 polyunsaturated fatty acids (PUFA) at the expense of both saturated and monounsaturated fatty acids in serum. A drop in alpha-tocopherol concentration (expressed as mumol alpha-tocopherol/g lipid) in serum and lipoprotein fractions paralleled the decrease in cholesterol-rich lipoproteins.

CONCLUSIONS

Our results suggest that a hypocaloric diet not only decreases cholesterol-rich particle levels in serum, but also leads to a significant modification of fatty acid composition which may reflect improvement of insulin sensitivity. We did not observe any modification in adipose tissue after diet with regard to both alpha-tocopherol and fatty acid concentrations. Despite a drop in alpha-tocopherol concentration and an increase in n-6 PUFA content in serum, we did not find any enhancement of serum lipid peroxidation level evaluated by the thiobarbituric acid-reactive substance (TBARS) assay. If we assume that dietary intakes of alpha-tocopherol were not modified during this diet, it can be supposed that adipose tissue released alpha-tocopherol without any specific regulation, in parallel to the release of fatty acids.

Authors+Show Affiliations

Laboratoire de Biochimie, Hôpital de la Salpêtrière, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Controlled Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10726106

Citation

Bonnefont-Rousselot, D, et al. "Modifications of Alpha-tocopherol and Fatty Acid Concentrations in Blood and Adipose Tissue of Obese Patients During a Weight Loss Programme." Nutrition, Metabolism, and Cardiovascular Diseases : NMCD, vol. 9, no. 1, 1999, pp. 25-32.
Bonnefont-Rousselot D, Lepage S, Thérond P, et al. Modifications of alpha-tocopherol and fatty acid concentrations in blood and adipose tissue of obese patients during a weight loss programme. Nutr Metab Cardiovasc Dis. 1999;9(1):25-32.
Bonnefont-Rousselot, D., Lepage, S., Thérond, P., Bastard, J. P., Hainque, B., Turpin, G., Legrand, A., Delattre, J., & Bruckert, E. (1999). Modifications of alpha-tocopherol and fatty acid concentrations in blood and adipose tissue of obese patients during a weight loss programme. Nutrition, Metabolism, and Cardiovascular Diseases : NMCD, 9(1), 25-32.
Bonnefont-Rousselot D, et al. Modifications of Alpha-tocopherol and Fatty Acid Concentrations in Blood and Adipose Tissue of Obese Patients During a Weight Loss Programme. Nutr Metab Cardiovasc Dis. 1999;9(1):25-32. PubMed PMID: 10726106.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modifications of alpha-tocopherol and fatty acid concentrations in blood and adipose tissue of obese patients during a weight loss programme. AU - Bonnefont-Rousselot,D, AU - Lepage,S, AU - Thérond,P, AU - Bastard,J P, AU - Hainque,B, AU - Turpin,G, AU - Legrand,A, AU - Delattre,J, AU - Bruckert,E, PY - 2000/3/22/pubmed PY - 2000/4/15/medline PY - 2000/3/22/entrez SP - 25 EP - 32 JF - Nutrition, metabolism, and cardiovascular diseases : NMCD JO - Nutr Metab Cardiovasc Dis VL - 9 IS - 1 N2 - BACKGROUND AND AIM: The aim of the study was to describe qualitative and/or quantitative modifications of lipoproteins, including their fatty acid composition, in obese patients during a hypocaloric diet, and determine whether the variations observed paralleled modifications of alpha-tocopherol concentration in adipose tissue and blood. METHODS AND RESULTS: 15 healthy, obese volunteers (5 males, 10 females; age: 32-69 yr; BMI: 28.4-60.5 kg/m2) were given a 3-week hypocaloric diet (3.9 MJ (941 kcal)). Adipose tissue and blood samples were taken at the beginning and at the end of this period. At baseline and after 3 weeks measurements were made for alpha-tocopherol and fatty acids in total serum, lipoproteins and adipose tissue; thiobarbituric acid-reactive substances (TBARS) in serum. A significant drop in cholesterol-rich particles (LDL and HDL) was observed, in parallel to a significant enrichment of n-6 polyunsaturated fatty acids (PUFA) at the expense of both saturated and monounsaturated fatty acids in serum. A drop in alpha-tocopherol concentration (expressed as mumol alpha-tocopherol/g lipid) in serum and lipoprotein fractions paralleled the decrease in cholesterol-rich lipoproteins. CONCLUSIONS: Our results suggest that a hypocaloric diet not only decreases cholesterol-rich particle levels in serum, but also leads to a significant modification of fatty acid composition which may reflect improvement of insulin sensitivity. We did not observe any modification in adipose tissue after diet with regard to both alpha-tocopherol and fatty acid concentrations. Despite a drop in alpha-tocopherol concentration and an increase in n-6 PUFA content in serum, we did not find any enhancement of serum lipid peroxidation level evaluated by the thiobarbituric acid-reactive substance (TBARS) assay. If we assume that dietary intakes of alpha-tocopherol were not modified during this diet, it can be supposed that adipose tissue released alpha-tocopherol without any specific regulation, in parallel to the release of fatty acids. SN - 0939-4753 UR - https://www.unboundmedicine.com/medline/citation/10726106/Modifications_of_alpha_tocopherol_and_fatty_acid_concentrations_in_blood_and_adipose_tissue_of_obese_patients_during_a_weight_loss_programme_ L2 - https://medlineplus.gov/obesity.html DB - PRIME DP - Unbound Medicine ER -