Tags

Type your tag names separated by a space and hit enter

Regulation of the gene promoter for extracellular signal-regulated protein kinase 2 by transcription factors NF-Y and Sp3.
Biochem J. 2000 Apr 01; 347 Pt 1:155-61.BJ

Abstract

We have previously shown that the maximal promoter activity of the gene for extracellular signal-regulated protein kinase 2 (ERK2; also known as p42 mitogen-activated protein kinase) resides in the 371 bp 5'-flanking sequence. In the present study we defined roles for a CCAAT box and two adjacent GC boxes in the activity of this promoter. Deletion analysis and DNase I footprinting of this 371 bp region indicated that the CCAAT box at -64 and GC boxes at -86 and -39 are crucial for promoter activity. Electrophoretic mobility-shift assays showed that transcription factor NF-Y/CBF binds to the CCAAT box. Sp1 and Sp3, members of the Sp family of transcription factors, bind to the GC boxes of the ERK2 promoter. The binding of Sp3 was predominant over that of Sp1. Disruption by mutation of any of the CCAAT box and GC boxes similarly decreased promoter activity. These three cis elements exhibited a moderate synergy in promoter function. The transactivating role of NF-Y was corroborated by the finding that a dominant-negative form of NF-YA diminished the promoter activity. These results provide clues for refining our understanding of not only the regulation of expression of the gene for ERK2 but also mechanisms by which NF-Y and Sp1/Sp3 regulate transcription.

Authors+Show Affiliations

Department of Biochemistry I, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359-8513, Japan.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

10727414

Citation

Sugiura, N, and K Takishima. "Regulation of the Gene Promoter for Extracellular Signal-regulated Protein Kinase 2 By Transcription Factors NF-Y and Sp3." The Biochemical Journal, vol. 347 Pt 1, 2000, pp. 155-61.
Sugiura N, Takishima K. Regulation of the gene promoter for extracellular signal-regulated protein kinase 2 by transcription factors NF-Y and Sp3. Biochem J. 2000;347 Pt 1:155-61.
Sugiura, N., & Takishima, K. (2000). Regulation of the gene promoter for extracellular signal-regulated protein kinase 2 by transcription factors NF-Y and Sp3. The Biochemical Journal, 347 Pt 1, 155-61.
Sugiura N, Takishima K. Regulation of the Gene Promoter for Extracellular Signal-regulated Protein Kinase 2 By Transcription Factors NF-Y and Sp3. Biochem J. 2000 Apr 1;347 Pt 1:155-61. PubMed PMID: 10727414.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of the gene promoter for extracellular signal-regulated protein kinase 2 by transcription factors NF-Y and Sp3. AU - Sugiura,N, AU - Takishima,K, PY - 2000/3/23/pubmed PY - 2000/6/10/medline PY - 2000/3/23/entrez SP - 155 EP - 61 JF - The Biochemical journal JO - Biochem J VL - 347 Pt 1 N2 - We have previously shown that the maximal promoter activity of the gene for extracellular signal-regulated protein kinase 2 (ERK2; also known as p42 mitogen-activated protein kinase) resides in the 371 bp 5'-flanking sequence. In the present study we defined roles for a CCAAT box and two adjacent GC boxes in the activity of this promoter. Deletion analysis and DNase I footprinting of this 371 bp region indicated that the CCAAT box at -64 and GC boxes at -86 and -39 are crucial for promoter activity. Electrophoretic mobility-shift assays showed that transcription factor NF-Y/CBF binds to the CCAAT box. Sp1 and Sp3, members of the Sp family of transcription factors, bind to the GC boxes of the ERK2 promoter. The binding of Sp3 was predominant over that of Sp1. Disruption by mutation of any of the CCAAT box and GC boxes similarly decreased promoter activity. These three cis elements exhibited a moderate synergy in promoter function. The transactivating role of NF-Y was corroborated by the finding that a dominant-negative form of NF-YA diminished the promoter activity. These results provide clues for refining our understanding of not only the regulation of expression of the gene for ERK2 but also mechanisms by which NF-Y and Sp1/Sp3 regulate transcription. SN - 0264-6021 UR - https://www.unboundmedicine.com/medline/citation/10727414/Regulation_of_the_gene_promoter_for_extracellular_signal_regulated_protein_kinase_2_by_transcription_factors_NF_Y_and_Sp3_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/10727414/ DB - PRIME DP - Unbound Medicine ER -