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Fragmentation of human ceruloplasmin induced by hydrogen peroxide.
Biochimie. 2000 Feb; 82(2):175-80.B

Abstract

We investigated the fragmentation of human ceruloplasmin induced by H2O2 to study its oxidative damage. When ceruloplasmin was incubated with H2O2, the frequency of the protein fragmentation increased in a proportion to the concentration of H2O2. It also increased in a time-dependent manner and was accompanied by gradual loss of the oxidase activity. Hydroxyl radical scavengers such as azide and mannitol inhibited the fragmentation of ceruloplasmin. The deoxyribose assay showed that hydroxyl radicals were generated in the reaction of ceruloplasmin with H2O2. Incubation of ceruloplasmin with H2O2 resulted in a time-dependent release of copper ions. The released copper ion may participate in a Fenton-like reaction to produce hydroxyl radical, which enhanced the fragmentation. The protection of the fragmentation by copper chelators such as diethylenetriaminepentaacetic acid and bathocuproine indicates a role for copper ion in the reaction. These results suggest that the fragmentation of ceruloplasmin induced by H2O2 is due to hydroxyl radicals formed by a copper-dependent Fenton-like reaction.

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Authors+Show Affiliations

Choi SY
Department of Genetic Engineering, Division of Life Sciences, Hallym University, Chunchon, South Korea.
Kwon HY
No affiliation info available
Kwon OB
No affiliation info available
Eum WS
No affiliation info available
Kang JH
No affiliation info available

MeSH

CeruloplasminCopperCytochrome c GroupHumansHydrogen PeroxideHydroxyl RadicalOxidation-Reduction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

10727774

Citation

Choi, S Y., et al. "Fragmentation of Human Ceruloplasmin Induced By Hydrogen Peroxide." Biochimie, vol. 82, no. 2, 2000, pp. 175-80.
Choi SY, Kwon HY, Kwon OB, et al. Fragmentation of human ceruloplasmin induced by hydrogen peroxide. Biochimie. 2000;82(2):175-80.
Choi, S. Y., Kwon, H. Y., Kwon, O. B., Eum, W. S., & Kang, J. H. (2000). Fragmentation of human ceruloplasmin induced by hydrogen peroxide. Biochimie, 82(2), 175-80.
Choi SY, et al. Fragmentation of Human Ceruloplasmin Induced By Hydrogen Peroxide. Biochimie. 2000;82(2):175-80. PubMed PMID: 10727774.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fragmentation of human ceruloplasmin induced by hydrogen peroxide. AU - Choi,S Y, AU - Kwon,H Y, AU - Kwon,O B, AU - Eum,W S, AU - Kang,J H, PY - 2000/3/23/pubmed PY - 2000/5/20/medline PY - 2000/3/23/entrez SP - 175 EP - 80 JF - Biochimie JO - Biochimie VL - 82 IS - 2 N2 - We investigated the fragmentation of human ceruloplasmin induced by H2O2 to study its oxidative damage. When ceruloplasmin was incubated with H2O2, the frequency of the protein fragmentation increased in a proportion to the concentration of H2O2. It also increased in a time-dependent manner and was accompanied by gradual loss of the oxidase activity. Hydroxyl radical scavengers such as azide and mannitol inhibited the fragmentation of ceruloplasmin. The deoxyribose assay showed that hydroxyl radicals were generated in the reaction of ceruloplasmin with H2O2. Incubation of ceruloplasmin with H2O2 resulted in a time-dependent release of copper ions. The released copper ion may participate in a Fenton-like reaction to produce hydroxyl radical, which enhanced the fragmentation. The protection of the fragmentation by copper chelators such as diethylenetriaminepentaacetic acid and bathocuproine indicates a role for copper ion in the reaction. These results suggest that the fragmentation of ceruloplasmin induced by H2O2 is due to hydroxyl radicals formed by a copper-dependent Fenton-like reaction. SN - 0300-9084 UR - https://www.unboundmedicine.com/medline/citation/10727774/Fragmentation_of_human_ceruloplasmin_induced_by_hydrogen_peroxide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0300-9084(00)00380-1 DB - PRIME DP - Unbound Medicine ER -